CLC number:
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2020-03-01
Cited: 0
Clicked: 2018
Srikumar Krishnamoorthy, Zhengyi Zhang, Changxue Xu. Guided cell migration on a graded micropillar substrate[J]. Journal of Zhejiang University Science D, 2020, 3(1): 60-70.
@article{title="Guided cell migration on a graded micropillar substrate",
author="Srikumar Krishnamoorthy, Zhengyi Zhang, Changxue Xu",
journal="Journal of Zhejiang University Science D",
volume="3",
number="1",
pages="60-70",
year="2020",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-020-00059-7"
}
%0 Journal Article
%T Guided cell migration on a graded micropillar substrate
%A Srikumar Krishnamoorthy
%A Zhengyi Zhang
%A Changxue Xu
%J Journal of Zhejiang University SCIENCE D
%V 3
%N 1
%P 60-70
%@ 1869-1951
%D 2020
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-020-00059-7
TY - JOUR
T1 - Guided cell migration on a graded micropillar substrate
A1 - Srikumar Krishnamoorthy
A1 - Zhengyi Zhang
A1 - Changxue Xu
J0 - Journal of Zhejiang University Science D
VL - 3
IS - 1
SP - 60
EP - 70
%@ 1869-1951
Y1 - 2020
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-020-00059-7
Abstract: Cell migration is facilitated by the interaction of living cells and their local microenvironment. The local topography is
one of the key factors regulating cell migration. Interaction between the surface topography and the cell behaviors is critical to understanding tissue development and regeneration. In this study, a dynamic mask photolithography technique has
been utilized to fabricate a surface with graded micropillars. It has been demonstrated that the cells have been successfully
guided to migrate from the sparse zone to the dense zone. The cell polarization angle has been characterized in both sparse
zone and the dense zone. Compared to the dense zone, the cells in the sparse zone are more aligned along the direction of
the micropillar spacing gradient, which enables the guided cell migration. Moreover, the efects of the micropillar spacing
gradient, micropillar diameter, and micropillar height have been investigated in terms of the cell migration speed and cell
spreading area. Finally, two issues signifcantly afecting the cell migration have been discussed: trapped cells between the
micropillars and cell clusters.
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