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On-line Access: 2021-08-28
Received: 2021-04-21
Revision Accepted: 2021-08-10
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Utility of TPP‑manufactured biophysical restrictions to probe multiscale cellular dynamics
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Panyu Fei, Haibo Ding, Yu Duan, Xinyi Wang, Wei Hu, Peng Wu, Mengxiao Wei, Zhengchun Peng. Utility of TPP‑manufactured biophysical restrictions to probe multiscale cellular dynamics[J]. Journal of Zhejiang University Science D, 2021, 4(4): 776-789.
@article{title="Utility of TPP‑manufactured biophysical restrictions to probe multiscale cellular dynamics",
author="Panyu Fei, Haibo Ding, Yu Duan, Xinyi Wang, Wei Hu, Peng Wu, Mengxiao Wei, Zhengchun Peng",
journal="Journal of Zhejiang University Science D",
volume="4",
number="4",
pages="776-789",
year="2021",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-021-00163-2"
}
%0 Journal Article
%T Utility of TPP‑manufactured biophysical restrictions to probe multiscale cellular dynamics
%A Panyu Fei
%A Haibo Ding
%A Yu Duan
%A Xinyi Wang
%A Wei Hu
%A Peng Wu
%A Mengxiao Wei
%A Zhengchun Peng
%J Journal of Zhejiang University SCIENCE D
%V 4
%N 4
%P 776-789
%@ 1869-1951
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-021-00163-2
TY - JOUR
T1 - Utility of TPP‑manufactured biophysical restrictions to probe multiscale cellular dynamics
A1 - Panyu Fei
A1 - Haibo Ding
A1 - Yu Duan
A1 - Xinyi Wang
A1 - Wei Hu
A1 - Peng Wu
A1 - Mengxiao Wei
A1 - Zhengchun Peng
J0 - Journal of Zhejiang University Science D
VL - 4
IS - 4
SP - 776
EP - 789
%@ 1869-1951
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-021-00163-2
Abstract: Biophysical restrictions regulate protein difusion, nucleus deformation, and cell migration, which are all universal and
important processes for cells to perform their biological functions. However, current technologies addressing these multiscale questions are extremely limited. Herein, through two-photon polymerization (TPP), we present the precise, low-cost,
and multiscale microstructures (micro-fences) as a versatile investigating platform. With nanometer-scale printing resolution and multiscale scanning capacity, TPP is capable of generating micro-fences with sizes of 0.5–1000 μm. These microfences are utilized as biophysical restrictions to determine the fuidity of supported lipid bilayers (SLB), to investigate the
restricted difusion of Src family kinase protein Lck on SLB, and also to reveal the mechanical bending of cell nucleus and
T cell climbing ability. Taken together, the proposed versatile and low-cost micro-fences have great potential in probing the
restricted dynamics of molecules, organelles, and cells to understand the basics of physical biology.
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