CLC number:
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
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Farnaz Rahimi, Nima Ahmadkhani, Aida Goodarzi, Fariba Noori, Sajad Hassanzadeh, Sepideh Saghati, Mehdi Khanmohammadi & Arash Goodarzi. Gelatin-based hydrogel functionalized with taurine moieties for in vivo skin tissue regeneration[J]. Journal of Zhejiang University Science D, 2023, 6(3): 284-297.
@article{title="Gelatin-based hydrogel functionalized with taurine moieties for in vivo skin tissue regeneration",
author="Farnaz Rahimi, Nima Ahmadkhani, Aida Goodarzi, Fariba Noori, Sajad Hassanzadeh, Sepideh Saghati, Mehdi Khanmohammadi & Arash Goodarzi",
journal="Journal of Zhejiang University Science D",
volume="6",
number="3",
pages="284-297",
year="2023",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-022-00227-x"
}
%0 Journal Article
%T Gelatin-based hydrogel functionalized with taurine moieties for in vivo skin tissue regeneration
%A Farnaz Rahimi
%A Nima Ahmadkhani
%A Aida Goodarzi
%A Fariba Noori
%A Sajad Hassanzadeh
%A Sepideh Saghati
%A Mehdi Khanmohammadi & Arash Goodarzi
%J Journal of Zhejiang University SCIENCE D
%V 6
%N 3
%P 284-297
%@ 1869-1951
%D 2023
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-022-00227-x
TY - JOUR
T1 - Gelatin-based hydrogel functionalized with taurine moieties for in vivo skin tissue regeneration
A1 - Farnaz Rahimi
A1 - Nima Ahmadkhani
A1 - Aida Goodarzi
A1 - Fariba Noori
A1 - Sajad Hassanzadeh
A1 - Sepideh Saghati
A1 - Mehdi Khanmohammadi & Arash Goodarzi
J0 - Journal of Zhejiang University Science D
VL - 6
IS - 3
SP - 284
EP - 297
%@ 1869-1951
Y1 - 2023
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-022-00227-x
Abstract: Functionalized hydrogels stimulate the migration and morphogenesis of endothelial cells (ECs) and are useful substrates for wound healing. The present study investigates the feasibility of covalent conjugation of taurine (Tau) on a gelatin-based hydrogel. This hydrogel is expected to maintain positive charged growth factors such as basic fibroblast growth factor (bFGF) and vascular endothelial growth factors (VEGFs) near ECs within the hydrogel microenvironment. The gelatin was conjugated with hydroxyl phenol (Ph) and Tau moieties, and in following that Ph residues were crosslinked through a horseradish peroxidase-catalyzed reaction. The migration characteristics of ECs were analyzed by scratch migration assay and microparticle-based cell migration assay. Cellular morphology and amounts of angiopoietin 1 (Ang 1), bFGF, and VEGF proteins were evaluated for encapsulated cells. The potential of synthesized hydrogels in wound healing was assessed by the percentage of reduction from the original wound size and histopathological analyses of rat skin. The incorporated Tau molecules within the hydrogel remained stable through covalent bonds during incubation. During extended incubation, the gelatin-based hydrogel conjugated with Tau improved the migration distance and number of existing migrated ECs. Immobilized Tau within the gelatin-based hydrogel induced high motility of ECs, accompanied by robust cytoskeleton extension and a cell subpopulation that expressed CD44 and CD31 receptors as well as enhancement of Ang 1, bFGF, and VEGF. We found that injectable Gel-Ph-Tau effectively improves wound-healing parameters.
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