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Journal of Zhejiang University SCIENCE  Vol. No. P.

http://doi.org/10.1007/s42242-024-00287-1


Ca(V)3.3-Mediated Endochondral Ossification in a Threedimensional Bioprinted GelMA hydrogel


Author(s):  Zhi Wang, Xin Wang, Yang Huang, Junjun Yang, Zu Wan, Zhenlan Fu, Xiaoyuan Gong, Guangxing Chen, Liu Yang

Affiliation(s):  Chongqing Key Laboratory of Precision Medicine in Joint Surgery, Center for Joint Surgery, Southwest Hospital, Army Medical University (Third Military Medical University), Chongqing, 400038, China; more

Corresponding email(s):   sliegxy@foxmail.com (Xiaoyuan Gong* ), cgx7676@hotmail.com (Guangxing Chen**), and jointsurgery@163.com (Liu Yang***)

Key Words:  bone organoid, endochondral ossification, T-VDCC, Ca(V)3.3, 3D bioprinting


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Zhi Wang, Xin Wang, Yang Huang, Junjun Yang, Zu Wan, Zhenlan Fu, Xiaoyuan Gong, Guangxing Chen, Liu Yang. Ca(V)3.3-Mediated Endochondral Ossification in a Threedimensional Bioprinted GelMA hydrogel[J]. Journal of Zhejiang University Science , , (): .

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Abstract: 
The growth plate (GP) is a crucial tissue involved in skeleton development via endochondral ossification (EO). The bone organoid is a potential research model capable of simulating the physiological function, spatial structure, and intercellular communication of native GPs. However, mimicking the EO process remains a key challenge for bone organoid research. To simulate this orderly mineralization process, we designed an in vitro shca(V)3.3 ATDC5-loaded gelatin methacryloyl (GelMA) hydrogel model and evaluated its bioprintability for future organoid construction. In this paper, we report the first demonstration that the T-type voltage-dependent calcium channel (t-VDCC) subtype ca(V)3.3 is dominantly expressed in chondrocytes and is negatively correlated with the hypertrophic differentiation of chondrocytes during the EO process. Furthermore, ca(V)3.3 knockdown chondrocytes loaded with the GelMA hydrogel successfully captured the EO process and provide a bioink capable of constructing layered and orderly mineralized GP organoids in the future. The results of this study could therefore provide a potential target for regulating the EO process and a novel strategy for simulating it in bone organoids.

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