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Banafsheh Dolatyar, Bahman Zeynali, Iman Shabani, Azita Parvaneh Tafreshi, Reza Karimi-Soflou. Enhanced axonal regeneration and functional recovery of the injured sciatic nerve in a rat model by lithium-loaded electrospun nanofibrous scaffolds[J]. Journal of Zhejiang University Science D, 2016, -1(-1): .
@article{title="Enhanced axonal regeneration and functional recovery of the injured
sciatic nerve in a rat model by lithium-loaded electrospun nanofibrous
scaffolds",
author="Banafsheh Dolatyar, Bahman Zeynali, Iman Shabani, Azita Parvaneh Tafreshi, Reza Karimi-Soflou",
journal="Journal of Zhejiang University Science D",
volume="-1",
number="-1",
pages="",
year="2016",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-024-00304-3"
}
%0 Journal Article
%T Enhanced axonal regeneration and functional recovery of the injured
sciatic nerve in a rat model by lithium-loaded electrospun nanofibrous
scaffolds
%A Banafsheh Dolatyar
%A Bahman Zeynali
%A Iman Shabani
%A Azita Parvaneh Tafreshi
%A Reza Karimi-Soflou
%J Journal of Zhejiang University SCIENCE D
%V -1
%N -1
%P
%@ 1869-1951
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-024-00304-3
TY - JOUR
T1 - Enhanced axonal regeneration and functional recovery of the injured
sciatic nerve in a rat model by lithium-loaded electrospun nanofibrous
scaffolds
A1 - Banafsheh Dolatyar
A1 - Bahman Zeynali
A1 - Iman Shabani
A1 - Azita Parvaneh Tafreshi
A1 - Reza Karimi-Soflou
J0 - Journal of Zhejiang University Science D
VL - -1
IS - -1
SP -
EP -
%@ 1869-1951
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-024-00304-3
Abstract: Increasing evidence indicates that engineered nerve grafts have great potential for the
regeneration of peripheral nerve injuries (PNIs). While most studies have focused only on the
topographical features of the grafts, we have considered both the biophysical and biochemical
manipulations in our applied nanoscaffold. To achieve this, we fabricated an electrospun
nanofibrous scaffold (ENS) containing polylactide nanofibers loaded with lithium (Li) ions, a
Wnt/??catenin signaling activator. In addition, we seeded human adipose-derived
mesenchymal stem cells (hADMSCs) onto this engineered scaffold to examine if their
differentiation toward Schwann-like cells was induced. We further examined the efficacy of
the scaffolds for nerve regeneration in vivo via grafting in a PNI rat model. Our results
showed that Li-loaded ENSs gradually released Li within 11 d, at concentrations ranging
from 0.02 to (3.640.10) mmol/L, and upregulated the expression of Wnt/?-catenin target
genes (cyclinD1 and c-Myc) as well as those of Schwann cell markers (growth associated
protein 43 (GAP43), S100 calcium binding protein B (S100B), glial fibrillary acidic protein
(GFAP), and SRY-box transcription factor 10 (SOX10)) in differentiated hADMSCs. In the
PNI rat model, implantation of Li-loaded ENSs with/without cells improved behavioral
features such as sensory and motor functions as well as the electrophysiological
characteristics of the injured nerve. This improved function was further validated by
histological analysis of sciatic nerves grafted with Li-loaded ENSs, which showed no fibrous
connective tissue but enhanced organized myelinated axons. The potential of Li-loaded ENSs
in promoting schwann cell differentiation of hADMSCs and axonal regeneration of injured
sciatic nerves suggests their potential for application in peripheral nerve tissue engineering.
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