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On-line Access: 2024-07-20
Received: 2024-01-02
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Microfluidic thermotaxic selection of highly motile sperm and in vitro fertilization
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Sihan Chen, Jiemin Chen, Zihan Qin, Jibo Wang, Yuwen Wang, Rong Liu, Wen Zhao, Ming Zhang, Yuanzhen Zhang, Mengcheng Luo, Pu Chen. Microfluidic thermotaxic selection of highly motile sperm and in vitro fertilization[J]. Journal of Zhejiang University Science D, 2024, 7(5): 687-700.
@article{title="Microfluidic thermotaxic selection of highly motile sperm and in vitro
fertilization",
author="Sihan Chen, Jiemin Chen, Zihan Qin, Jibo Wang, Yuwen Wang, Rong Liu, Wen Zhao, Ming Zhang,
Yuanzhen Zhang, Mengcheng Luo, Pu Chen",
journal="Journal of Zhejiang University Science D",
volume="7",
number="5",
pages="687-700",
year="2024",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-024-00306-1"
}
%0 Journal Article
%T Microfluidic thermotaxic selection of highly motile sperm and in vitro
fertilization
%A Sihan Chen
%A Jiemin Chen
%A Zihan Qin
%A Jibo Wang
%A Yuwen Wang
%A Rong Liu
%A Wen Zhao
%A Ming Zhang
%A
Yuanzhen Zhang
%A Mengcheng Luo
%A Pu Chen
%J Journal of Zhejiang University SCIENCE D
%V 7
%N 5
%P 687-700
%@ 1869-1951
%D 2024
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-024-00306-1
TY - JOUR
T1 - Microfluidic thermotaxic selection of highly motile sperm and in vitro
fertilization
A1 - Sihan Chen
A1 - Jiemin Chen
A1 - Zihan Qin
A1 - Jibo Wang
A1 - Yuwen Wang
A1 - Rong Liu
A1 - Wen Zhao
A1 - Ming Zhang
A1 -
Yuanzhen Zhang
A1 - Mengcheng Luo
A1 - Pu Chen
J0 - Journal of Zhejiang University Science D
VL - 7
IS - 5
SP - 687
EP - 700
%@ 1869-1951
Y1 - 2024
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-024-00306-1
Abstract: The selection of the most motile and functionally competent sperm is an essential basis for in vitro fertilization (IVF) and
normal embryonic development. Widely adopted clinical approaches for sperm sample processing intensely rely on centrifu gation and wash steps that may induce mechanical damage and oxidative stress to sperm. Although a few microfluidic sperm
sorting devices may avoid these adverse effects by exploiting intrinsic guidance mechanisms of sperm swimming, none of
these approaches have been fully validated by clinical-grade assessment criteria. In this study, a microfluidic sperm sorting
device that enables the selection of highly motile and functional sperm via their intrinsic thermotaxis is presented. Bioin spired by the temperature microenvironment in the fallopian tube during natural sperm selection, a microfluidic device with
controllable temperature gradients along the sperm separation channel was designed and fabricated. This study investigated
the optimal temperature conditions for human sperm selection and fully characterized thermotaxis-selected sperm with 45
human sperm samples. Results indicated that a temperature range of 35–36.5 °C along the separation channel significantly
improves human sperm motility rate ((85.25±6.28)% vs. (60.72±1.37)%; P=0.0484), increases normal sperm morphology
rate ((16.42±1.43)% vs. (12.55±0.88)%; P<0.0001), and reduces DNA fragmentation ((7.44±0.79)% vs. (10.36±0.72)%;
P=0.0485) compared to the nonthermotaxis group. Sperm thermotaxis is species-specific, and selected mouse sperm dis played the highest motility in response to a temperature range of 36–37.5 °C along the separation channel. Furthermore, IVF
experiments indicated that the selected sperm permitted an increased fertilization rate and improved embryonic development
from zygote to blastocyst. This microfluidic thermotaxic selection approach will be translated into clinical practice to improve
the IVF success rate for patients with oligozoospermia and asthenozoospermia.
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