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Bio-Design and Manufacturing  2016 Vol.-1 No.-1 P.

http://doi.org/10.1007/s42242-024-00312-3


Electrospun Polyvinyl Alcohol Fibres Incorporating an Antimicrobial Gel for Enzymatically Controlled Reactive Oxygen Species Release


Author(s):  Joel Yupanqui Mieles, Cian Vyas, Evangelos Daskalakis, Mohamed Hassan, James Birkett, Abdalla M. Omar, Gavin Humphreys, Carl Diver, Paulo Bartolo

Affiliation(s):  Department of Mechanical, Aerospace and Civil Engineering, University of Manchester, Manchester M13 9PL, UK; more

Corresponding email(s):   joel.yupanquimieles@manchester.ac.uk (J.Y.M.), cian.vyas@ntu.edu.sg (C.V), pbartolo@ntu.edu.sg (P.B.)

Key Words:  antimicrobial, electrospinning, hydrogen peroxide, polyvinyl alcohol, reactive oxygen species, tissue engineering, wound dressing


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Joel Yupanqui Mieles , Cian Vyas, Evangelos Daskalakis , Mohamed Hassan , James Birkett , Abdalla M. Omar , Gavin Humphreys , Carl Diver , Paulo Bartolo. Electrospun Polyvinyl Alcohol Fibres Incorporating an Antimicrobial Gel for Enzymatically Controlled Reactive Oxygen Species Release[J]. Journal of Zhejiang University Science D, 2016, -1(-1): .

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Abstract: 
Wounds pose a risk to the skin, our body’s primary defence against infections. The rise of antibiotic resistance has prompted the development of novel therapies. RO-101® is an antimicrobial gel that delivers therapeutic levels of hydrogen peroxide (H2O2), a reactive oxygen species, directly to the wound bed. In this study, electrospinning was used to incorporate RO-101® into a polyvinyl alcohol (PVA) submicron fibrous mesh that can act as a delivery agent, achieve a sustained release profile, and provide a barrier against infection. Adequate incorporation of this gel into submicron fibres was confirmed via nuclear magnetic resonance spectroscopy. Furthermore, scanning electron microscopy exhibited smooth and uniform meshes with diameters in the 200–500 nm range. PVA/RO-101 electrospun meshes generated H2O2 in concentrations exceeding 1 mM/(mL∙g) (1 mM=1 mmol/L) after 24 h, and the role of sterilisation on H2O2 release was evaluated. PVA/RO-101 meshes exhibited Preprint of Bio-Design and Manufacturing (unedited) antimicrobial activity against both Gram-positive Staphylococcus aureus (S. aureus) and Gramnegative Pseudomonas aeruginosa (P. aeruginosa) bacteria, achieving viable count reductions of up to 1 log unit CFU/mm2 (CFU: colony-forming units). Moreover, these meshes were capable of disrupting biofilm formation, even against multidrug-resistant organisms such as methicillin-resistant S. aureus (MRSA). Furthermore, increasing the RO-101® concentration resulted in higher H2O2 production and an enhanced antimicrobial effect, while fibroblast cell viability and proliferation tests showed a concentration-dependent response with high cytocompatibility at low RO-101® concentrations. This study therefore demonstrates the potential of highly absorbent PVA/RO-101 meshes as potential antimicrobial wound dressings.

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