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Bio-Design and Manufacturing  2016 Vol.-1 No.-1 P.

http://doi.org/10.1007/s42242-BDMJ-D-24-00011


Concurrent bioprinted scaffold with autologous bone and allogeneic BMSCs promote bone regeneration via recuiting native BMSCs


Author(s):  Yu Huan, Hongqing Chen, Dezhi Zhou, Xin He, Sanzhong Li, Xiuquan Wu, Bo Jia, Yanan Dou, Xiaowei Fei, Shuang Wu, Zhou Fei, Tao Xu, Fei Fei

Affiliation(s):  Department of Neurosurgery, Xijing Hospital, Air Force Medical University, Xi'an, 710032, China; more

Corresponding email(s):   feeplus@163.com, xut@tsinghua-sz.org, feizhou@fmmu.edu.cn

Key Words: 


Yu Huan, Hongqing Chen, Dezhi Zhou, Xin He, Sanzhong Li, Xiuquan Wu, Bo Jia,Yanan Dou, Xiaowei Fei, Shuang Wu, Zhou Fei, Tao Xu,Fei Fei. Concurrent bioprinted scaffold with autologous bone and allogeneic BMSCs promote bone regeneration via recuiting native BMSCs[J]. Journal of Zhejiang University Science D, 2016, -1(-1): .

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author="Yu Huan, Hongqing Chen, Dezhi Zhou, Xin He, Sanzhong Li, Xiuquan Wu, Bo Jia,Yanan Dou, Xiaowei Fei, Shuang Wu, Zhou Fei, Tao Xu,Fei Fei",
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publisher="Zhejiang University Press & Springer",
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%T Concurrent bioprinted scaffold with autologous bone and allogeneic BMSCs promote bone regeneration via recuiting native BMSCs
%A Yu Huan
%A Hongqing Chen
%A Dezhi Zhou
%A Xin He
%A Sanzhong Li
%A Xiuquan Wu
%A Bo Jia
%A Yanan Dou
%A Xiaowei Fei
%A Shuang Wu
%A Zhou Fei
%A Tao Xu
%A Fei Fei
%J Journal of Zhejiang University SCIENCE D
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%I Zhejiang University Press & Springer
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T1 - Concurrent bioprinted scaffold with autologous bone and allogeneic BMSCs promote bone regeneration via recuiting native BMSCs
A1 - Yu Huan
A1 - Hongqing Chen
A1 - Dezhi Zhou
A1 - Xin He
A1 - Sanzhong Li
A1 - Xiuquan Wu
A1 - Bo Jia
A1 - Yanan Dou
A1 - Xiaowei Fei
A1 - Shuang Wu
A1 - Zhou Fei
A1 - Tao Xu
A1 - Fei Fei
J0 - Journal of Zhejiang University Science D
VL - -1
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EP -
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PB - Zhejiang University Press & Springer
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DOI - 10.1007/s42242-BDMJ-D-24-00011


Abstract: 
Autologous bone marrow-derived mesenchymal stem cells (BMSCs) have been shown to promote osteogenesis; however, whether allogeneic BMSCs (Allo-BMSCs) have effects on bone regeneration is not clear. Therefore, we explored the effect of Allo-BMSCs on promoting bone regeneration in 3D-printed autologous bone (AB) scaffolds. Firstly, we concurrently printed scaffolds with polycaprolactone, AB particles (ABP) and Allo-BMSCs for appropriate support, providing bioactive factors and seed cells to promote osteogenesis. In vitro studies showed that AB scaffolds promoted the osteogenic differentiation of Allo-BMSCs. In vivo studies revealed that implantation of scaffolds loaded with ABP and Allo-BMSCs into canine skull defects for 9 months promoted osteogenesis. Further studies suggested that only a small portion of implanted Allo-BMSCs survived and became the vascular endothelial cell, chondrocyte, and osteocyte, and the implanted Allo-BMSCs released stromal cell-derived factor 1 through paracrine signaling to recruit native BMSCs into the defect, promoting bone regeneration. This study provides additional opportunities for the future uses of Allo-BMSCs.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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