Full Text:   <2542>

CLC number: Q814.3

On-line Access: 

Received: 2002-03-06

Revision Accepted: 2002-06-30

Crosschecked: 0000-00-00

Cited: 17

Clicked: 4589

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE A 2002 Vol.3 No.5 P.563-566


Fiber optic biosensor of immobilized firefly luciferase

Author(s):  CAI Jin, MENG Wen-fang, JI Xin-song

Affiliation(s):  College of Material & Chemical Engineering, Zhejiang University, Hangzhou 310027, China; more

Corresponding email(s):   caij@che.zju.edu.cn

Key Words:  Firefly luciferase, ATP, Immobilized enzyme, Fiber optic biosensor

Share this article to: More

CAI Jin, MENG Wen-fang, JI Xin-song. Fiber optic biosensor of immobilized firefly luciferase[J]. Journal of Zhejiang University Science A, 2002, 3(5): 563-566.

@article{title="Fiber optic biosensor of immobilized firefly luciferase",
author="CAI Jin, MENG Wen-fang, JI Xin-song",
journal="Journal of Zhejiang University Science A",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Fiber optic biosensor of immobilized firefly luciferase
%A CAI Jin
%A MENG Wen-fang
%A JI Xin-song
%J Journal of Zhejiang University SCIENCE A
%V 3
%N 5
%P 563-566
%@ 1869-1951
%D 2002
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2002.0563

T1 - Fiber optic biosensor of immobilized firefly luciferase
A1 - CAI Jin
A1 - MENG Wen-fang
A1 - JI Xin-song
J0 - Journal of Zhejiang University Science A
VL - 3
IS - 5
SP - 563
EP - 566
%@ 1869-1951
Y1 - 2002
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2002.0563

Luciferase from firefly lantern extract was immobilized on CNBr-activated Sepharose 4B. The kinetic properties of immobilized luciferase were extensively studied. The Km′ for D-luciferin is 11.9 μmol/L, the optimum pH and temperature for Sepharose-bound enzyme were 7.8 and 25°C respectively. A luminescence fiber optic biosensor, making use of immobilized crude luciferase, was developed for assay of ATP. The peak light intensity was linear with respect to ATP concentration in range of 10-9–10-5 mol/L. A biological application was also demonstrated with the determination of serum ATP from rats bred in low versus normal oxygen environments.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Blum, L.J., Gautier, S.M., Coulet, P.R., 1988. Luminescence fiber optic biosensor. Anal. Lett., 21: 717-726.

[2] Lagido, C., Pettitt, J., Porter, A.J.R., Paton, G.I., Glover, L.A., 2001. Development and application of bioluminescent Caenorhabditis elegans as multicellular eukaryotic biosensors. FEBS Letters, 493:36-39.

[3] Leach, F.R., 1981. ATP determination with firefly luciferase. J. Appl. Biochem., 3: 473-478.

[4] Lee,Y., Jablonski, I., Deluca, M., 1981. Immobilization of firefly luciferase on glass rods: Properties of the immobilized enzyme. Anal. Biochem., 80:496-500.

[5] Ji, X.S., Li, H.X., Yuan, Z.Y., Liu S.H., 1984. Immobilization of NAD kinase. Enzyme Engineering 7, Anal. N. Y. Acad. Sci., 434:264-266.

[6] Maria, P. X., Begona, V., Maria, D. M., 2000. Fiber optic monitoring of carbamate pesticides using porous glass with covalently bound chlorophenol red. Biosensors & Bioelectronics, 14:895-905.

[7] Michel, P.E., Gautier-Sauvigne, S.M., Blum, L.J., 1998. A transient enzymatic inhibition as an efficient tool for the discriminating bioluminescent analysis of three adenylic nucleotides with a fiberoptic sensor based on a compartmentalized tri-enzymatic sensing layer. Analytica Chimica Acta, 360: 89-99.

[8] Neuberg, C., 1944. Convenient method for deproteinization. Arch. Biochem. 4:101-105.

[9] Tservistas, M., Koneke, R., Comte, A. Scheper T., 2001. Oxygen monitoring in supercritical carbon dioxide using a fibre optic sensor. Enzyme and Microbial Technology, 28:637-634.

[10] Vangelis, G. A., Yannis, D. C., 2002. A portable fiber-optic pesticide biosensor based on immobilized cholinesterase and sol-gel entrapped bromcresol purple for in-field use. Biosensor & Bioelectronics, 17:61-69.

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE