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Bio-Design and Manufacturing  2021 Vol.4 No.1 P.114~120

10.1631/jzus.2003.0114


Study on the effect of doxorubicin on expressions of genes encoding myocardial sarcoplasmic reticulum Ca2+ transport proteins and the effect of taurine on myocardial protection in rabbits


Author(s):  HUANG Xian-mei, ZHU Wei-hua, KANG Man-li

Affiliation(s):  Children's Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China

Corresponding email(s):   hxianmei@hotmail.com

Key Words:  Doxorubicin, Ca2+-ATPase, Ryanodine receptor, Taurine, Gene expression


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HUANG Xian-mei, ZHU Wei-hua, KANG Man-li. Study on the effect of doxorubicin on expressions of genes encoding myocardial sarcoplasmic reticulum Ca2+ transport proteins and the effect of taurine on myocardial protection in rabbits[J]. Journal of Zhejiang University Science D, 2021, 4(1): 114~120.

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author="HUANG Xian-mei, ZHU Wei-hua, KANG Man-li",
journal="Journal of Zhejiang University Science D",
volume="4",
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pages="114~120",
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publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2003.0114"
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%T Study on the effect of doxorubicin on expressions of genes encoding myocardial sarcoplasmic reticulum Ca2+ transport proteins and the effect of taurine on myocardial protection in rabbits
%A HUANG Xian-mei
%A ZHU Wei-hua
%A KANG Man-li
%J Journal of Zhejiang University SCIENCE D
%V 4
%N 1
%P 114~120
%@ 1869-1951
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2003.0114

TY - JOUR
T1 - Study on the effect of doxorubicin on expressions of genes encoding myocardial sarcoplasmic reticulum Ca2+ transport proteins and the effect of taurine on myocardial protection in rabbits
A1 - HUANG Xian-mei
A1 - ZHU Wei-hua
A1 - KANG Man-li
J0 - Journal of Zhejiang University Science D
VL - 4
IS - 1
SP - 114
EP - 120
%@ 1869-1951
Y1 - 2021
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2003.0114


Abstract: 
To investigate the effect of doxorubicin (DOX) on gene expression of the myocardial sarcoplasmic reticulum (SR) Ca2+ transport proteins and the mechanism of taurine (Tau) protecting cardiac muscle cells, 9 rabbits were injected with DOX, 8 rabbits with DOX and Tau, and 9 rabbits with normal saline. Cardiac function, concentration of calcium in cardiomyocytes (Myo[Ca2+]i), activity of SR ca2+-ATPase(SERCA2a), level of SERCA2a mRNA and Ca2+ released channels(RYR2)mRNA were detected. The left ventricle tissues were observed by electron microscopy. The results showed that cardiac index, left ventricular systolic pressure, activity of SR ca2+-ATPase and level of SERCA2a mRNA decreased, while Myo[Ca2+]i increased in DOX-treated rabbits. DOX could not affect the level of RYR2 mRNA. Tau intervention could alleviate the increase of left ventricular diastolic pressure, Myo[Ca2+]i and the decrease of SERCA2a mRNA induced by doxorubicin. The results suggested that downregulation of SERCA2a gene expression was an important mechanism of DOX-induced cardiomyopathy and that Tau could partially improve the heart function by reducing calcium overload and alleviating downregulation of SERCA2a mRNA.

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