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Journal of Zhejiang University SCIENCE A 2003 Vol.4 No.2 P.136~141

http://doi.org/10.1631/jzus.2003.0136


Lysozyme refolding at high concentration by dilution and size-exclusion chromatography


Author(s):  GAO Yong-gui, GUAN Yi-xin, YAO Shan-jing, CHO Man-gi

Affiliation(s):  Department of Chemical and Biochemical Engineering; more

Corresponding email(s):   yaosj@che.zju.edu.cn

Key Words:  Lysozyme refolding, Downstream process, Dilution, Size exclusion chromatography, Urea


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GAO Yong-gui, GUAN Yi-xin, YAO Shan-jing, CHO Man-gi. Lysozyme refolding at high concentration by dilution and size-exclusion chromatography[J]. Journal of Zhejiang University Science A, 2003, 4(2): 136~141.

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author="GAO Yong-gui, GUAN Yi-xin, YAO Shan-jing, CHO Man-gi",
journal="Journal of Zhejiang University Science A",
volume="4",
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pages="136~141",
year="2003",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2003.0136"
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%A GAO Yong-gui
%A GUAN Yi-xin
%A YAO Shan-jing
%A CHO Man-gi
%J Journal of Zhejiang University SCIENCE A
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%P 136~141
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%D 2003
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2003.0136

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T1 - Lysozyme refolding at high concentration by dilution and size-exclusion chromatography
A1 - GAO Yong-gui
A1 - GUAN Yi-xin
A1 - YAO Shan-jing
A1 - CHO Man-gi
J0 - Journal of Zhejiang University Science A
VL - 4
IS - 2
SP - 136
EP - 141
%@ 1869-1951
Y1 - 2003
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2003.0136


Abstract: 
This study of renaturation by dilution and size exclusion chromatography (SEC) addition of urea to improve yield as well as the initial and final protein concentrations showed that although urea decreased the rate of lysozyme refolding, it could suppress protein aggregation to sustain the pathway of correct refolding at high protein concentration; and that there existed an optimum urea concentration in renaturation buffer. Under the above conditions, lysozyme was successfully refolded from initial concentration of up to 40 mg/mL by dilution and 100 mg/mL by SEC, with the yield of the former being more than 40% and that of the latter being 34.8%. Especially, under the condition of 30 min interval time, i.e. τ>2(tR2-tR1), the efficiency was increased by 25% and the renaturation buffer could be recycled for SEC refolding in continuous operation of downstream process.

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Reference

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houbin wang@peking university<zhejdx1@163.com>

2011-07-07 15:39:33

Very good the research is interesting

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