Full Text:   <1257>

CLC number: R377

On-line Access: 

Received: 2001-12-28

Revision Accepted: 2002-03-05

Crosschecked: 0000-00-00

Cited: 0

Clicked: 3727

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE A 2003 Vol.4 No.2 P.232~235

http://doi.org/10.1631/jzus.2003.0232


Purification and relationship with gastric disease of a 130 kDa (CagA) protein of Helicobacter pylori


Author(s):  YE Shao-jing, FANG Ping-chu, MAO Guo-gen, LI Chao-long, Qiu Xiang, CHEN Hai-xiang

Affiliation(s):  Department of Microbiology, College of Medicine, Zhejiang University, Hangzhou 310006, China

Corresponding email(s):   yesj@zju.edu.cn

Key Words:  Helicobacter pylori, CagA, Elution from gel, Western-blot


Share this article to: More

YE Shao-jing, FANG Ping-chu, MAO Guo-gen, LI Chao-long, Qiu Xiang, CHEN Hai-xiang. Purification and relationship with gastric disease of a 130 kDa (CagA) protein of Helicobacter pylori[J]. Journal of Zhejiang University Science A, 2003, 4(2): 232~235.

@article{title="Purification and relationship with gastric disease of a 130 kDa (CagA) protein of Helicobacter pylori",
author="YE Shao-jing, FANG Ping-chu, MAO Guo-gen, LI Chao-long, Qiu Xiang, CHEN Hai-xiang",
journal="Journal of Zhejiang University Science A",
volume="4",
number="2",
pages="232~235",
year="2003",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2003.0232"
}

%0 Journal Article
%T Purification and relationship with gastric disease of a 130 kDa (CagA) protein of Helicobacter pylori
%A YE Shao-jing
%A FANG Ping-chu
%A MAO Guo-gen
%A LI Chao-long
%A Qiu Xiang
%A CHEN Hai-xiang
%J Journal of Zhejiang University SCIENCE A
%V 4
%N 2
%P 232~235
%@ 1869-1951
%D 2003
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2003.0232

TY - JOUR
T1 - Purification and relationship with gastric disease of a 130 kDa (CagA) protein of Helicobacter pylori
A1 - YE Shao-jing
A1 - FANG Ping-chu
A1 - MAO Guo-gen
A1 - LI Chao-long
A1 - Qiu Xiang
A1 - CHEN Hai-xiang
J0 - Journal of Zhejiang University Science A
VL - 4
IS - 2
SP - 232
EP - 235
%@ 1869-1951
Y1 - 2003
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2003.0232


Abstract: 
Objective: The aims of this research were to purify and identify the 130 kDa (cagA) protein of H. pylori clinical isolate HP97002 and evaluate the relationships between the purified 130 kDa (cagA) protein and gastric diseases. Methods: The procedure for isolating the protein included 6 mol/L guanidine extract, size exclusion chromatography and elusion from gel. Sera of 68 patients with gastric diseases (44 with chronic gastritis, 15 with atrophic gastritis, 7 with peptic ulcer disease, 2 with gastric cancer) were obtained, and the serological response to cagA was studied by western-blot using the purified protein. Results: The purified protein was 130 kDa and preserved good antigenicity and revealed basic isoelectric point about of 8.1. Among 68 sera, 43 sera could recognize the purified protein associated with chronic gastritis 47.7% (21/44),atrophic gastritis 86.7% (13/15), peptic ulcer disease 100% (7/7),gastric cancer 100% (2/2). Compared with each other, the difference was significant (χ2=13.327, P=0.004), and 130 kDa (cagA) protein was associated with severe gastric diseases (rs=0.442, P=0.001). Conclusion: The 130 kDa (cagA) protein was associated with severe gastric diseases.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Blaser, M.J., 1992. Hypotheses on the pathogenesis and natural history of Helicobacter pylori-induced inflammation. Gastooenterology, 102: 720-727.

[2]Blaser, M.J., 1997. Heterogeneity of Helicobacter pylori. Eur J Gastroenterol Hepatol(suppl.), 9(1):3-6.

[3]Blum, A.L., 1996. Helicobacter pylori and peptic ulcer disease. Scand J Gastroenterol(suppl.), 31(214): 24-27.

[4]Bridgen, J., 1976. High sensitivity amino acid sequence determination application to proteins eluted from polyacrylamide gels. Biochemistry, 15: 3600-3604.

[5]Bumette, W.H., 1981. Western blotting: electrophoretic transfer of proteins from SDS-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem. 112:195-203.

[6]Crabtree, J.E., 1996. Immune and inflammatory responses to Helicobacter pylori infection. Scand J Gastroenterol(suppl.), 31(215):3-10.

[7]Crabtree, J.E., Taylor, J.D., Wyatt, J.I., Heatley, R.V., Shallcross, T.M. Tompkins, D.S. and Rothbone, B.J.,1991. Mucosal IgA recognition of Helicobacter pylori 128 kDa protein, peptic ulceration, and gastric pathology. Lancet, 338: 332-335.

[8]Covacci, A., Censini, S., Bugnoli, M., Petracca, R., Burroni, D., Macchia, G., Massone, A., Papini, E., Xiang, Z. and Figura, N., 1993. Molecular characterization of the 128kDa immunodominant antigen of Helicobacter pylori associaed with cytotoxicity andduodenal ulcer. Pro Natl Acad Sci USA, 90(6):5791-5795.

[9]Cover, T.L., Dooley, C.P., Blaser, M.J., 1990. Characterization of and human serologic response to proteins in Helicobacter pylori broth culture supernatants with vacuolizing cytotoxin activity. Infect Immun., 58(3): 603-610.

[10]Cover, T.L., Glupczynski, Y., Lage, A.P., Burette, A., Tummuru, M.K., Perez-Perez, G.I. and Blaser, M.J., 1995. Serologic detection of infection with cagA+ Helicobacter pylori steins. J Clin Microbiol., 33(6): 1496-1500.

[11]Fang, P.C., 1993. Study on ECY non-blood medium of Helicobacter pylori. Journal of Zhonghua Medical Detection, 16(3): 131-133(in Chinese).

[12]Leammli, U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227:680-685.

[13]Lee, A., 1996. The nature of Helicobacter pylori. Scand J Gastroenterol, 31(Suppl 214): 5-8.

[14]Megraud, F., 1997. Pathogenic diversity of Helicobacter pylori. J. Gastroenterol, 32(2): 278-281.

[15]Sozzi, M., Valentini, M., Figura, N., De-Paoli, P., Tedeschi, R.M., Gloghini, A., Serraino, D., Poletti, M. and Carbone, A., 1998. Atrophic gastritis and intestinal metaplasia in Helicobacter pylori infection: the role of CagA status. Am J Gastroenterol, 93(3): 375-379.

[16]Telford, J.L., Ghiara, P., Dell'Orco, M., Comanducci, M., Burroni, D., Bugnoli, M., Tecce, M.F., Censini, S., Covacci, A. and Xiang, Z., 1994. Gene structure of the Helicobacter pylori cytotoxin and evicence of its key role in gastric disease. J Exp Med, 179: 1653-1658.

[17]van der Ende, A., Rauws, E.A., Feller, M., Mulder, C.J., Tytgat, G.N. and Dankert, J., 1996. Heterogeneous Helicobacter pylori isolates from members of a family with a history of peptic ulcer disease. Gastroenterology, 111(3): 638-647.

[18]Ye, S.J., Fang, P.C., Li, C.L., 1999. Purification of the 130 kilodalton antigen (CagA) from Helicobacter pylori. Biochemical Research and Application, 4(1): 30-33 (in Chinese).

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE