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Received: 2002-06-26

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Journal of Zhejiang University SCIENCE A 2003 Vol.4 No.3 P.331~335


Cloning and characterization of a glucose 6-phosphate/phosphate translocator from Oryza sativa

Author(s):  JIANG Hua-wu, DIAN Wei-min, LIU Fei-yan, WU Ping

Affiliation(s):  The State Key Laboratory of Plant Physiology and Biochemistry, College of Life sciences, Zhejiang University, Hangzhou 310029, China

Corresponding email(s):   docpwu@cls.zju.edu.cn

Key Words:  Glucose 6-phosphate/phosphate translocator, Starch synthesis, Rice (Oryza sativa L.)

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JIANG Hua-wu, DIAN Wei-min, LIU Fei-yan, WU Ping. Cloning and characterization of a glucose 6-phosphate/phosphate translocator from Oryza sativa[J]. Journal of Zhejiang University Science A, 2003, 4(3): 331~335.

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publisher="Zhejiang University Press & Springer",

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%DOI 10.1631/jzus.2003.0331

T1 - Cloning and characterization of a glucose 6-phosphate/phosphate translocator from Oryza sativa
A1 - JIANG Hua-wu
A1 - DIAN Wei-min
A1 - LIU Fei-yan
A1 - WU Ping
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2003.0331

Plastids of nongreen tissues import carbon as a source of biosynthetic pathways and energy, and glucose 6-phosphate is the preferred hexose phosphate taken up by nongreen plastids. A cDNA clone encoding glucose 6-phosphate/phosphate translocator (GPT) was isolated from a cDNA library of immature seeds of rice and named as OsGPT. The cDNA has one uninterrupted open reading frame encoding a 42 kDa polypeptide possessing transit peptide consisting of 70 amino acid residues. The OsGPT gene maps on chromosome 8 of rice and is linked to the quantitative trait locus for 1000-grain weight. The expression of OsGPT is mainly restricted to heterotrophic tissues. These results suggest that glucose 6-phosphate imported via GPT can be used for starch biosynthesis in rice nongreen plastids.

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