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Bio-Design and Manufacturing  2022 Vol.5 No.10 P.1239~1244

10.1631/jzus.2004.1239


TGF-β1 treated murine dendritic cells are maturation resistant and down-regulate Toll-like receptor 4 expression


Author(s):  MOU Hai-bo, LIN Mao-fang, CEN Hong, YU Jing, MENG Xiao-jian

Affiliation(s):  Department of Bone Marrow Transplantation Center, First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou 310003, China

Corresponding email(s):   mouzxr@hzcnc.com

Key Words:  Dendritic cells, Transforming growth factor &beta, 1, Toll-like receptor 4


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MOU Hai-bo, LIN Mao-fang, CEN Hong, YU Jing, MENG Xiao-jian. TGF-β1 treated murine dendritic cells are maturation resistant and down-regulate Toll-like receptor 4 expression[J]. Journal of Zhejiang University Science D, 2022, 5(10): 1239~1244.

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journal="Journal of Zhejiang University Science D",
volume="5",
number="10",
pages="1239~1244",
year="2022",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2004.1239"
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%0 Journal Article
%T TGF-β1 treated murine dendritic cells are maturation resistant and down-regulate Toll-like receptor 4 expression
%A MOU Hai-bo
%A LIN Mao-fang
%A CEN Hong
%A YU Jing
%A MENG Xiao-jian
%J Journal of Zhejiang University SCIENCE D
%V 5
%N 10
%P 1239~1244
%@ 1869-1951
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2004.1239

TY - JOUR
T1 - TGF-β1 treated murine dendritic cells are maturation resistant and down-regulate Toll-like receptor 4 expression
A1 - MOU Hai-bo
A1 - LIN Mao-fang
A1 - CEN Hong
A1 - YU Jing
A1 - MENG Xiao-jian
J0 - Journal of Zhejiang University Science D
VL - 5
IS - 10
SP - 1239
EP - 1244
%@ 1869-1951
Y1 - 2022
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2004.1239


Abstract: 
Objective: To explore the effects of transforming growth factor &beta;1 (TGF-β1) on dendritic cells (DC). Methods: Murine bone marrow cells were cultured with GM-CSF and TGF-β1 to develop TGF-β1-treated DC (TGFβ-DC). Then they were stimulated by lipopolysaccharide (LPS). Their phenotypes were assessed by flow cytometry (FCM). The allogeneic stimulating capacity of DC was measured by mixed lymphocyte reaction (MLR) using BrdU ELISA method and IL-12p70 protein was detected by ELISA. The expression of toll-like receptor 4 (TLR4) was analyzed by semi quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and FCM. Results: Compared to immature DC (imDC) cultured by GM-CSF alone, the TGFβ-DC express lower CD80, CD86, I-Ab and CD40. The TGFβ-DC were resistant to maturation with LPS. Maturation resistance was evident from a failure to up-regulate co-stimulatory molecules (CMs), to stimulate larger T cells proliferation and to enhance secretion of IL-12p70. We also found that TGF-β1 could down-regulate TLR4 expression on TGFβ-DC. Conclusion: TGFβ-DC are resistant to maturation stimulus (LPS) and might have some correlation with the down-modulation of TLR4 expression.

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