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Journal of Zhejiang University SCIENCE A 2004 Vol.5 No.12 P.1583-1589


Effect of temperature on batch elastase production by Bacillus sp. EL31410

Author(s):  HE Guo-qing, XU Ying, CHEN Qi-he, RUAN Hui, LI Jing-jun

Affiliation(s):  Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   gqhe@zju.edu.cn

Key Words:  Bacillus sp. EL31410, Elastase, Temperature, Batch fermentation, Temperature-shift strategy

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HE Guo-qing, XU Ying, CHEN Qi-he, RUAN Hui, LI Jing-jun. Effect of temperature on batch elastase production by Bacillus sp. EL31410[J]. Journal of Zhejiang University Science A, 2004, 5(12): 1583-1589.

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publisher="Zhejiang University Press & Springer",

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%A XU Ying
%A CHEN Qi-he
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%DOI 10.1631/jzus.2004.1583

T1 - Effect of temperature on batch elastase production by Bacillus sp. EL31410
A1 - HE Guo-qing
A1 - XU Ying
A1 - CHEN Qi-he
A1 - RUAN Hui
A1 - LI Jing-jun
J0 - Journal of Zhejiang University Science A
VL - 5
IS - 12
SP - 1583
EP - 1589
%@ 1869-1951
Y1 - 2004
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2004.1583

The production of elastase by bacillus sp. EL31410 at various temperatures was investigated. In order to study the effect of temperature on elastase fermentation, different cultivation temperatures, ranging from 39 °C to 28 °C, were evaluated in shake flask. The result indicated that 37 °C was best for cell growth at earlier stage; while maximum elastase activity was obtained when the cells were cultivated at 30 °C. This result was verified by batch fermentation in 5-L bioreactor under 37 °C and 30 °C temperature, respectively. The specific cell growth rate at 37 °C was higher than that at 30 °C during earlier stage of cultivation. The maximum value [5.5 U/(h·g DCW)] of elastase formation rate occurred at 24 h at 30 °C compared to 4.6 U/(h·g DCW) at 30 h at 37 °C. Based on these results, two-stage temperature shift strategy and oscillatory temperature cultivation mode were evaluated in the next study. When compared to single temperature of 37 °C or 30 °C, both two-stage temperature shift strategy and oscillatory temperature strategy improved biomass but did not yield the same result as expected for elastase production. The maximum biomass (both 8.6 g/L) was achieved at 30 h at 37 °C, but at 42 h using two-stage temperature cultivation strategy. The highest elastase production (652 U/ml) was observed at 30 °C in batch process. It was concluded that cultivation at constant temperature of 30 °C was appropriate for elastase production by bacillus sp. EL31410.

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