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Journal of Zhejiang University SCIENCE A 2004 Vol.5 No.12 P.1583-1589

http://doi.org/10.1631/jzus.2004.1583


Effect of temperature on batch elastase production by Bacillus sp. EL31410


Author(s):  HE Guo-qing, XU Ying, CHEN Qi-he, RUAN Hui, LI Jing-jun

Affiliation(s):  Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   gqhe@zju.edu.cn

Key Words:  Bacillus sp. EL31410, Elastase, Temperature, Batch fermentation, Temperature-shift strategy


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HE Guo-qing, XU Ying, CHEN Qi-he, RUAN Hui, LI Jing-jun. Effect of temperature on batch elastase production by Bacillus sp. EL31410[J]. Journal of Zhejiang University Science A, 2004, 5(12): 1583-1589.

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%A CHEN Qi-he
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T1 - Effect of temperature on batch elastase production by Bacillus sp. EL31410
A1 - HE Guo-qing
A1 - XU Ying
A1 - CHEN Qi-he
A1 - RUAN Hui
A1 - LI Jing-jun
J0 - Journal of Zhejiang University Science A
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EP - 1589
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2004.1583


Abstract: 
The production of elastase by bacillus sp. EL31410 at various temperatures was investigated. In order to study the effect of temperature on elastase fermentation, different cultivation temperatures, ranging from 39 °C to 28 °C, were evaluated in shake flask. The result indicated that 37 °C was best for cell growth at earlier stage; while maximum elastase activity was obtained when the cells were cultivated at 30 °C. This result was verified by batch fermentation in 5-L bioreactor under 37 °C and 30 °C temperature, respectively. The specific cell growth rate at 37 °C was higher than that at 30 °C during earlier stage of cultivation. The maximum value [5.5 U/(h·g DCW)] of elastase formation rate occurred at 24 h at 30 °C compared to 4.6 U/(h·g DCW) at 30 h at 37 °C. Based on these results, two-stage temperature shift strategy and oscillatory temperature cultivation mode were evaluated in the next study. When compared to single temperature of 37 °C or 30 °C, both two-stage temperature shift strategy and oscillatory temperature strategy improved biomass but did not yield the same result as expected for elastase production. The maximum biomass (both 8.6 g/L) was achieved at 30 h at 37 °C, but at 42 h using two-stage temperature cultivation strategy. The highest elastase production (652 U/ml) was observed at 30 °C in batch process. It was concluded that cultivation at constant temperature of 30 °C was appropriate for elastase production by bacillus sp. EL31410.

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