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Journal of Zhejiang University SCIENCE A 2004 Vol.5 No.12 P.1608~1612


Characterization of cellulose acetate micropore membrane immobilized acylase I

Author(s):  GUO Yong-sheng, WANG Jie, SONG Xi-jin

Affiliation(s):  Department of Chemistry, Zhejiang University, Hangzhou 310027, China

Corresponding email(s):   wjjw@zju.edu.cn

Key Words:  Acylase I, CA-CTA micropore membrane, Enzyme immobilization, Orthogonal experiment

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GUO Yong-sheng, WANG Jie, SONG Xi-jin. Characterization of cellulose acetate micropore membrane immobilized acylase I[J]. Journal of Zhejiang University Science A, 2004, 5(12): 1608~1612.

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author="GUO Yong-sheng, WANG Jie, SONG Xi-jin",
journal="Journal of Zhejiang University Science A",
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%A SONG Xi-jin
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%DOI 10.1631/jzus.2004.1608

T1 - Characterization of cellulose acetate micropore membrane immobilized acylase I
A1 - GUO Yong-sheng
A1 - WANG Jie
A1 - SONG Xi-jin
J0 - Journal of Zhejiang University Science A
VL - 5
IS - 12
SP - 1608
EP - 1612
%@ 1869-1951
Y1 - 2004
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2004.1608

This paper describes an innovative method for the immobilization of acylase I, which was entrapped into the CA-CTA micropore membrane. The most suitable casting solutions proportion for immobilizing the enzyme was obtained through orthogonal experiment. Properties of the enzyme membrane were investigated and compared with those of free enzyme and blank membrane. The thermal stability and pH stability of the enzyme inside the membrane were changed by immobilization. The optimum pH was found to be 6.0, which changes 1.0 unit compared with that of free acylase I. The optimum temperature was found to be about 90 °C, which is higher than that of free acylase I (60 °C). Experimental results showed that immobilization had effects on the kinetic parameters of acylase I.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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Open peer comments: Debate/Discuss/Question/Opinion



2014-11-25 04:17:44

I'd like to read this article. Thank you!

vishal@No address<vishalgaikwad400@gmail.com>

2013-07-16 14:41:44

Ill like to read this article because im interested on the enzyme immobilization methods.


2010-05-28 20:23:36

i would like to know whether immobilization brought about any change in the catalytic activity of the enzyme


2010-05-25 10:07:10

Ill like to read this article, because im interested on the enzyme immobilization methods.


2010-05-25 09:59:01

I like to read this

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