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Journal of Zhejiang University SCIENCE A 2004 Vol.5 No.12 P.1608~1612

http://doi.org/10.1631/jzus.2004.1608


Characterization of cellulose acetate micropore membrane immobilized acylase I


Author(s):  GUO Yong-sheng, WANG Jie, SONG Xi-jin

Affiliation(s):  Department of Chemistry, Zhejiang University, Hangzhou 310027, China

Corresponding email(s):   wjjw@zju.edu.cn

Key Words:  Acylase I, CA-CTA micropore membrane, Enzyme immobilization, Orthogonal experiment


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GUO Yong-sheng, WANG Jie, SONG Xi-jin. Characterization of cellulose acetate micropore membrane immobilized acylase I[J]. Journal of Zhejiang University Science A, 2004, 5(12): 1608~1612.

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T1 - Characterization of cellulose acetate micropore membrane immobilized acylase I
A1 - GUO Yong-sheng
A1 - WANG Jie
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J0 - Journal of Zhejiang University Science A
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2004.1608


Abstract: 
This paper describes an innovative method for the immobilization of acylase I, which was entrapped into the CA-CTA micropore membrane. The most suitable casting solutions proportion for immobilizing the enzyme was obtained through orthogonal experiment. Properties of the enzyme membrane were investigated and compared with those of free enzyme and blank membrane. The thermal stability and pH stability of the enzyme inside the membrane were changed by immobilization. The optimum pH was found to be 6.0, which changes 1.0 unit compared with that of free acylase I. The optimum temperature was found to be about 90 °C, which is higher than that of free acylase I (60 °C). Experimental results showed that immobilization had effects on the kinetic parameters of acylase I.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1] Baran, T., Arica, M.Y., Denizli, A., 1997. Comparison of β-galactosidase immobilization by entrapment in and adsorption on poly (2-hydroxyethylmethacrylate) membranes. Polym. Int., 44:530-536.

[2] Caras, S., Janata, J., 1980. Field effect transitor sensitive to penicillin. Anal. Chem., 52:1935-1937.

[3] Chen, J., Sun, Y., Chu, D., 1998. Immobilization of α-amylase to a composite temperature-sensitive membrane for starch hydrolysis. Biotechnol. Prog., 14:473-478.

[4] Deniz, T., Belma, I.Y., Ahmet, R.O., 1998. Immobilization of a thermostable α-amylase, Termamyl®, onto nitrocellulose membrane by Cibacron Blue F3GA dye binding. Biochem. Eng., 2(3):179-186.

[5] Guo, Y.S., Wang, J., Dong, J., Song, X.J., 2001. Study on immobilization of acylase with cellulose acetate membrane. Prog. Biochem. Biophys., 28(5):748-751 (in Chinese).

[6] Jiang, X.R., Yang, J., Ren, K.Q., 1999. Production of 7-amidocephalosporanic acid from glutaryl-7-amidocephalosporanic acid by immobilized recombinant Escherichia coli cells. Zhongguo Kangshengsu Zazhi, 24(4):273-276 (in Chinese).

[7] Ling, D., Wu, G., Wang, C., Wang, F., Song, G., 2000. The preparation and characterization of an immobilized L-glutamic decarboxylase and its application for determination of L-glutamic acid. Enzyme and Microbial Technol., 27(7):516-521.

[8] Shen, S.Y., Tu, S.I., 1999. Immobilization of horseradish peroxidase in cross-linked phyllosilicates: conditions and characterizations. Biotechnol. Appl. Biochem., 29:185-189.

[9] Shin, I.S., Yu, J.K., Akira, I., 1998. Immobilization of glucose oxidase on poly-(L-lysine)-modified polycarbonate membrane. Biotechnol. Appl. Biochem., 27:245-248.

[10] Wang, J., Song, X.J., Wu, M., 1998. Studies on immobilization of urease on cellulose acetate membrane. Chem. J. Chinese Universities, 19(7):1104-1106 (in Chinese).

[11] Zhang, L.X., Zhang, T.F., Li, L.Y., 1981. Method and Technology of Biochemical Experiment. Higher Education Press, Beijing, p.155 (in Chinese).

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Yue@UTA<lyly1990@utexas.edu>

2014-11-25 04:17:44

I'd like to read this article. Thank you!

vishal@No address<vishalgaikwad400@gmail.com>

2013-07-16 14:41:44

Ill like to read this article because im interested on the enzyme immobilization methods.

nima<pravijak@gmail.com>

2010-05-28 20:23:36

i would like to know whether immobilization brought about any change in the catalytic activity of the enzyme

Hiram@UAdeC<Orionorbital@hotmail.com>

2010-05-25 10:07:10

Ill like to read this article, because im interested on the enzyme immobilization methods.

Hiram<Orionorbital@hotmail.com>

2010-05-25 09:59:01

I like to read this

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