Full Text:   <1258>

CLC number: TH742.1

On-line Access: 

Received: 2005-01-28

Revision Accepted: 2005-04-05

Crosschecked: 0000-00-00

Cited: 0

Clicked: 3506

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE A 2005 Vol.6 No.7 P.623~626


Improving the sensitivity of protein microarray by evanescent-field-induced fluorescence

Author(s):  WANG Li-qiang, LU Zu-kang

Affiliation(s):  State Key Lab of Modern Optical Instrumentation, Zhejiang University, Hangzhou 310027, China

Corresponding email(s):   wangliqiang@zju.edu.cn

Key Words:  Protein microarray, Evanescent excitation, Sensitivity, Total reflection

WANG Li-qiang, LU Zu-kang. Improving the sensitivity of protein microarray by evanescent-field-induced fluorescence[J]. Journal of Zhejiang University Science A, 2005, 6(7): 623~626.

@article{title="Improving the sensitivity of protein microarray by evanescent-field-induced fluorescence",
author="WANG Li-qiang, LU Zu-kang",
journal="Journal of Zhejiang University Science A",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Improving the sensitivity of protein microarray by evanescent-field-induced fluorescence
%A WANG Li-qiang
%A LU Zu-kang
%J Journal of Zhejiang University SCIENCE A
%V 6
%N 7
%P 623~626
%@ 1673-565X
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.A0623

T1 - Improving the sensitivity of protein microarray by evanescent-field-induced fluorescence
A1 - WANG Li-qiang
A1 - LU Zu-kang
J0 - Journal of Zhejiang University Science A
VL - 6
IS - 7
SP - 623
EP - 626
%@ 1673-565X
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.A0623

To improve the sensitivity of protein microarray, a prism surface replaces the surface of the common microscope slide. The protein targets arrayed on the surface are hybridized and labelled by fluorescent probes. evanescent excitation occurs when the convergent laser reaches the surface, and a photomultiplier tube detects the emitted fluorescent signal. A two-dimensional actuator scans the whole surface to achieve planar laser excitation and fluorescence collection. The penetration depth of the evanescent field into the protein targets is only some hundred nanometers and can be controlled by different incident angle of the laser beam, so the undesired background signals are reduced dramatically and the detection sensitivity is improved by a factor of 50 to 100 comparing to confocal excitation. This approach can detect low abundance analytes without signal amplification.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Duveneck, G.L., Abel, A.P., Bopp, M.A., Kresbach, G.M., Ehrat, M., 2002. Planar waveguides for ultra-high sensitivity of the analysis of nucleic acids. Analytica Chimica. Acta, 469(1):49-61.

[2] Miklos, G.L., Maleszka, R., 2001. Protein functions and biological contexts. Proteomics, 1(2):169-178.

[3] Pawlak, M., Schick, E., Bopp, M.A., Schneider, M.J., Oroszlan, P., Ehrat, M., 2002. Zeptosens’ protein microarrays: a novel high performance microarray platform for low abundance protein analysis. Proteomics, 2(4):383-393.

[4] Rachlin, D.J., 2002. Optimized approach for microarray scanning. SPIE, 4623:13-26.

[5] Schena, M., 1999. DNA Microarrays−A Practical Approach. Oxford University Press, New York, p.17-42.

[6] Toomre, D., Manstein, D.J., 2001. Lighting up the cell surface with evanescent wave microscopy. TRENDS in Cell Biology, 11(7):298-302.

[7] Wang, L., Ni, X., Lu, Z., Zheng, X., Li, Y., 2004. Enhancing the quality metric of protein microarray image. Journal of Zhejiang University SCIENCE, 5(12):1621-1628.

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE