Full Text:   <1529>

CLC number: R692.31

On-line Access: 

Received: 2004-03-26

Revision Accepted: 2004-04-24

Crosschecked: 0000-00-00

Cited: 4

Clicked: 4378

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.1 P.61~68

http://doi.org/10.1631/jzus.2005.B0061


Oxidative stress and damage induced by abnormal free radical reactions and IgA nephropathy


Author(s):  CHEN Jia-xi, ZHOU Jun-fu, SHEN Han-chao

Affiliation(s):  Division of Nephrology, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China

Corresponding email(s):   jiaxi9@mail.hz.zj.cn

Key Words:  Chronic glomerulonephritis, Free radicals, Oxidation, Lipoperoxidation, Nitric oxide, Lipoperoxide, Antioxidant, Antioxidase, Oxidative stress, Oxidative damage


CHEN Jia-xi, ZHOU Jun-fu, SHEN Han-chao. Oxidative stress and damage induced by abnormal free radical reactions and IgA nephropathy[J]. Journal of Zhejiang University Science B, 2005, 6(1): 61~68.

@article{title="Oxidative stress and damage induced by abnormal free radical reactions and IgA nephropathy",
author="CHEN Jia-xi, ZHOU Jun-fu, SHEN Han-chao",
journal="Journal of Zhejiang University Science B",
volume="6",
number="1",
pages="61~68",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B0061"
}

%0 Journal Article
%T Oxidative stress and damage induced by abnormal free radical reactions and IgA nephropathy
%A CHEN Jia-xi
%A ZHOU Jun-fu
%A SHEN Han-chao
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 1
%P 61~68
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0061

TY - JOUR
T1 - Oxidative stress and damage induced by abnormal free radical reactions and IgA nephropathy
A1 - CHEN Jia-xi
A1 - ZHOU Jun-fu
A1 - SHEN Han-chao
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 1
SP - 61
EP - 68
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0061


Abstract: 
Objective: To estimate the oxidative stress and oxidative damage induced by abnormal free radical reactions in IgA nephropathy (IgAN) patients’ bodies. Methods: Seventy-two IgA N patients (IgANP) and 72 healthy adult volunteers (HAV) were enrolled in a random control study design, in which the levels of nitric oxide (NO) in plasma, lipoperoxide (LPO) in plasma and in erythrocytes, and vitamin C (VC), vitamin E (VE) and β-carotene (β-CAR) in plasma as well as the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) in erythrocytes were determined with spectrophotometric mothods. Results: Compared with the HAV group, the averages of NO in plasma, and LPO in plasma and in erythrocytes in the IgANP group were significantly increased (P<0.0001), while those of VC, VE and β-CAR in plasma as well as those of SOD, CAT and GPX in erythrocytes in the IgANP group were significantly decreased (P<0.0001). Linear correlation analysis showed that with the increase of the values of NO, and LPO in plasma and in erythrocytes, and with the decrease of those of VC, VE, β-CAR, SOD, CAT and GPX in the IgAN patients, the degree of histological damage of tubulointerstitial regions was increased gradually (P<0.0001); and that with the prolongation of the duration of disease the values of NO, and LPO in plasma and erythrocytes were increased gradually, while those of VC, VE, β-CAR, SOD, CAT and GPX were decreased gradually (P<0.005). The discriminatory correct rates of the above biochemical parameters reflecting oxidative damage of the IgAN patients were 73.8%-92.5%, and the correct rates for the HAV were 70.0%-91.3% when independent discriminant analysis was used; and the correct rate for the IgAN patients was increased to 98.8%, the correct rate for the HAV was increased to 100% when stepwise discriminant analysis was used. The above biochemical parameters’ reliability coefficient (alpha) were used to estimate the oxidative damage of the IgAN patients as 0.8145, the standardized item alpha=0.9730, F=53273.5681, P<0.0001. Conclusions: A series of free radical chain reactions caused serious pathological aggravation in the IgANP’ bodies, thus resulting in oxidative damage in their bodies. In treating IgANP, therefore, it is necessary that suitable dose antioxidants should be supplemented to them so as to alleviate the oxidative damage in their bodies.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1] Appel, G.B., 1996. Cecil Textbook of Medicine. In: Bennett, J.C., Plum, F. (Eds.), Glomerular Disorders. WB Saunders Company, Philadelphia, p.552-559.

[2] Endreffy, E., Turi, S., Laszik, Z., Bereczki, C., Kasa, K., 1991. The effects of vitamin E on tissue oxidation in nephrotoxic (anti-glomerular basement membrane) nephritis. Pediatr Nephrol, 5(3):312-317.

[3] Girotti, A.W., 1998. Lipid hydroperoxide generation turnover and effector action in biological systems. J Lipid Res, 39(8):1529-1542.

[4] Goldstein, R.S., Schnellmann, R.G., 1998. Casarett and Doull’s Toxicology–The Basic Science of Poisons. In: Klaassen, C.D., Amdur, M.O., Doull, J. (Eds.), Toxic Responses of the Kidney. McGraw-Hill Companies Inc., New York, p.417-442.

[5] Kakoki, M., Matsumoto, A., Nagata, D., Kobayakawa, N., Kimura, K., Momomura, S., Hirata, Y., 1999. Analysis of nitric oxide in the exhaled air of patients with chronic glomerulonephritis. Clin Nephrol, 52(2):83-90.

[6] Kasiske, B.L., Keane, W.F., 1999. Brenner & Rector’s The Kidney. In: Brenner, B.M. (Ed.), Laboratory Assessment of Renal Disease: Clearance, Urinalysis, and Renal Biopsy. Harcourt Asia Pte Ltd., Singapore, p.1127-1130.

[7] Lang, T.A., Secic, M., 1997. How to Report Statistics in Medicine. Port City Press, Philadelphia, p.65-80.

[8] Liu, Z.H., Li, L.S., 1992. Renal interstitial damade in chronic glomerulonephritis. Chinese Journal of Internal Medicine, 31:52-54 (in Chinese).

[9] Mayes, P.A., 2000. Harper’s Biochemistry. In: Murray, R.K., Granner, D.K., Mayes, P.A. (Eds.), Biologic Oxidation. McGraw-Hill Companies Inc., New York, p.130-136.

[10] McCarthy, E.T., Sharma, R., Sharma, M., Li, J.Z., Ge, X.L., Dileezpan, K.N., Savin, V.J., 1998. TNF-alpha increases albumin permeability of isolated rat glomeruli through the generation of superoxide. J Am Soc Nephrol, 9(3):433-438.

[11] McKee, T., McKee, J.R., 2000. Biochemistry: An Introduction. McGraw-Hill Companies Inc., New York, p.289-330.

[12] Shah, S.V., 1991. Oxidant mechanisms in glomerulonephritis. Semin Nephrol, 11(3):320-326.

[13] Sharma, R., Khanna, A., Sharma, M., Savin, V.J., 2000. Transforming growth factor-beta1 increases albumin permeability of isolated rat glomeruli via hydroxyl radicals. Kidney Int, 58(1):131-136.

[14] Templar, J., Kon, S.P., Milligan, T.P., Newman, D.J., Raftery, M.J., 1999. Increased plasma malondialdehyde levels in glomerular disease as determined by a fully validated HPLC method. Nephrol Dial Transplant, 14(4):946-951.

[15] Trachtman, H., Chan, J.C., Chan, W., Valderrama, E., Brandt, R., Wakely, P., Futterweit, S., Maesaka, J., Ma, C., 1996. Vitamin E ameliorates renal injury in an experimental model of immunoglobulin A nephropathy. Pediatr Res, 40(4):620-626.

[16] Zhou, J.F., Yan, X.F., Guo, F.Z., Sun, N.Y., Qian, Z.J., Ding, D.Y., 2000. Effects of cigarette smoking and smoking cessation on plasma constituents and enzyme activities related to oxidative stress. Biomed Environ Sci, 13(1):44-55.

[17] Zima, T., Stipek, S., Crkovska, J., Nemecek, K., Fialova, J., Platenik, J., Bartova, V., 1996. Lipid peroxidation and antioxidant enzymes in CAPD patients. Ren Fail, 18(1):113-119.

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE