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Received: 2004-05-13

Revision Accepted: 2004-10-28

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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.2 P.142~146


Isolation and characterization of a novel strain of Natrinema containing a bop gene

Author(s):  XU Xue-wei, WU Min, HUANG Wei-da

Affiliation(s):  School of Life Sciences, Zhejiang University, Hangzhou 310027, China; more

Corresponding email(s):   wumin@cls.zju.edu.cn

Key Words:  Halophilic archaea, Phylogeny, Bacteriorhodopsin

XU Xue-wei, WU Min, HUANG Wei-da. Isolation and characterization of a novel strain of Natrinema containing a bop gene[J]. Journal of Zhejiang University Science B, 2005, 6(2): 142~146.

@article{title="Isolation and characterization of a novel strain of Natrinema containing a bop gene",
author="XU Xue-wei, WU Min, HUANG Wei-da",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Isolation and characterization of a novel strain of Natrinema containing a bop gene
%A XU Xue-wei
%A WU Min
%A HUANG Wei-da
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 2
%P 142~146
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0142

T1 - Isolation and characterization of a novel strain of Natrinema containing a bop gene
A1 - XU Xue-wei
A1 - WU Min
A1 - HUANG Wei-da
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 2
SP - 142
EP - 146
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0142

A novel member of extremely halophilic archaea, strain AJ2, was isolated from Ayakekum Lake located in Altun Mountain National Nature Reserve of Xinjiang Uygur Autonomous Region in China. The strain AJ2 requires at least 10% (w/v) NaCl and grows 10% to 30% (optimum at 20%). Phylogenetic analysis based on 16S rDNA sequence comparison revealed that strain AJ2 clustered to three Natrinema species with less than 97.7% sequence similarities, suggesting AJ2 is a novel member of Natrinema. A bacteriorhodopsin-encoding (bop) gene was subsequently detected in the AJ2 genome using the polymerase chain reaction technique. The cloning and sequencing of a 401 base pairs fragment indicated the deduced amino acid sequence of bop from AJ2 is different from that reported for bacteriorhodopsins. This is the first reported detection of a bop gene in Natrinema.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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