Full Text:   <1377>

CLC number: X53

On-line Access: 

Received: 2005-01-16

Revision Accepted: 2005-04-16

Crosschecked: 0000-00-00

Cited: 12

Clicked: 4451

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.6 P.546~552


Changes of root morphology and Pb uptake by two species of Elsholtzia under Pb toxicity

Author(s):  PENG Hong-yun, TIAN Sheng-ke, YANG Xiao-e

Affiliation(s):  Ministry of Education Key Lab of Environment, Remediation and Ecosystem Health, School of Natural Resources and Environmental Science, Zhejiang University, Hangzhou 310029, China

Corresponding email(s):   penghongyun@zju.edu.cn, xyang@zju.edu.cn

Key Words:  EDTA, Elsholtzia, Pb, Phytoremedation, Root morphology

PENG Hong-yun, TIAN Sheng-ke, YANG Xiao-e. Changes of root morphology and Pb uptake by two species of Elsholtzia under Pb toxicity[J]. Journal of Zhejiang University Science B, 2005, 6(6): 546~552.

@article{title="Changes of root morphology and Pb uptake by two species of Elsholtzia under Pb toxicity",
author="PENG Hong-yun, TIAN Sheng-ke, YANG Xiao-e",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Changes of root morphology and Pb uptake by two species of Elsholtzia under Pb toxicity
%A PENG Hong-yun
%A TIAN Sheng-ke
%A YANG Xiao-e
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 6
%P 546~552
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0546

T1 - Changes of root morphology and Pb uptake by two species of Elsholtzia under Pb toxicity
A1 - PENG Hong-yun
A1 - TIAN Sheng-ke
A1 - YANG Xiao-e
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 6
SP - 546
EP - 552
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0546

Elsholtzia argyi and Elsholtzia splendens, which are Chinese endemic pb/Zn mined and Cu mined ecotype respectively, were investigated in the aspect of their response to pb toxicity in the presence or absence of EDTA addition. After 8 d’s pb treatment, root length, root surface area and root volume of E. splendens decreased much more than those of E. argyi, and reduced considerably with increase of pb, while no marked change was noted for root average diameter. Compared to E. argyi, length of root with diameter (D)<0.2 mm was significantly reduced for E. splendens as pb increasing. Root with cross-sectional area of D<0.1 mm for E. splendens was at pb≥10 mg/L, while for E. argyi, it was at pb≥25 mg/L. DW of E. splendens decreased much more than that of E. argyi with increase of pb. E. argyi exhibited much more tolerance to pb toxicity than E. splendens. Treatment with 100 mg/L pb plus 50 mmol/L EDTA significantly decreased the length and surface area of D≤0.2 mm root, increased the length and surface area of 0.2≤D≤0.8 mm root for the case of E. argyi, while for E. splendens, length and surface area of D<0.6 mm root reduced, as compared to 100 mg/L pb treatment, alone. At 100 mg/L pb, shoot pb accumulation in E. splendens and E. argyi were 27.9 and 89.0 μg/plant DW respectively, and much more pb was uptaken by the root and translocated to the stem of E. argyi as compared to E. splendens. Treatment of the plant with 100 mg/L pb plus 50 mmol/L EDTA increase leaf pb accumulation from 16.8 to 84.9 g/plant for E.splendens and from 18.8 to 52.5 g/plant for E. argyi, while both root and stem pb pronouncedly reduced for both Elsholtzia species. The increased translocation of pb to the leaf of E. splendens than that of E. argyi at the treatment of 100 mg/L pb plus 50 mmol/L EDTA should be further investigated.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Baker, A.J.M., Brooks, R.R., 1989. Terrestrial higher plants which hyperaccumulate metallic elements: A review of their distribution, ecology and photochemistry. Biorecovery, 1:81-126.

[2] Blaylock, M., Salt, D.E., Dushenkov, S., Zakharova, O., Gussman, C., Kapulnik, Y., Ensley, B.D., Raskin, I., 1997. Enhanced accumulation of Pb in Indian Mustard by soil-applied chelating agents. Environmental Science and Technology, 31:860-865.

[3] Huang, J.W., Cunningham, S.D., 1996. Lead phytoextraction: Species variation in lead uptake and translocation. New Phytologist, 134:75-84.

[4] Jarvis, M.D., Leung, D.W.M., 2001. Chelated lead transport in Chamaecytisus proliferus (L.f.) link ssp. proliferus var. palmensis (H. Christ): An ultrastructural study. Plant Science, 161:433-441.

[5] Marschner, H., 1995. Mineral Nutrition of Higher Plants. 2nd Ed., Academic Press, San Diego, CA, USA.

[6] Peng, H.Y., Yang, X.E., 2005. Volatile constituents in the flowers of Elsholtzia argyi and their variation−a possible utilization of plant resources after phytoremediation. Journal of Zhejiang University SCIENCE, 6B(2):91-95.

[7] Raskin, I., Smith, R.D., Salt, D.E., 1997. Phytoremediation of metals: Using plants to remove pollutants from the environment. Current Opinion in Biotechnology, 8:221-226.

[8] Reeves, R.D., Brooks, R.R., 1983. Hyperaccumulation of lead and zinc by two metallophytes from a mine area in Central Europe. Environmental Pollution Ser A, 31:275-283.

[9] Salt, D.E., Michael, B., Nanda, P.B.A.K., Viatcheslav, D., Burt, D.E., Ilan, C., 1995. Phytoremediation: A novel strategy for the removal of toxic metals from the environment using plants. Biotechnology, 13:468-474.

[10] Scott, D.C., David, W.O., 1996. Promises and prospects of phytoremediation. Plant Physiology, 110:715-719.

[11] Song, J., Zhao, F.J., Luo, Y.M., McGrath, S.P., Zhang, H., 2004. Copper uptake by Elsholtzia splendens and Silene vulgaris and assessment of copper phytoavailability in contaminated soils. Environmental Pollution, 128:307-315.

[12] Yang, X.E., Shi, W.Y., Fu, C.X., Yang, M.J., 1998. Copper-Hyperaccumulators of Chinese Native Plants, Characteristics and Possible Use for Phytoremediation. Sustainable Agriculture for Food, Energy and Industry, James & James (Science Publishers) Ltd.

Open peer comments: Debate/Discuss/Question/Opinion



2010-10-26 19:42:52


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE