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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.7 P.622~625

http://doi.org/10.1631/jzus.2005.B0622


Effects of fructose-1,6-diphosphate on concentration of calcium and activities of sarcoplosnic Ca2+-ATPase in cardiomyocytes of Adriamycin-treated rats


Author(s):  CAI Wei, CHEN Jun-zhu, RUAN Li-ming, WANG Yi-na

Affiliation(s):  Department of Internal Medicine, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China

Corresponding email(s):   weic236@sohu.com

Key Words:  Fructose-1, 6-diphosphate, Adriamycin, Cardiomyocyte, Calcium, Sarcoplosnic reticulum Ca2+-ATPase


CAI Wei, CHEN Jun-zhu, RUAN Li-ming, WANG Yi-na. Effects of fructose-1,6-diphosphate on concentration of calcium and activities of sarcoplosnic Ca2+-ATPase in cardiomyocytes of Adriamycin-treated rats[J]. Journal of Zhejiang University Science B, 2005, 6(7): 622~625.

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author="CAI Wei, CHEN Jun-zhu, RUAN Li-ming, WANG Yi-na",
journal="Journal of Zhejiang University Science B",
volume="6",
number="7",
pages="622~625",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B0622"
}

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%T Effects of fructose-1,6-diphosphate on concentration of calcium and activities of sarcoplosnic Ca2+-ATPase in cardiomyocytes of Adriamycin-treated rats
%A CAI Wei
%A CHEN Jun-zhu
%A RUAN Li-ming
%A WANG Yi-na
%J Journal of Zhejiang University SCIENCE B
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%N 7
%P 622~625
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0622

TY - JOUR
T1 - Effects of fructose-1,6-diphosphate on concentration of calcium and activities of sarcoplosnic Ca2+-ATPase in cardiomyocytes of Adriamycin-treated rats
A1 - CAI Wei
A1 - CHEN Jun-zhu
A1 - RUAN Li-ming
A1 - WANG Yi-na
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 7
SP - 622
EP - 625
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0622


Abstract: 
Objective: To observe the effects of fructose-1,6-diphosphate (FDP) on serum levels of cardiac troponin I (cTnI) and creatine kinase-MB (CK-MB), as well as the concentration of calcium in cardiomyocytes (Myo[Ca2+]) and activity of sarcoplosnic Ca2+-ATPase (SRCa2+-ATPase) in adriamycin (ADR)-treated rats. Methods: Rats were intraperitoneally injected with ADR (2.5 mg/kg every other day for 6 times) and then with different dosages of FDP (every other day for twenty-one times). Bi-antibodies sandwich Enzyme linked immune absorption assay (ELISA) was performed to detect serum level of cTnI. CK-MB was detected by monoclonal antibody, Myo[Ca2+] was detected by fluorescent spectrophotometry and the activity of SRCa2+-ATPase was detected by inorganic phosphate method. Results: FDP (300, 600, 1200 mg/kg) significantly reduced the serum levels of cTnI and CK-MB, while at the same time decreased calcium concentration and increased SRCa2+-ATPase activity in cardiomyocytes of ADR-treated rats (P<0.01). Conclusions: FDP might alleviate the cardiotoxic effects induced by ADR through decreasing calcium level as well as increasing SRCa2+-ATPase activity in cardiomyocytes.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1] Bickler, P.E., Kellecher, J.A., 1992. Fructose-1,6-diphosphate stabilizes brain intracellular calcium during hypoxia in rats. Stroke, 23:1617-1622.

[2] Galzigna, L., Rizzoli, V., Bianchi, M., Rigobello, M.P., Scuri, R., 1989. Some effects of fructose-1,6-diphosphate on rat myocardial tissue related to amembeane-stabilixing action. Cell Biochem Function, 7:91-96.

[3] Hua, D., Zhuang, X., Ye, J., Wilson, D., Chiang, B., Chien, S., 2003. Using fructose-1,6-diphosphate during hypothermic rabbit-heart preservation: A high-energy phosphate study. J Heart Lung Transplant, 22:574-582.

[4] Huang, X.M., Zhu, W.H., Kang, M.L., 2003. Study on the effect of doxorubicin on expressions of genes encoding myocardiac sarcoplasmic reticulum Ca2+ transport myocardiac protection in rabbits. J Zhejiang Univ Sci, 4:114-120.

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[6] Li, T., Danelisen, I., Singal, P.K., 2002. Early changes in myocardial antioxidant enzymes in rats treated with Adriamycin. Mol Cell Biochem, 232:19-26.

[7] Maeda, A., Honda, M., Kuramochi, T., Takabatake, T., 1998. Doxotabicin cardiotoxicity: Diastolic cardiac calcium handing in isolated cardiac myocytes. Jpn Circ, 62:505-511.

[8] Yang, G.M., Li, S.Q., Ye, S.Y., Li, J.L., Lin, S.X., 2002. The effects of fructose 1,6-diphosphate on tyrosine-nitro of cardiomyocytes in rats treated with Adriamycin. Chin Pharmcol Bull, 18:161-165.

[9] Zhou, Y., Wang, P., Lei, Z., He, H., Zhu, Z., 1999. Beneficial effects of fructose 1,6-diphosphate on hemorrahogic shock in rats. Chin J Traumatol, 15:22-24.

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