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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.7 P.637~643


Basic fibroblast growth factor alleviates brain injury following global ischemia reperfusion in rabbits

Author(s):  ZHANG Mao, MA Yue-feng, GAN Jian-xin, JIANG Guan-yu, XU Shan-xiang, TAO Xiang-luo, HONG An, LI Jiao-kun

Affiliation(s):  Department of Emergency Medicine, Second Affiliated Hospital, Zhejiang University, School of Medicine, Hangzhou 310009, China; more

Corresponding email(s):   zmhz@hotmail.com, yuefengma@hotmail.com

Key Words:  Basic fibroblast growth factor, Brain injury, Global ischemia, Neuron specific enolase, S-100B protein, Tumor necrosis factor-&alpha

ZHANG Mao, MA Yue-feng, GAN Jian-xin, JIANG Guan-yu, XU Shan-xiang, TAO Xiang-luo, HONG An, LI Jiao-kun. Basic fibroblast growth factor alleviates brain injury following global ischemia reperfusion in rabbits[J]. Journal of Zhejiang University Science B, 2005, 6(7): 637~643.

@article{title="Basic fibroblast growth factor alleviates brain injury following global ischemia reperfusion in rabbits",
author="ZHANG Mao, MA Yue-feng, GAN Jian-xin, JIANG Guan-yu, XU Shan-xiang, TAO Xiang-luo, HONG An, LI Jiao-kun",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Basic fibroblast growth factor alleviates brain injury following global ischemia reperfusion in rabbits
%A MA Yue-feng
%A GAN Jian-xin
%A JIANG Guan-yu
%A XU Shan-xiang
%A TAO Xiang-luo
%A LI Jiao-kun
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 7
%P 637~643
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0637

T1 - Basic fibroblast growth factor alleviates brain injury following global ischemia reperfusion in rabbits
A1 - ZHANG Mao
A1 - MA Yue-feng
A1 - GAN Jian-xin
A1 - JIANG Guan-yu
A1 - XU Shan-xiang
A1 - TAO Xiang-luo
A1 - HONG An
A1 - LI Jiao-kun
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 7
SP - 637
EP - 643
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0637

The aim of this study was to explore the protective effect of basic fibroblast growth factor (bFGF) on brain injury following global ischemia reperfusion and its mechanisms. brain injury following global ischemia was induced by four vessels occlusion and systemic hypotension. Twenty-four rabbits were randomized into three groups: group A, only dissection of vessels; group B, intravenous infusion of normal saline after reperfusion for 6 h; group C, 30 μg/kg bFGF injected intravenously at the onset of reperfusion, then infused with 10 μg/(kg·h) for 6 h. Serum neuron specific enolase (NSE), S-100B, tumor necrosis factor-&alpha; (TNF-α), interleukin-1 (IL-1), interleukin-8 (IL-8) were measured before ischemia, 30 min after ischemia, 0.5, 1, 3, 6 h after reperfusion. Brain water content was determined and cerebral histopathological damages were compared. NSE and S-100B were increased 1 h after reperfusion and reached their peaks 6 h after reperfusion, but were much higher in group B than those in group C 3, 6 h after reperfusion. In groups B and C, TNF-α was increased after ischemia and IL-1 and IL-8 were increased significantly 0.5 h after reperfusion, then reached their peaks 6 h, 3 h, 6 h after reperfusion respectively. TNF-α and IL-8 at the time points of 1 h and 3 h and IL-1 at 3 h and 6 h in group C were correspondingly lower than those in group B. These indices in group A were nearly unchanged. There were less severe cerebral histopathological damages in group C compared with group B, but no difference in brain water content. It could be concluded that bFGF alleviates brain injury following global ischemia and reperfusion by down-regulating expression of inflammatory factors and inhibiting their activities.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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2010-08-23 21:29:03

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