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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.8 P.759~769


Comparative study on glutathione transferases of rat brain and testis under the stress of phenobarbitol and β-methylcholanthrene


Affiliation(s):  Department of Biochemistry, Sri Venkateswara University, Tirupati 517 502, AP, India

Corresponding email(s):   Thyagarajuk_1999@yahoo.com

Key Words:  Glutathione transferases, Testis, Brain, Phenobarbitol, &beta, -Methylcholanthrene

THYAGARAJU K., HEMAVATHI B., VASUNDHARA K., RAO A.D., DEVI K.N.. Comparative study on glutathione transferases of rat brain and testis under the stress of phenobarbitol and β-methylcholanthrene[J]. Journal of Zhejiang University Science B, 2005, 6(8): 759~769.

@article{title="Comparative study on glutathione transferases of rat brain and testis under the stress of phenobarbitol and β-methylcholanthrene",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Comparative study on glutathione transferases of rat brain and testis under the stress of phenobarbitol and β-methylcholanthrene
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 8
%P 759~769
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0759

T1 - Comparative study on glutathione transferases of rat brain and testis under the stress of phenobarbitol and β-methylcholanthrene
A1 - RAO A.D.
A1 - DEVI K.N.
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 8
SP - 759
EP - 769
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0759

A comparative study was made on the tissue specific expression of glutathione transferases (GST) in brain and testis after exposure of rat to phenobarbitol (PB) and b-Methylcholanthrene (MC). glutathione transferases, a family of multifunctional proteins are involved in intracellular transport processes and in detoxication of electrophilic xenobiotics by catalyzing reactions such as conjugation, isomerization, reduction and thiolysis. On purification, the yield of GST proteins by affinity chromatography was 39% in testis and 32% in brain. The affinity purified testis GSTs were resolved by chromatofocusing into six anionic and four cationic isozymes, and in brain glutathione transferases were resolved into four anionic and three cationic isozymes, suggesting the presence of multiple isozymes with Yc, Yb, Y&beta; and Yδ in both of them. In testis and brain, these isozymes at identical pI values showed variable functions with a battery of substrates and the cationic isozymes of brain and testis showed identical properties in CHP (cumene hydroperoxide) at pH values of above 7.0. Substrate specificity studies and immunoblot analysis of testis and brain proteins revealed that they play a predominant role in the detoxication of phenobarbitol or &beta;-Methylcholanthrene. Expression of the isozymes in testis and brain on exposure to PB and MC indicated elevated subunit variation. In both testis and brain, Yδ of π class was expressed on PB treatment and Yc of α class and Y&beta; of μ class was expressed in MC treated testis and only Yc was predominantly expressed in MC treated brain. Thus these subunits expression is considered as markers for carcinogenesis and specific to chemical toxicity under phenobarbitol and &beta;-Methylcholanthrene stress.

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