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Received: 2005-03-10

Revision Accepted: 2005-04-30

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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.8 P.770~777

http://doi.org/10.1631/jzus.2005.B0770


Survival of the biocontrol agents Brevibacillus brevis ZJY-1 and Bacillus subtilis ZJY-116 on the spikes of barley in the field


Author(s):  ZHANG Xin, ZHANG Bing-xin, ZHANG Zhen, SHEN Wei-feng, YANG Ching-hong, YU Jing-quan, ZHAO Yu-hua

Affiliation(s):  Department of Plant Protection, School of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   bxzhang@zju.edu.cn

Key Words:  GFP, Survival, Brevibacillus brevis and Bacillus subtilis, Spikes, Barley, Biocontrol


ZHANG Xin, ZHANG Bing-xin, ZHANG Zhen, SHEN Wei-feng, YANG Ching-hong, YU Jing-quan, ZHAO Yu-hua. Survival of the biocontrol agents Brevibacillus brevis ZJY-1 and Bacillus subtilis ZJY-116 on the spikes of barley in the field[J]. Journal of Zhejiang University Science B, 2005, 6(8): 770~777.

@article{title="Survival of the biocontrol agents Brevibacillus brevis ZJY-1 and Bacillus subtilis ZJY-116 on the spikes of barley in the field",
author="ZHANG Xin, ZHANG Bing-xin, ZHANG Zhen, SHEN Wei-feng, YANG Ching-hong, YU Jing-quan, ZHAO Yu-hua",
journal="Journal of Zhejiang University Science B",
volume="6",
number="8",
pages="770~777",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B0770"
}

%0 Journal Article
%T Survival of the biocontrol agents Brevibacillus brevis ZJY-1 and Bacillus subtilis ZJY-116 on the spikes of barley in the field
%A ZHANG Xin
%A ZHANG Bing-xin
%A ZHANG Zhen
%A SHEN Wei-feng
%A YANG Ching-hong
%A YU Jing-quan
%A ZHAO Yu-hua
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 8
%P 770~777
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0770

TY - JOUR
T1 - Survival of the biocontrol agents Brevibacillus brevis ZJY-1 and Bacillus subtilis ZJY-116 on the spikes of barley in the field
A1 - ZHANG Xin
A1 - ZHANG Bing-xin
A1 - ZHANG Zhen
A1 - SHEN Wei-feng
A1 - YANG Ching-hong
A1 - YU Jing-quan
A1 - ZHAO Yu-hua
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 8
SP - 770
EP - 777
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0770


Abstract: 
Fusarium head blight (FHB) caused by Fusarium graminearum is a devastating disease that results in extensive yield losses to wheat and barley. A green fluorescent protein (GFP) expressing plasmid pRP22-GFP was constructed for monitoring the colonization of two biocontrol agents, Brevibacillus brevis ZJY-1 and Bacillus subtilis ZJY-116, on the spikes of barley and their effect on suppression of FHB. survival and colonization of the Brevibacillus brevis ZJY-1 and Bacillus subtilis ZJY-116 strains on spikes of barley were observed by tracking the bacterial transformants with GFP expression. Our field study revealed that plasmid pRP22-GFP was stably maintained in the bacterial strains without selective pressure. The retrieved GFP-tagged strains showed that the bacterial population fluctuation accorded with that of the rain events. Furthermore, both biocontrol strains gave significant protection against FHB on spikes of barley in fields. The greater suppression of barley FHB disease was resulted from the treatment of barley spikes with biocontrol agents before inoculation with F. graminearum.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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