Full Text:   <1344>

CLC number: Q945

On-line Access: 

Received: 2005-01-20

Revision Accepted: 2005-04-29

Crosschecked: 0000-00-00

Cited: 10

Clicked: 4067

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.8 P.803~811

http://doi.org/10.1631/jzus.2005.B0803


Isolation and physiological characteristics of a premature senescence mutant in rice (Oryza sativa L.)


Author(s):  LI Fu-zhen, JIN Song-heng, HU Guo-cheng, FU Ya-ping, SI Hua-min, JIANG De-an, SUN Zong-xiu

Affiliation(s):  State Key Laboratory of Plant Physiology and Biochemistry, School of Life Sciences, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   dajiang@zju.edu.cn, sunzx@hzcnc.com

Key Words:  Antioxidative enzymes, Net photosynthesis rate, Peptidase, Phytotron, Premature senescence mutant, Rice (Oryza sativa L.)


LI Fu-zhen, JIN Song-heng, HU Guo-cheng, FU Ya-ping, SI Hua-min, JIANG De-an, SUN Zong-xiu. Isolation and physiological characteristics of a premature senescence mutant in rice (Oryza sativa L.)[J]. Journal of Zhejiang University Science B, 2005, 6(8): 803~811.

@article{title="Isolation and physiological characteristics of a premature senescence mutant in rice (Oryza sativa L.)",
author="LI Fu-zhen, JIN Song-heng, HU Guo-cheng, FU Ya-ping, SI Hua-min, JIANG De-an, SUN Zong-xiu",
journal="Journal of Zhejiang University Science B",
volume="6",
number="8",
pages="803~811",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B0803"
}

%0 Journal Article
%T Isolation and physiological characteristics of a premature senescence mutant in rice (Oryza sativa L.)
%A LI Fu-zhen
%A JIN Song-heng
%A HU Guo-cheng
%A FU Ya-ping
%A SI Hua-min
%A JIANG De-an
%A SUN Zong-xiu
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 8
%P 803~811
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0803

TY - JOUR
T1 - Isolation and physiological characteristics of a premature senescence mutant in rice (Oryza sativa L.)
A1 - LI Fu-zhen
A1 - JIN Song-heng
A1 - HU Guo-cheng
A1 - FU Ya-ping
A1 - SI Hua-min
A1 - JIANG De-an
A1 - SUN Zong-xiu
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 8
SP - 803
EP - 811
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0803


Abstract: 
A rice pse(t) (premature senescence, tentatively) mutant line, was isolated from 4 500 independent T-DNA inserted transgenic lines. The symptoms of premature senescence appeared more severely than those of the control plants (Zhonghua 11, japonica) at the last development stage. To characterize the mutant and provide basic information on the candidate genes by mapping to a physical region of 220-kb, experiments were carried out in two phytotrons under controlled temperature of 24 °C and 28 °C, respectively. The content of chlorophyll, soluble protein and MDA (malondialdehyde), net photosynthesis, the antioxidant enzyme activities of SOD (superoxide dismuase) (EC 1.15.1.1) and POD (peroxidase) (EC 1.11.1.7) and the peptidase activities of leaves were measured from top to bottom according to the leaf positions at the flowering stage. Compared with the control plant, the mutant showed the following characteristics: (1) Higher net photosynthesis rate (Pn) appeared in the 1st and 2nd leaves, contents of chlorophyll and soluble protein were also higher in the 1st leaf; (2) The activities of SOD, POD and peptidase were higher according to the leaf position from top to bottom; (3) The symptom of premature senescence was accelerated in the mutant at 28 °C treatment. The MDA content and the SOD and POD activities between the 24 °C and 28 °C treatment mutants were not significantly different. Content of chlorophyll and soluble protein of leaves mutant decreased rapidly at 28 °C treatment. The results show that pse(t) is sensitive to high temperature. The probable function of PSE(T) is discussed.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1] Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 7(2):248-254.

[2] Chen, Y., 1999. The Determination of Protease (Peptidase). Modern Plant Physiology: A Laboratory Manual. Science Press, Beijing, China, p.149-151 (in Chinese).

[3] Del Rio, L.A., Pastori, G.M., Palma, J.M., Sandalio, L.M., Sevilla, F., Corpas, F.J., Jimenez, A., Lopez-Huertas, E., Hernandez, J.A., 1998. The activated oxygen role of peroxisomes in senescence. Plant Physiol., 116(4):1195-1200.

[4] Duan, J., Liang, C.Y., Huang, Y.W., 1997. Studies on leaf senescence of hybrid rice at flowering and grain formation stage. Acta Phytophysiologica Sinaca, 23(2):139-144 (in Chinese with English abstract).

[5] Foyer, C.H., Vanacker, H., Gomez, L.D., Harbinson, J., 2002. Regulation of photosynthesis and antioxidant metabolism in maize leaves at optimal and chilling temperatures: Review. Plant Physiol. Biochem., 40(5):659-668.

[6] Giannopolitis, C.N., Ries, S.K., 1977. Superoxide dismutase in higher plants. Plant Physiol., 59:309-314.

[7] Hill, C.M., Pearson, S.A., Smith, A.J., Rogers, L.J., 1985. Inhibition of chlorophyll synthesis in Hordeum vulgare by 3-amino-2,3-dihydrobenzoic acid (gabaculin). Bioscience Report, 5(9):775-781.

[8] Izumi, O., Kuniyuki, S, Toshirou, K., 2004. Effects of Rising Temperature on Growth, Yield and Dry-matter Production of Rice Grown in the Paddy Field. The 4th International Crop Science Congress, Brisbane, Australia, p.1-4.

[9] Jiang, C.Z., Rodermel, S.R., 1995. Regulation of photosynthesis during leaf development in RbcS antisense DNA mutants of tobacco. Plant Physiol., 107(1):215-224.

[10] Jing, H.C., Sturre, M.J., Hille, J., Dijkwel, P.P., 2002. Arabidopsis onset of leaf death mutants identify a regulatory pathway controlling leaf senescence. Plant J., 32(1):51-63.

[11] Lal, A.M., Ku, S.B., Edwards, G.E., 1996. Analysis of inhibition of photosynthesis due to water stress in the C3 species Hordeum vulgare and Vicia faba: Electron transport, CO2 fixation and carboxylation capacity. Photosynth Res., 49(1):57-69.

[12] Lewis, J.D., Wang, X.Z., Griffin, K.L., Tissue, D.T., 2002. Effects of age and ontogeny on photosynthetic responses of a determinate annual plant to elevated CO2 concentrations. Plant Cell Environ., 25(3):359-368.

[13] Li, X., Jiao, D.M., Liu, Y.L., Huang, X.Q., 2002. Chlorophyll fluorescence membrance lipid peroxidation in the flag leaves of different high yield rice variety at late stage of development under nature condition. Acta Botanica Sinaca, 44(4):413-421.

[14] Li, F.Z., Hu, G.C., Fu, Y.P, Si, H.M., Bai, X.M., Sun, Z.X., 2005. Genetic analysis and high-resolution mapping a premature senescence gene ‘Pse(t)’ in rice. Genome (in press).

[15] Lim, P.O., Woo, H.R., Nam, H.G., 2003. Molecular genetics of leaf senescence in Arabidopsis. Trends Plant Sci., 8(6):272-278.

[16] Lu, D.Z., Pan, Y.C., Ma, Y.F., Lin, Z.D., Bao, W.Q., Jin, Y.M., You, S.F., 1988. Physiology and biochemical studies on leaf senescence at heading and grain formation stage in hybrid rice. Science Agriculture Sinaca, 21(1):21-26 (in Chinese with English abstract).

[17] Madhava, R.K.V., Sresty, T.V.S., 2000. Antioxidative parameters in the seedlings of pigeonpea (Cajanus cajan L. Millspaugh) in response to Zn and Ni stresses. Plant Sci., 157(1):113-128.

[18] Matile, P., Hortensteiner, S., Thomas, H., 1999. Chlorophyll degradation. Annu. Rev. Plant Physiol. Plant Mol. Biol., 50(1):67-95.

[19] Misr, A.N., Meena, M., 1986. Effect of temperature on senescing detached rice leaves: I. Photoelectron transport activity of chloroplasts. Plant Sci., 46(1):1-4.

[20] Nam, H.G., 1997. The molecular genetic analysis of leaf senescence. Curr. Opin. Biotechnol., 8(2):200-207.

[21] Normile, D., 1999. Crossing rice strains to keep Asia’s rice bowls brimming. Science, 283(5400):313.

[22] Pourtau, N., Mares, M., Purdy, S., Quentin, N., Ruel, A., Wingler, A., 2004. Interactions of abscisic acid and sugar signalling in the regulation of leaf senescence. Planta, 219(5):765-772.

[23] Pruzinska, A., Tanner, G., Anders, I., Roca, M., Hortensteiner, S., 2003. Chlorophyll breakdown: Pheophorbide a oxygenase is a rieske-type iron-sulfur protein, encoded by the accelerated cell death 1 gene. Proc. Natl. Acad. Sci., 100(25):15259-15264.

[24] Quick, W.P., Chaves, M.M., Wendler, R., David, M., Rodrigues, M.L., Passaharinho, J.A., Pereira, J.S., 1992. The effect of water stress on photosynthetic carbon metabolism in four species grown under field conditions. Plant Cell Environ., 15(1):25-35.

[25] Sun, Z.X., Fu, Y.P., Zhu, Z.G., Xiao, H., Zhang, J.L., Zhang, H.X., Hu, G.C., Yu, Y.H., Si, H.M., Hong, M.M., 2003. Construction of Three Transgenic Rice Populations by Maize Transposable Element Ac/Ds Mutagenesis via Agrobacterium Tumefaciens. In: Khush, G.S., Brar, D.S., Hardy, B. (Eds.), Advances in Rice Genetics. IRRI, DAPO Box 7777, Metro Manila, Philippines, p.547-549.

[26] Takahashi, A., Kawasaki, T., Wong, H.L., Suharsono, U., Hirano, H., Shimamoto, K., 2003. Hyperphosphorylation of a mitochondrial protein, prohibitin, is induced by calyculin A in a rice lesion-mimic mutant cdr1. Plant Physiol., 132(4):1861-1869.

[27] Wu, F., Zhang, G., Dominy, P., 2003. Four barley genotypes respond differently to cadmium: Lipid peroxidation and activities of antioxidant capacity. Environ. Exp. Bot., 50(1):67-78.

[28] Xu, L.L., Ye, M.B., 1989. A measurement of peroxidase activity using continuous recording method. J. Nanjing Agricult. Univ., 12(3):82-83 (in Chinese).

[29] Yang, J.C., Peng, S.B., Zhang, Z.J., Wang, Z.Q., Visperas, R.M., Zhu, Q.S., 2002. Grain and dry matter yields and partitioning of assimilates in japonica/indica hybrid rice. Crop Sci., 42(3):766-772.

[30] Yang, M., Wardzala, E., Johal, G.S., Gray, J., 2004. The wound-inducible Lls1 gene from maize is an orthologue of the Arabidopsis Acd1 gene, and the LLS1 protein is present in non-photosynthetic tissues. Plant Mol. Biol., 54(2):175-191.

[31] Zhu, Z.G., Xiao, H., Fu, Y.P., Hu, G.C., Yu, Y.H., Si, H.M., Zhang, J.L., Sun, Z.X., 2001. Construction of transgenic rice populations by inserting the maize transponson Ac/Ds and genetic analysis for several mutants. Chin. J. Biotech., 17(3):288-292 (in Chinese with English abstract).

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE