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CLC number: R392.11
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Received: 2004-12-29
Revision Accepted: 2005-04-09
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Immunomodulatory function of orally administered thymosin α1
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CHEN Xiang-ming, JIANG Han-liang, ZHOU Lin-fu, PAN Xiao-ping, HU Zhong-rong, LIU Rong-hua, CHEN Xiao-ming, CHEN Zhi. Immunomodulatory function of orally administered thymosin α1[J]. Journal of Zhejiang University Science B, 2005, 6(9): 873-876.
@article{title="Immunomodulatory function of orally administered thymosin α1",
author="CHEN Xiang-ming, JIANG Han-liang, ZHOU Lin-fu, PAN Xiao-ping, HU Zhong-rong, LIU Rong-hua, CHEN Xiao-ming, CHEN Zhi",
journal="Journal of Zhejiang University Science B",
volume="6",
number="9",
pages="873-876",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B0873"
}
%0 Journal Article
%T Immunomodulatory function of orally administered thymosin α1
%A CHEN Xiang-ming
%A JIANG Han-liang
%A ZHOU Lin-fu
%A PAN Xiao-ping
%A HU Zhong-rong
%A LIU Rong-hua
%A CHEN Xiao-ming
%A CHEN Zhi
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 9
%P 873-876
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0873
TY - JOUR
T1 - Immunomodulatory function of orally administered thymosin α1
A1 - CHEN Xiang-ming
A1 - JIANG Han-liang
A1 - ZHOU Lin-fu
A1 - PAN Xiao-ping
A1 - HU Zhong-rong
A1 - LIU Rong-hua
A1 - CHEN Xiao-ming
A1 - CHEN Zhi
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 9
SP - 873
EP - 876
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0873
Abstract: Objective: To investigate the immunological function of a yeast expression system for thymosin α1 (Tα1). Methods: A constructed Tα1 yeast expression system was used to investigate the immunological function of orally administered Tα1. Dried yeast containing three different concentration of Tα1 was fed to normal Balb/c mice and other Balb/c mice whose immunities were inhibited in advance by cyclophosphamide. Synthesized Tα1 peptide was used as positive control and dried yeast with empty plasmid was used as negative control. CD4+ and CD8+ levels were detected by flow cytometry assay. TNF-α, IFN-γ, IL-2, IL-6 and IL-10 levels were detected by liquid chip. Results: In normal Balb/c mice or immune inhibition Balb/c mice, CD8+ levels were significantly increased. Especially in immune inhibition Balb/c mice, CD8+ levels in synthesized Tα1 group (18.77%±4.72%), small dose group (13.48%±6.17%) and large dose group (22.74%±1.09%) were significantly higher than that in empty yeast control group (7.49%±2.14%). Conclusion: orally administered Tα1 has its certain immunomodulatory function.
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