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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.6 P.421~428

10.1631/jzus.2006.B0421


Proliferation of endothelial cell on polytetrafluoroethylene vascular graft materials carried VEGF gene plasmid


Author(s):  TAO Si-feng, CHEN Li, ZHENG Yi-xiong, XU Yuan, CHEN Jian, YU Hong

Affiliation(s):  Department of Surgery, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China; more

Corresponding email(s):   chenli@mail.hz.zj.cn

Key Words:  Polytetrafluoroethylene, Vascular endothelial growth factor, Vascular grafts, Gene, Endothelial cell


TAO Si-feng, CHEN Li, ZHENG Yi-xiong, XU Yuan, CHEN Jian, YU Hong. Proliferation of endothelial cell on polytetrafluoroethylene vascular graft materials carried VEGF gene plasmid[J]. Journal of Zhejiang University Science B, 2006, 7(6): 421~428.

@article{title="Proliferation of endothelial cell on polytetrafluoroethylene vascular graft materials carried VEGF gene plasmid",
author="TAO Si-feng, CHEN Li, ZHENG Yi-xiong, XU Yuan, CHEN Jian, YU Hong",
journal="Journal of Zhejiang University Science B",
volume="7",
number="6",
pages="421~428",
year="2006",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2006.B0421"
}

%0 Journal Article
%T Proliferation of endothelial cell on polytetrafluoroethylene vascular graft materials carried VEGF gene plasmid
%A TAO Si-feng
%A CHEN Li
%A ZHENG Yi-xiong
%A XU Yuan
%A CHEN Jian
%A YU Hong
%J Journal of Zhejiang University SCIENCE B
%V 7
%N 6
%P 421~428
%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0421

TY - JOUR
T1 - Proliferation of endothelial cell on polytetrafluoroethylene vascular graft materials carried VEGF gene plasmid
A1 - TAO Si-feng
A1 - CHEN Li
A1 - ZHENG Yi-xiong
A1 - XU Yuan
A1 - CHEN Jian
A1 - YU Hong
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 6
SP - 421
EP - 428
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2006.B0421


Abstract: 
Objective: To investigate whether vascular endothelial growth factor (VEGF) gene plasmid carried by polytetrafluoroethylene (PTFE) vascular graft materials could transfect endothelial cells (ECs) and promote their growth. Methods: PTFE vascular graft materials carried with pCDI-hVEGF121, pCDI or pEGFP were incubated in Tris-buffer solution and the values of optical density of 260 nm at different time were plotted, then the DNA controlled release curve was made. ECs derived from human umbilical vein were seeded on the pCDI-hVEGF121/pCDI/pEGFP-PTFE materials or tissue culture plates, ECs numbers were counted and VEGF protein concentrations at different time were measured by enzyme-linked immunoadsorbent assay method. Green fluorescent protein (GFP) expression in ECs on pEGFP-PTFE materials was examined with fluorescence microscopy. Results: The controlled release curve showed that the gene released from PTFE materials was rapid within 8 h, then slowed down and that the gene released continuously even after 72 h. At 24, 72 and 120 h, ECs number and proliferation rate of pCDI-hVEGF121-PTFE materials were higher than those of pCDI or pEGFP-PTFE materials (P<0.05). VEGF protein concentration of pCDI-hVEGF121-PTFE materials was higher than that of pCDI or pEGFP-PTFE materials at 6, 24, 72 and 120 h (P<0.01). GFP expression in ECs on the pEGFP-PTFE materials could be detected by fluorescence microscopy. Conclusion: PTFE graft can be used as a carrier of VEGF gene plasmid, VEGF gene carried by PTFE can transfect ECs and promote ECs growth.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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