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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.6 P.482~490


Optimization of elastolysis conditions and elastolytic kinetic analysis with elastase from Bacillus licheniformis ZJUEL31410

Author(s):  CHEN Qi-he, HE Guo-qing

Affiliation(s):  Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310029, China

Corresponding email(s):   qhchenzju@yahoo.com.cn, gqhe@zju.edu.cn

Key Words:  Bacillus licheniformis, Elastase, Elastolysis condition optimization, Elastolytic kinetics

CHEN Qi-he, HE Guo-qing. Optimization of elastolysis conditions and elastolytic kinetic analysis with elastase from Bacillus licheniformis ZJUEL31410[J]. Journal of Zhejiang University Science B, 2006, 7(6): 482~490.

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%A HE Guo-qing
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T1 - Optimization of elastolysis conditions and elastolytic kinetic analysis with elastase from Bacillus licheniformis ZJUEL31410
A1 - CHEN Qi-he
A1 - HE Guo-qing
J0 - Journal of Zhejiang University Science B
VL - 7
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SP - 482
EP - 490
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2006.B0482

The solubilization of elastin by Bacillus licheniformis elastase cannot be analyzed by conventional kinetic methods because the biologically relevant substrate is insoluble and the concentration of enzyme-substrate complex has no physical meaning. In this paper we report the optimization of elastolysis conditions and analysis of elastolytic kinetics. Our results indicated that the hydrolyzing temperature and time are very important factors affecting elastolysis rate. The optimized conditions using central composite design were as follows: elastolysis temperature 50 °C, elastase concentration 1×104 U/ml, elastin 80 mg, elastolytic time 4 h. Investigation of the effects of substrate content, elastase concentration and pH was also revealed that low or high elastin content inhibits the elastolysis process. Increasing elastase improves elastin degradation, but high elastase may change the kinetics characterization. Alkaline environment can decrease elastin degradation rate and pH may affect elastolysis by changing elastase reaction pH. To further elucidate the elastolysis process, the logistic model was used to elastolysis kinetics study showing clearly that the logistic model can reasonably explain the elastolysis process, especially under lower elastase concentration. However, there is still need for more investigations with the aid of other methods, such as biochemical and molecular methods.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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