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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.8 P.641~647

http://doi.org/10.1631/jzus.2006.B0641


Bone marrow mesenchymal stem cell transplantation combined with perindopril treatment attenuates infarction remodelling in a rat model of acute myocardial infarction


Author(s):  WANG Jian-an, LUO Rong-hua, ZHANG Xing, XIE Xiao-jie, HU Xin-yang, HE Ai-na, CHEN Jie, LI Jia-hui

Affiliation(s):  Department of Cardiology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China; more

Corresponding email(s):   wang_jian_an@tom.com

Key Words:  Remodelling, Acute myocardial infarction, Perindopril, Bone marrow mesenchymal stem cell


WANG Jian-an, LUO Rong-hua, ZHANG Xing, XIE Xiao-jie, HU Xin-yang, HE Ai-na, CHEN Jie, LI Jia-hui. Bone marrow mesenchymal stem cell transplantation combined with perindopril treatment attenuates infarction remodelling in a rat model of acute myocardial infarction[J]. Journal of Zhejiang University Science B, 2006, 7(8): 641~647.

@article{title="Bone marrow mesenchymal stem cell transplantation combined with perindopril treatment attenuates infarction remodelling in a rat model of acute myocardial infarction",
author="WANG Jian-an, LUO Rong-hua, ZHANG Xing, XIE Xiao-jie, HU Xin-yang, HE Ai-na, CHEN Jie, LI Jia-hui",
journal="Journal of Zhejiang University Science B",
volume="7",
number="8",
pages="641~647",
year="2006",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2006.B0641"
}

%0 Journal Article
%T Bone marrow mesenchymal stem cell transplantation combined with perindopril treatment attenuates infarction remodelling in a rat model of acute myocardial infarction
%A WANG Jian-an
%A LUO Rong-hua
%A ZHANG Xing
%A XIE Xiao-jie
%A HU Xin-yang
%A HE Ai-na
%A CHEN Jie
%A LI Jia-hui
%J Journal of Zhejiang University SCIENCE B
%V 7
%N 8
%P 641~647
%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0641

TY - JOUR
T1 - Bone marrow mesenchymal stem cell transplantation combined with perindopril treatment attenuates infarction remodelling in a rat model of acute myocardial infarction
A1 - WANG Jian-an
A1 - LUO Rong-hua
A1 - ZHANG Xing
A1 - XIE Xiao-jie
A1 - HU Xin-yang
A1 - HE Ai-na
A1 - CHEN Jie
A1 - LI Jia-hui
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 8
SP - 641
EP - 647
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2006.B0641


Abstract: 
Objective: This study was performed to evaluate whether implantation of mesenchymal stem cell (MSC) would reduce left ventricular remodelling from the molecular mechanisms compared with angiotensin-converting enzyme inhibitors (ACEIs) perindopril into ischemic myocardium after acute myocardial infarction. Methods: Forty rats were divided into four groups: control, MSC, ACEI, MSC+ACEI groups. Bone marrow stem cell derived rat was injected immediately into a zone made ischemic by coronary artery ligation in MSC group and MSC+ACEI group. Phosphate-buffered saline (PBS) was injected into control group. perindopril was administered p.o. to ACEI group and MSC+ACEI group. Six weeks after implantation, the rats were killed and heart sample was collected. Fibrillar collagen was observed by meliorative Masson’s trichome stain. Western Blotting was employed to evaluate the protein expression of matrix metalloproteinase (MMP)-2, matrix metalloproteinase (MMP)-9 in infarction zone. The transcriptional level of MMP2, MMP9 and tissue inhibitor of matrix metalloproteinase (TIMP)-1 in infarction area was detected by reverse transcriptase PCR (RT-PCR) analysis. Results: The fibrillar collagen area, the protein expression of MMP2, MMP9 and the transcriptional level of MMP2, MMP9 mRNA in infarction zone reduced in MSC group, ACEI group, and MSC+ACEI group. No significant difference was detected in the expression of TIMP1 mRNA among the 4 groups. Conclusion: Both MSC and ACEI could reduce infarction remodelling by altering collagen metabolism.

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Reference

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