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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.9 P.690~695

10.1631/jzus.2006.B0690


Effect of selected fungi on the reduction of gossypol levels and nutritional value during solid substrate fermentation of cottonseed meal


Author(s):  ZHANG Wen-ju, XU Zi-rong, SUN Jian-yi, YANG Xia

Affiliation(s):  Key Laboratory of Molecular Animal Nutrition of Ministry of Education, College of Animal Science, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   zhang-wj1022@tom.com, zrxu@zju.edu.cn

Key Words:  Fungi, Free gossypol, Solid substrate fermentation, Cottonseed meal, Detoxification, Nutritional value


ZHANG Wen-ju, XU Zi-rong, SUN Jian-yi, YANG Xia. Effect of selected fungi on the reduction of gossypol levels and nutritional value during solid substrate fermentation of cottonseed meal[J]. Journal of Zhejiang University Science B, 2006, 7(9): 690~695.

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author="ZHANG Wen-ju, XU Zi-rong, SUN Jian-yi, YANG Xia",
journal="Journal of Zhejiang University Science B",
volume="7",
number="9",
pages="690~695",
year="2006",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2006.B0690"
}

%0 Journal Article
%T Effect of selected fungi on the reduction of gossypol levels and nutritional value during solid substrate fermentation of cottonseed meal
%A ZHANG Wen-ju
%A XU Zi-rong
%A SUN Jian-yi
%A YANG Xia
%J Journal of Zhejiang University SCIENCE B
%V 7
%N 9
%P 690~695
%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0690

TY - JOUR
T1 - Effect of selected fungi on the reduction of gossypol levels and nutritional value during solid substrate fermentation of cottonseed meal
A1 - ZHANG Wen-ju
A1 - XU Zi-rong
A1 - SUN Jian-yi
A1 - YANG Xia
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 9
SP - 690
EP - 695
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2006.B0690


Abstract: 
The objective of this work was to investigate the effect of six individual strains of fungi on the reduction of gossypol levels and nutritional value during solid substrate fermentation of cottonseed meal (CSM). Six groups of disinfected CSM substrate were incubated for 48 h after inoculation with either of the fungi C. capsuligena ZD-1, C. tropicalis ZD-3, S. cerevisae ZD-5, A. terricola ZD-6, A. oryzae ZD-7, or A. niger ZD-8. One not inoculated group (substrate) was used as a control. Levels of initial and final free gossypol (FG), crude protein (CP), amino acids (AA) and in vitro digestibility were assayed. The experiment was done in triplicate. The experimental results indicated that microbial fermentation could greatly decrease (P<0.05) FG levels in CSM. The detoxification efficiency differed between the species of microorganisms applied. From the perspective of reducing CSM potential toxicity, C. tropicalis ZD-3 was most successful followed by S. cerevisae ZD-5 and A. niger ZD-8. They could reduce FG levels of CSM to 29.8, 63.07 and 81.50 mg/kg based on DM (dry matter), respectively, and their detoxification rates were 94.57%, 88.51% and 85.16%, respectively. If crude protein, amino acids content and their in vitro digestibility were also taken into account, A. niger ZD-8 may be the best choice. The CP content of CSM substrate fermented by C. tropicalis ZD-3 and A. niger ZD-8 were improved by 10.76% and 22.24%; the TAA (total amino acids) contents were increased by 7.06% and 11.46%, and the EAA (essential amino acids) were raised by 7.77% and 12.64%, respectively. Especially, the levels of methionine, lysine and threonine were improved greatly (P<0.05). The in vitro CP digestibility of CSM fermented by C. tropicalis ZD-3 and A. niger ZD-8 was improved by 13.42% and 18.22%, the TAA were increased by 17.75% and 22.88%, and the EAA by 16.61% and 21.01%, respectively. In addition, the in vitro digestibility of methionine, lysine and threonine was also improved greatly (P<0.05).

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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