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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.10 P.830~836

http://doi.org/10.1631/jzus.2006.B0830


Inactivation of the tumor suppressor Krüppel-like factor 6 (KLF6) by mutation or decreased expression in hepatocellular carcinomas


Author(s):  PAN Xiu-cheng, CHEN Zhi, CHEN Feng, CHEN Xiao-hong, JIN Han-yin, XU Xiao-yan

Affiliation(s):  Institute of Infectious Disease, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China

Corresponding email(s):   chenzhi@zju.edu.cn

Key Words:  Tumor suppressor gene, Krü, ppel-like factor 6 (KLF6), Mutation, Gene expression, Hepatocellular carcinoma


PAN Xiu-cheng, CHEN Zhi, CHEN Feng, CHEN Xiao-hong, JIN Han-yin, XU Xiao-yan. Inactivation of the tumor suppressor Krüppel-like factor 6 (KLF6) by mutation or decreased expression in hepatocellular carcinomas[J]. Journal of Zhejiang University Science B, 2006, 7(10): 830~836.

@article{title="Inactivation of the tumor suppressor Krüppel-like factor 6 (KLF6) by mutation or decreased expression in hepatocellular carcinomas",
author="PAN Xiu-cheng, CHEN Zhi, CHEN Feng, CHEN Xiao-hong, JIN Han-yin, XU Xiao-yan",
journal="Journal of Zhejiang University Science B",
volume="7",
number="10",
pages="830~836",
year="2006",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2006.B0830"
}

%0 Journal Article
%T Inactivation of the tumor suppressor Krüppel-like factor 6 (KLF6) by mutation or decreased expression in hepatocellular carcinomas
%A PAN Xiu-cheng
%A CHEN Zhi
%A CHEN Feng
%A CHEN Xiao-hong
%A JIN Han-yin
%A XU Xiao-yan
%J Journal of Zhejiang University SCIENCE B
%V 7
%N 10
%P 830~836
%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0830

TY - JOUR
T1 - Inactivation of the tumor suppressor Krüppel-like factor 6 (KLF6) by mutation or decreased expression in hepatocellular carcinomas
A1 - PAN Xiu-cheng
A1 - CHEN Zhi
A1 - CHEN Feng
A1 - CHEN Xiao-hong
A1 - JIN Han-yin
A1 - XU Xiao-yan
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 10
SP - 830
EP - 836
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2006.B0830


Abstract: 
Background and aim: The krü;ppel-like transcription factor KLF6 is a novel tumor-suppressor gene. It was inactivated in human prostate cancer and other tumors tissue, as the result of frequent mutation and loss of heterozygosity (LOH). However, there is no data reporting the levels of KLF6 both mRNA and protein in hepatocellular carcinomas (HCCs). We therefore detected mutations and expression of KLF6 in HCC tissues and further observed the effect of it on cell growth in HCC cell lines. Methods: We analyzed the exon-2 of KLF6 gene by direct DNA sequencing, and detected the expression of KLF6 by RT-PCR and Western blot in 23 HCC tissues and corresponding nontumorous tissues. Loss of growth suppressive effect of the HCC-derived KLF6 mutant was characterized by in vitro growth curves plotted, flow cytometry and Western blotting. Results: KLF6 mutations were found in 2 of 23 HCC tissues and one of mutations was missense. Expression of KLF6 mRNA or protein was down-regulated in 8 (34.7%) or 9 (39.1%) of 23 HCC tissues. Wild-type KLF6 (wtKLF6) inhibited cellular proliferation and prolonged G1-S transition by inducing the expression of p21WAF1 following stable transfection into cultured HepG2 cells, but tumor-derived KLF6 mutant (mKLF6) had no effects. Conclusion: Our findings suggest that KLF6 may be involved in pathogenesis of HCC.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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