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Journal of Zhejiang University SCIENCE B 2007 Vol.8 No.2 P.88~97

10.1631/jzus.2007.B0088


Application of cDNA array for studying the gene expression profile of mature appressoria of Magnaporthe grisea


Author(s):  JIN Qing-chao, DONG Hai-tao, PENG You-liang, CHEN Bao-shan, SHAO Jing, DENG Ye, DAI Cheng-en, FANG Yong-qi, LOU Yi-chun, LI You-zhi, LI De-bao

Affiliation(s):  Bioinformatics and Gene Network Research Group, School of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   htdong@zjuem.zju.edu.cn, Lidb@mail.hz.zj.cn

Key Words:  Magnaporthe grisea, Mature appressoria, cDNA array, Gene expression profile


JIN Qing-chao, DONG Hai-tao, PENG You-liang, CHEN Bao-shan, SHAO Jing, DENG Ye, DAI Cheng-en, FANG Yong-qi, LOU Yi-chun, LI You-zhi, LI De-bao. Application of cDNA array for studying the gene expression profile of mature appressoria of Magnaporthe grisea[J]. Journal of Zhejiang University Science B, 2007, 8(2): 88~97.

@article{title="Application of cDNA array for studying the gene expression profile of mature appressoria of Magnaporthe grisea",
author="JIN Qing-chao, DONG Hai-tao, PENG You-liang, CHEN Bao-shan, SHAO Jing, DENG Ye, DAI Cheng-en, FANG Yong-qi, LOU Yi-chun, LI You-zhi, LI De-bao",
journal="Journal of Zhejiang University Science B",
volume="8",
number="2",
pages="88~97",
year="2007",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2007.B0088"
}

%0 Journal Article
%T Application of cDNA array for studying the gene expression profile of mature appressoria of Magnaporthe grisea
%A JIN Qing-chao
%A DONG Hai-tao
%A PENG You-liang
%A CHEN Bao-shan
%A SHAO Jing
%A DENG Ye
%A DAI Cheng-en
%A FANG Yong-qi
%A LOU Yi-chun
%A LI You-zhi
%A LI De-bao
%J Journal of Zhejiang University SCIENCE B
%V 8
%N 2
%P 88~97
%@ 1673-1581
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.B0088

TY - JOUR
T1 - Application of cDNA array for studying the gene expression profile of mature appressoria of Magnaporthe grisea
A1 - JIN Qing-chao
A1 - DONG Hai-tao
A1 - PENG You-liang
A1 - CHEN Bao-shan
A1 - SHAO Jing
A1 - DENG Ye
A1 - DAI Cheng-en
A1 - FANG Yong-qi
A1 - LOU Yi-chun
A1 - LI You-zhi
A1 - LI De-bao
J0 - Journal of Zhejiang University Science B
VL - 8
IS - 2
SP - 88
EP - 97
%@ 1673-1581
Y1 - 2007
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2007.B0088


Abstract: 
Appressorium is an infection structure of the phytopathogenic fungus Magnaporthe grisea. Analysis of gene expression profiles of appressorium development provides insight into the molecular basis of pathogenicity and control of this fungal plant disease. A cDNA array representing 2927 unique genes based on a large EST (expressed sequence tag) database of M. grisea strain Y34 was constructed and used to profile the gene expression patterns at mycelium and appressorium maturation stages. Compared with mycelia, 55 up-regulated and 22 down-regulated genes were identified in mature appressoria. Among 77 genes, 16 genes showed no similarity to the genome sequences of M. grisea. A novel homologue of peptidyl-prolyl cis-trans isomerase was found to be expressed at low-level in mature appressoria of M. grisea. The results indicated that the genes such as pyruvate carboxylase, phospholipid metabolism-related protein and glyceraldehyde 3-phosphate dehydrogenase involved in gluconeogenesis, lipid metabolism and glycolysis, showed differential expression in mature appressoria. Furthermore, genes such as PTH11, beta subunit of G protein and SGT1 involved in cell signalling, were expressed differentially in mature appressoria. Northern blot analysis was used to confirm the cDNA array results.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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