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Journal of Zhejiang University SCIENCE B 2007 Vol.8 No.2 P.136~146


Ex vivo expansion and pluripotential differentiation of cryopreserved human bone marrow mesenchymal stem cells

Author(s):  XIANG Ying, ZHENG Qiang, JIA Bing-bing, HUANG Guo-ping, Xu Yu-lin, WANG Jin-fu, PAN Zhi-jun

Affiliation(s):  School of Life Sciences, Zhejiang University, Hangzhou 310012, China; more

Corresponding email(s):   wjfu@zju.edu.cn, zepzj@163.com

Key Words:  Bone marrow mesenchymal stem cells, Cryopreservation, Expansion, Differentiation

XIANG Ying, ZHENG Qiang, JIA Bing-bing, HUANG Guo-ping, Xu Yu-lin, WANG Jin-fu, PAN Zhi-jun. Ex vivo expansion and pluripotential differentiation of cryopreserved human bone marrow mesenchymal stem cells[J]. Journal of Zhejiang University Science B, 2007, 8(2): 136~146.

@article{title="Ex vivo expansion and pluripotential differentiation of cryopreserved human bone marrow mesenchymal stem cells",
author="XIANG Ying, ZHENG Qiang, JIA Bing-bing, HUANG Guo-ping, Xu Yu-lin, WANG Jin-fu, PAN Zhi-jun",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Ex vivo expansion and pluripotential differentiation of cryopreserved human bone marrow mesenchymal stem cells
%A ZHENG Qiang
%A JIA Bing-bing
%A HUANG Guo-ping
%A Xu Yu-lin
%A WANG Jin-fu
%A PAN Zhi-jun
%J Journal of Zhejiang University SCIENCE B
%V 8
%N 2
%P 136~146
%@ 1673-1581
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.B0136

T1 - Ex vivo expansion and pluripotential differentiation of cryopreserved human bone marrow mesenchymal stem cells
A1 - XIANG Ying
A1 - ZHENG Qiang
A1 - JIA Bing-bing
A1 - HUANG Guo-ping
A1 - Xu Yu-lin
A1 - WANG Jin-fu
A1 - PAN Zhi-jun
J0 - Journal of Zhejiang University Science B
VL - 8
IS - 2
SP - 136
EP - 146
%@ 1673-1581
Y1 - 2007
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2007.B0136

This study is aimed at investigating the potentials of ex vivo expansion and pluri-differentiation of cryopreservation of adult human bone marrow mesenchymal stem cells (hMSCs) into chondrocytes, adipocytes and neurocytes. Cryopreserved hMSCs were resuscitated and cultured for 15 passages, and then induced into chondrocytes, adipocytes and neurocytes with corresponding induction medium. The induced cells were observed for morphological properties and detected for expressions of type II collagen, triglyceride or neuron-specific enolase and nestin. The result showed that the resuscitated cells could differentiate into chondrocytes after exposure to transforming growth factor β1 (TGF-β1), insulin-like growth factor I (IGF-I) and vitamin C (VC), and uniformly changed morphologically from a spindle-like fibroblastic appearance to a polygonal shape in three weeks. The induced cells were heterochromatic to safranin O and expressed cartilage matrix-procollagenal (II) mRNA. The resuscitated cells cultured in induction medium consisting of dexamethasone, 3-isobutyl-1-methylxanthine, indomethacin and IGF-I showed adipogenesis, and lipid vacuoles accumulation was detectable after 21 d. The resuscitated hMSCs were also induced into neurocytes and expressed nestin and neuron specific endolase (NSE) that were special surface markers associated with neural cells at different stage. This study suggested that the resuscitated hMSCs should be still a population of pluripotential cells and that it could be used for establishing an abundant hMSC reservoir for further experiment and treatment of various clinical diseases.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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