Full Text:   <3273>

CLC number: Q939.91; S852.4

On-line Access: 2024-08-27

Received: 2023-10-17

Revision Accepted: 2024-05-08

Crosschecked: 0000-00-00

Cited: 11

Clicked: 6732

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2007 Vol.8 No.5 P.331-337

http://doi.org/10.1631/jzus.2007.B0331


Improvement of the efficacy of influenza vaccination (H5N1) in chicken by using extract of Cochinchina momordica seed (ECMS)


Author(s):  RAJPUT Zahid Iqbal, XIAO Chen-wen, HU Song-hua, ARIJO Abdullah G., SOOMRO Noor Mohammad

Affiliation(s):  Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   rao_rajput2002@yahoo.com, songhua@zju.edu.cn

Key Words:  Cochinchina momordica, ECMS, Adjuvant, Chicken, Avian influenza (H5N1), Bird flue


RAJPUT Zahid Iqbal, XIAO Chen-wen, HU Song-hua, ARIJO Abdullah G., SOOMRO Noor Mohammad. Improvement of the efficacy of influenza vaccination (H5N1) in chicken by using extract of Cochinchina momordica seed (ECMS)[J]. Journal of Zhejiang University Science B, 2007, 8(5): 331-337.

@article{title="Improvement of the efficacy of influenza vaccination (H5N1) in chicken by using extract of Cochinchina momordica seed (ECMS)",
author="RAJPUT Zahid Iqbal, XIAO Chen-wen, HU Song-hua, ARIJO Abdullah G., SOOMRO Noor Mohammad",
journal="Journal of Zhejiang University Science B",
volume="8",
number="5",
pages="331-337",
year="2007",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2007.B0331"
}

%0 Journal Article
%T Improvement of the efficacy of influenza vaccination (H5N1) in chicken by using extract of Cochinchina momordica seed (ECMS)
%A RAJPUT Zahid Iqbal
%A XIAO Chen-wen
%A HU Song-hua
%A ARIJO Abdullah G.
%A SOOMRO Noor Mohammad
%J Journal of Zhejiang University SCIENCE B
%V 8
%N 5
%P 331-337
%@ 1673-1581
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.B0331

TY - JOUR
T1 - Improvement of the efficacy of influenza vaccination (H5N1) in chicken by using extract of Cochinchina momordica seed (ECMS)
A1 - RAJPUT Zahid Iqbal
A1 - XIAO Chen-wen
A1 - HU Song-hua
A1 - ARIJO Abdullah G.
A1 - SOOMRO Noor Mohammad
J0 - Journal of Zhejiang University Science B
VL - 8
IS - 5
SP - 331
EP - 337
%@ 1673-1581
Y1 - 2007
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2007.B0331


Abstract: 
Seeds of a Chinese traditional medicine plant, Cochinchina momordica were used in the present study for the improvement of influenza vaccine (H5N1) in chicken. Crude extraction from Cochinchina momordica seed (ECMS) was obtained by ethanol extraction method. In experiment No. 1, two weeks old chickens were immunized with influenza vaccine (H5N1) alone or combined with ECMS (5, 10, 20, 40 and 80 μg/dose). Serum IgG antibody levels (by ELISA) as well as effects on daily weight gain were measured on 0, 7, 14 and 28th day after immunization. Results revealed that all ECMS groups numerically increased the antibody levels while 10 and 20 μg/dose groups significantly (P<0.05) enhanced total IgG antibody on day 28, when compared with control. Average daily weight gain was also significantly higher in 20 μg/dose ECMS group. adjuvant effect was also confirmed in experiment No. 2 when chickens were immunized with 20 μg/dose ECMS and antibody titer was measured through hemagglutination inhibition (HI). It is concluded that ECMS has potential to improve the immune responses and deserve further study as an adjuvant.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1] Alexander, D.J., 2000. A review of avian influenza in different bird species. Vet. Microbiol., 74(1-2):3-13.

[2] Alexander, D.J., Brown, I.H., 2000. Recent zoonoses caused by influenza A viruses. Rev. Sci. Tech., 19(1):197-225.

[3] Chen, X.M., Xu, Y.J., Tian, G.Y., 2005. Physical-chemical properties and structure elucidation of abPS isolated from the root of Achyranthes bidentata. Acta Pharm. Sin., 40(1):32-35.

[4] Easterday, B.C., Hinshaw, V.S., Halvorson, D.A., 1997. Influenza. In: Calnek, B.W., Barnes, H.J., Beard, C.W., McDougald, L.R., Saif, Y.M. (Eds.), Diseases of Poultry. Iowa State University Press, Ames, p.583-605.

[5] Edelman, R., Tacket, C.O., 1990. Adjuvants. Int. Rev. Immunol., 7(1):51-66.

[6] Francis, G., Kerem, Z., Makkar, H.P.S., Becker, K., 2002. The biological action of saponins in animal system: a review. British J. Nutr., 88(6):587-605.

[7] Fritz, J.H., Brunner, S., Birnstiel, M.L., Buschle, M., Gabain, A., Mattner, F., Zauner, W., 2004. The artificial antimicrobial peptide KLKLLLLLKLK induces predominantly a Th2-type immune response to co-injected antigens. Vaccine, 22(25-26):3274-3284.

[8] Gan, M.H., 2004. Avian Influenza, 2nd Ed. Chinese Agricultural Press, Beijing, China, p.112-117.

[9] Gao, X.M., 2005. Mu Bie Zi (Semen momordicae). Chinese Materia Medicia. China Traditional Chinese Materia Medica Press, Beijing, China, p.601-602.

[10] Halman, C., 1993. New Approaches to Vaccine Delivery. In: Hannoun, C., Kendal, A.P., Klenk, H.D., Ruben, F.L. (Eds.), Options for the Control of Influenza, Volume II. Excerpta Medica, Amsterdam, p.389-391.

[11] Haridas, V., Hanausek, M., Nishimura, G., Soehnge, H., Gaikwad, A., Narog, M., Spears, E., Zoltaszek, R., Walaszek, Z., Gutterman, J.U., 2004. Triterpenoid electrophiles (avicins) activate the innate stress response by redox regulation of a gene battery. J. Clin. Invest., 113(1):65-73.

[12] Hu, S., Concha, C., Johannisson, A., Meglia, G., Waller, K.P., 2001. Effect of subcutaneous injection of ginseng on cows with sub-clinical Staphylococcus aureus mastitis. J. Vet. Med. B, 48(7):519-528.

[13] Huang, Z.X., Huang, T., Liu, C.B., 1990. Medical Virology Base and Fundamental Techniques and Methods. Science Press, Beijing, China, p.150-164.

[14] Iwamoto, M., Okabe, H., Yamauchi, T., Tanaka, M., Rokutani, Y., Hara, S., Mihashi, K., Higuchi, R., 1985. Studies on the constituents of Momordica cochinchinensis Spreng I. Isolation and characterization on the seed saponins, momordica saponins I and II. Chem. Pharm. Bull., 33(2):464-478.

[15] Kong, X., Hu, Y., Rui, R., Wang, D., Li, X., 2004. Effects of Chinese herbal medicinal ingredients on peripheral lymphocyte proliferation and serum antibody titer after vaccination in chicken. Int. Immunopharmacol., 4(7):975-982.

[16] Li, C.C., Hu, X.G., Zhang, W.X., Xie, L.W., Zhang, H,Y., Dong, L., Cai, X.H., Wu, R.X., Zhang, Z.X., He, Q.S., 2003. Eosinophils apoptosis, fas mRNA and bcl-2 mRNA expressions in asthma model of young rat and effects of Achyranthes bidentata polysaccharides. Chin. J. Pediatr., 41(9):657-660.

[17] Li, J.X., Hareyama, T., Tezuka, Y., Zhang, Y., Miyahara, T., Kadota, S., 2005. Five new oleanolic acid glycosides from Achyranthes bidentata with inhibitory activity on osteoclast formation. Planta Med., 71(7):673-679.

[18] McCauley, J.W., Pullen, L.A., Forsyth, M., Penn, C.R., Thomas, G.P., 1995. 4-Guanidino-NeuAc2en fails to protect chickens from infection with highly pathogenic avian influenza virus. Antivir. Res., 27(1-2):179-186.

[19] McElrath, M.J., 1995. Selection of potent immunological adjuvants for vaccine construction. Semin. Cancer Biol., 6(6):375-385.

[20] Normile, D., 2005. North Korea collaborates to fight bird flu. Science, 308(5719):175.

[21] Oda, K., Matsuda, H., Murakami, T., Katayama, S., Ohgitani, T., Yoshikawa, M., 2000. Adjuvant and haemolytic activities of 47 saponins derived from medicinal and food plants. Biol. Chem., 381(1):67-74.

[22] Palese, P., 2006. Making better influenza virus vaccines? Emerg. Infect. Dis., 12(1):61-65. http://www.cdc.gov/eid

[23] Rimmelzwaan, G.F., Osterhaus, A.D., 2001. Influenza vaccine: new developments. Curr. Opin. Pharmacol., 1(5):491-496.

[24] Rivera, E., Ekholm, P.F., Inganas, M., Paulie, S., Gronvik, K.O., 2005. The Rb1 fraction of ginseng elicits a balanced Th1 and Th2 immune response. Vaccine, 23(46-47):5411-5419.

[25] Sambhara, S., Kurichh, A., Miranda, R., Tumpey, T., Rowe, T., Renshaw, M., Arpino, R., Tamane, A., Kandil, A., James, O., et al., 2001. Heterosubtypic immunity against human influenza A viruses, including recently emerged avian H5 and H9 viruses, induced by FLU-ISCOM vaccine in mice requires both cytotoxic T-lymphocyte and macrophage function. Cell. Immunol., 211(2):143-153.

[26] Sheng, Y.X., Jun, W.L., Zhou, W.J., 2003. Natural Medicine Chemistry. Triterpenoid Saponins (Chapter 7), Extraction and Separation. People Hygiene Publisher, Beijing, China, p.294 (in Chinese).

[27] Suarez, D.L., Schultz-Cherry, S., 2000. Immunology of avian influenza virus: a review. Dev. Comp. Immunol., 24(2-3):269-283.

[28] Swayne, D.E., Senne, D.A., Beard, C.W., 1998. Avian Influenza. In: Swayne, D.E. (Ed.), A Laboratory Manual for the Isolation and Identification of Avian Pathogens. American Association of Avian Pathologists, Kennett Square, Pa, p.150-155.

[29] Wang, M., Guilbert, L.J., Ling, L., Li, J., Wu, Y., Xu, S., Pang, P., Shan, J.J., 2001. Immunomodulating activity of CTV-E002, a proprietary extract from North American ginseng (Panax equinquefolium). J. Pharm. Pharmacol., 53(11):1515-1523.

[30] Yang, Z.G., Sun, H.X., Fang, W.H., 2005. Haemolytic activities and adjuvant effect of Astragalus membranaceus saponins (AMS) on the immune responses to ovalbumin in mice. Vaccine, 23(44):5196-5203.

[31] Zheng, S., Li, G.E., Yan, S.M., 1992. Purification and characterisation of momordin. Acta Biochim. Biophys. Sin., 24:311-316 (in Chinese).

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE