Full Text:   <2173>

CLC number: Q93; R93

On-line Access: 

Received: 2006-11-05

Revision Accepted: 2007-09-10

Crosschecked: 0000-00-00

Cited: 0

Clicked: 3513

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2007 Vol.8 No.11 P.845~852

10.1631/jzus.2007.B0845


Enhanced production of elastase by Bacillus licheniformis ZJUEL31410: optimization of cultivation conditions using response surface methodology


Author(s):  CHEN Qi-he, RUAN Hui, ZHANG Hai-feng, NI Hui, HE Guo-qing

Affiliation(s):  Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   ruanhui98@yahoo.com.cn, gqhe@zju.edu.cn

Key Words:  Elastase, Bacillus licheniformis ZJUEL31410, Cultivation condition, Fractional factorial design (FFD), Response surface methodology


Share this article to: More <<< Previous Article|

CHEN Qi-he, RUAN Hui, ZHANG Hai-feng, NI Hui, HE Guo-qing. Enhanced production of elastase by Bacillus licheniformis ZJUEL31410: optimization of cultivation conditions using response surface methodology[J]. Journal of Zhejiang University Science B, 2007, 8(11): 845~852.

@article{title="Enhanced production of elastase by Bacillus licheniformis ZJUEL31410: optimization of cultivation conditions using response surface methodology",
author="CHEN Qi-he, RUAN Hui, ZHANG Hai-feng, NI Hui, HE Guo-qing",
journal="Journal of Zhejiang University Science B",
volume="8",
number="11",
pages="845~852",
year="2007",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2007.B0845"
}

%0 Journal Article
%T Enhanced production of elastase by Bacillus licheniformis ZJUEL31410: optimization of cultivation conditions using response surface methodology
%A CHEN Qi-he
%A RUAN Hui
%A ZHANG Hai-feng
%A NI Hui
%A HE Guo-qing
%J Journal of Zhejiang University SCIENCE B
%V 8
%N 11
%P 845~852
%@ 1673-1581
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.B0845

TY - JOUR
T1 - Enhanced production of elastase by Bacillus licheniformis ZJUEL31410: optimization of cultivation conditions using response surface methodology
A1 - CHEN Qi-he
A1 - RUAN Hui
A1 - ZHANG Hai-feng
A1 - NI Hui
A1 - HE Guo-qing
J0 - Journal of Zhejiang University Science B
VL - 8
IS - 11
SP - 845
EP - 852
%@ 1673-1581
Y1 - 2007
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2007.B0845


Abstract: 
Sequential methodology based on the application of three types of experimental designs was used to optimize the fermentation conditions for elastase production from mutant strain ZJUEL31410 of Bacillus licheniformis in shaking flask cultures. The optimal cultivation conditions stimulating the maximal elastase production consist of 220 r/min shaking speed, 25 h fermentation time, 5% (v/v) inoculums volume, 25 ml medium volume in 250 ml Erlenmeyer flask and 18 h seed age. Under the optimized conditions, the predicted maximal elastase activity was 495 U/ml. The application of response surface methodology resulted in a significant enhancement in elastase production. The effects of other factors such as elastin and the growth factor (corn steep flour) on elastase production and cell growth were also investigated in the current study. The elastin had no significant effect on enzyme-improved production. It is still not clear whether the elastin plays a role as a nitrogen source or not. Corn steep flour was verified to be the best and required factor for elastase production and cell growth by Bacillus licheniformis ZJUEL31410.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1] Box, G.E.P., Wilson, K.B., 1951. On the experimental attainment of optimum conditions. J. Roy Stat. Soc. B, 13:145.

[2] Box, G.E.P., Hunter, W.G., 1978. Statistics for Experimenters. John Wiley and Sons, NY.

[3] Box, G.E.P., Draper, N.R., 1987. Empirical Model-Building and Response Surfaces. John Wiley and Sons, NY, p.669.

[4] Chen, Q.H., He, G.Q., Mokhtar, A.M.A., 2002. Optimization of medium composition for the production of elastase by Bacillus sp. EL31410 with response surface methodology. Enzyme Microb. Technol., 30(5):667-672.

[5] Chen, Q.H., He, G.Q., Schwarz, P., 2004. Studies on cultivation kinetics for elastase production by Bacillus sp. EL31410. J. Agric. Food Chem., 52(11):3356-3359.

[6] Clark, D.J., Hawrylik, S.J., Kavanagh, E., Opheim, D.J., 2000. Purification and characterization of a unique alkaline elastase from Micrococcus luteus. Protein Expr. Purif., 18(1):46-55.

[7] de O. Souza, M.C., Roberto, I.C., Milagres, A.M.F., 1999. Solid-state fermentation for xylanase production by Thermoascus aurantiacus using response surface methodology. Appl. Microbial. Biotechnol., 52(6):768-772.

[8] Haaland, P.D., 1989. Experimental Design in Biotechnology. Dekker, New York.

[9] He, G.Q., Chen, Q.H., Ju, X.J., Shi, N.D., 2004. Improved elastase production by Bacillus sp. EL31410-further optimization and kinetics studies of culture medium for batch fermentation. J. Zhejiang Univ. Sci., 5(2):149-156.

[10] Janda, J.M., Abbott, S., 1999. Identification and initial characterization of elastase activity associated with Vibrio cholerae. Curr. Microbiol., 39(2):73-78.

[11] Kalil, S.J., Maugeri, F., Rodrigues, M.I., 2000. Response surface analysis and simulation as a tool for bioprocess design and optimization. Process Biochem., 35(6):539-550.

[12] Kapat, A., Jung, J.K., Park, Y.H., 2001. Enhancement of glucose oxidase production in batch cultivation of recombination Saccharomyces cerevisiae: optimization of oxygen transfer condition. J. Appl. Microbiol., 90(2):216-222.

[13] Khuri, A.I., Cornell, J.A., 1987. Response Surfaces Design and Analysis. Dekker, NY.

[14] King, V.A., 1983. Studies on the control of the growth of Saccharomyces cerevisiae by using response surface methodology to achieve effective preservation at high water activities. Int. J. Food Sci. Technol., 28:519-529.

[15] Ma, A.Y.M., Ooraikul, B., 1986. Optimization of enzymatic hydrolysis of canola meal with response surface methodology. J. Food Proc. Preserv., 10(2):99-113.

[16] Miller, G.L., 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem., 31(3):426-427.

[17] Montgomery, D.C., 1997. Response Surface Methods and Other Approaches to Process Optimization. In: Montgomery, D.C. (Ed.), Design and Analysis of Experiments. John Wiley and Sons, NY, p.427-510.

[18] Morihara, K., 1967. Elastolytic properties of various proteinase from microbial origin. Arch. Biochem. Biophys., 120(1):68-78.

[19] Ozaki, H., Shiio, I., 1975. Purification and properties of elastolytic enzyme from Flavobacterium immotum. J. Biochem., 77:171-180.

[20] Ramírez, J., Gutierrez, H., Gschaedler, A., 2001. Optimization of astaxanthin production by Phaffia rhodozyma through factorial design and response surface methodology. J. Biotechnol., 88(3):259-268.

[21] Roseiro, J.C., 1992. Medium development for xanthan production. Process Biochem., 27(3):167-175.

[22] Rosi, I., Costamagna, L., Bertuccioli, M., 1987. Wine Fermentation by Immobilized Yeast: An Optimization Study. In: Flavor Science and Technology. John Wiley and Sons, NY.

[23] Sacher, L.A., 1955. Photometry method for estimation of elastase activity. Proc. Soc. Exp. Biol. Med., 90:323-325.

[24] Shibata, Y., Fujimura, S., Nakamura, T., 1993. Purification and partial characterization of an elastolytic serine protease of Prevotella intermedia. Appl. Environ. Microbial., 7:2107-2111.

[25] Shiio, I., Nakamatsu, T., Ozaki, H., 1974. Microbial production of elastolytic enzymes. Agri. Biol. Chem., 1:1-7.

[26] Tsai, Y.C.H., Jung, R.Y., Lin, S.F., 1988. Production and further characterization of an alkaline elastase production by alkalophilic Bacillus strain YaB. Appl. Environ. Microb., 1:3156-3161.

[27] Tsuzuki, H., Oka, T., 1965. Pseudomonas aeruginosa elastase: isolation, crystallization and preliminary characterization. J. Biol. Chem., 8:3295-3303.

[28] Zins, M.M., Zimprich, C.A., Petermann, S.R., Rust, L., 2001. Expression and partial characterization of an elastase from Chromobacterium violaceum. Vet. Microbiol., 80(1):63-74.

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE