Full Text:   <1824>

CLC number: X703

On-line Access: 

Received: 2008-01-02

Revision Accepted: 2008-06-26

Crosschecked: 2008-10-29

Cited: 0

Clicked: 4131

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE A 2009 Vol.10 No.1 P.133~141


Applicability of anoxic-oxic process in treating petrochemical wastewater

Author(s):  Li-jun ZHAO, Fang MA, Jing-bo GUO

Affiliation(s):  School of Chemical Engineering, China University of Petroleum, Beijing 102249, China; more

Corresponding email(s):   wwep001@yahoo.com.cn

Key Words:  Petrochemical wastewater, Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), Anoxic-oxic (A/O) process, Applicability

Li-jun ZHAO, Fang MA, Jing-bo GUO. Applicability of anoxic-oxic process in treating petrochemical wastewater[J]. Journal of Zhejiang University Science A, 2009, 10(1): 133~141.

@article{title="Applicability of anoxic-oxic process in treating petrochemical wastewater",
author="Li-jun ZHAO, Fang MA, Jing-bo GUO",
journal="Journal of Zhejiang University Science A",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Applicability of anoxic-oxic process in treating petrochemical wastewater
%A Li-jun ZHAO
%A Fang MA
%A Jing-bo GUO
%J Journal of Zhejiang University SCIENCE A
%V 10
%N 1
%P 133~141
%@ 1673-565X
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.A0820006

T1 - Applicability of anoxic-oxic process in treating petrochemical wastewater
A1 - Li-jun ZHAO
A1 - Fang MA
A1 - Jing-bo GUO
J0 - Journal of Zhejiang University Science A
VL - 10
IS - 1
SP - 133
EP - 141
%@ 1673-565X
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.A0820006

To explore the applicability of anoxic-oxic (A/O) activated sludge process for petrochemical wastewater treatment, the relationship between bacterial community structure and pollutants loading/removal efficiencies was investigated by gas chromatograph-mass spectrometry (GC-MS), polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and other conventional techniques. It showed that when the concentrations of the influent chemical oxygen demand (COD) and ammonia nitrogen (NH4+-N) were 420~560 mg/L and 64~100 mg/L, respectively, the corresponding average effluent concentrations were 160 mg/L and 55 mg/L, which were 1.6 and 2.2 times higher than those of the national standards in China, respectively, demonstrating the inefficient performances of A/O process. Analysis of GC-MS indicated that refractory pollutants were mainly removed by sludge adsorption, but not by biodegradation. PCR-DGGE profile analysis suggested that the biological system was species-rich, but there was apparent succession of the bacterial community structure in different locations of the A/O system. Variations of bacterial community structure and pollutant loadings had obvious influences on pollutants removal efficiencies. Thus, A/O process was inapplicable for the treatment of complicated petrochemical wastewater, and strategies such as the reinforcement of pre-treatment and two-stage A/O process were suggested.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Buchholz-Cleven, B.E.E., Rattunde, B., Straub, K.L., 1997. Screening for genetic diversity of isolates of anaerobic Fe(II)-oxidizing bacteria using DGGE and whole cell hybridization. Systematic and Applied Microbiology, 20:301-309.

[2] Eichner, C.A., Erb, R.W., Timmis, K.N., 1999. Thermal gradient gel electrophoresis analysis of bioprotection from pollutant shocks in the activated sludge microbial community. Applied and Environmental Microbiology, 65(1):102-109.

[3] Gao, P.P., Zhao, Y., Zhao, L.P., 2003. Analysis of the microbial community of activated sludge in different aeration basins within an industrial phenol remediation system by ERIC-PCR fingerprinting. Acta Scientiae Circumstantiae, 23(6):705-710 (in Chinese).

[4] Hansen, M.C., Tolkernielsen, T., Givskov, M., Molin, S., 1998. Biased 16S rDNA PCR amplification caused by interference from DNA flanking the template region. FEMS Microbiology Ecology, 26(2):141-149.

[5] Lapara, T.M., Nakatsu, C.H., Pantea, L.M., Alleman, J.E., 2002. Stability of the bacterial communities supported by a seven-stage biological process treating pharmaceutical wastewater as revealed by PCR-DGGE. Water Research, 36(3):638-646.

[6] Ling, W.H, Xiao, Y.C., 2003. Application of up-flow anaerobic sludge bed in the pretreatment of high concentrated wastewater of petroleum chemical production. Shanghai Chemical Industry, 8:7-10 (in Chinese).

[7] Liu, G.L., Chong, Y.X., Fan, Q.J., Jia, X.S., Li, S.H., 2006. Hydrodynamic effects of the oxidation ditch on the removal efficiency and energy consumption. Environment Science, 27(11):2323-2326 (in Chinese).

[8] Margesin, R., Schinner, F., 1999. Biodegradation of diesel oil by cold-adapted microorganisms in presence of sodium dodecyl sulfate. Chemosphere, 38(15):3463-3472.

[9] Marsh, T.L., Liu, W.T., Forney, L.J., Cheng, H., 1998. Beginning a molecular analysis of the eukaryal community in activated sludge. Water Science and Technology, 37(4-5):455-460.

[10] Myers, R.M., 1985. Modification of the melting properties of duplex DNA by attachment of a GC-rich DNA sequence as determined by denaturing gradient gel electrophoresis. Nucleic Acids Research, 13(9):3111-3129.

[11] Nyholm, N., 1996. Biodegradability characterization of mixtures of chemical contaminants in wastewater—the utility of biotests. Water Science and Technology, 33(6):195-206.

[12] Orphan, V.J., Taylor, L.T., Hafenbradl, D., Delong, E.F., 2000. Culture-dependent and culture-independent characterization of microbial assemblages associated with high-temperature petroleum reservoirs. Applied and Environmental Microbiology, 66(2):700-711.

[13] Patel, H., Madamwar, D., 2002. Effects of temperatures and organic loading rates on biomethanation of acidic petrochemical wastewater using an anaerobic upflow fixed-film reactor. Bioresource Technology, 82(1):65-71.

[14] Ren, N.Q., Ma, F., 2003.Theory and Application of Microbiology for Pollution Control. Chemical Industry Press, Beijing, p.64-68 (in Chinese).

[15] SEPAC (State Environmental Protection Administration of China), 2002. Water and Wastewater Analytical Methods (4Ed.). China Environmental Press, Beijing (in Chinese).

[16] Shi, L.J., Xun, Y.X., Yang, F.L., Zhang, X.W., Wang, X.J., Kenji, F., 2006. Biological nitrogen removal from petrochemical wastewater using anoxic and oxic swim-bed reactor. Journal of Chemical Industry and Engineering, 57(1):104-108 (in Chinese).

[17] SSB (State Standard Bureau), 2002. Discharge Standard of Pollutants for Municipal Wastewater Treatment Plant GB18918-2002. Standards Press of China, General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China (in Chinese).

[18] Wen, Y., Huang, X.F., Qiu Z., Wang F., Zhang, F.J., Zhou, Q., 2006. Experimental study on the mechanism of oilfield wastewater treatment by using hydrolysis-acidification with aerobic biological process. Chinese Journal of Environmental Science, 27(7):1362-1368 (in Chinese).

[19] Xue, L.H., 2002. Transfer and transformation of the organic compound in the process of treating municipal wastewater. Journal of Safety and Environment, 2(5):45-47 (in Chinese).

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE