Full Text:   <1836>

CLC number: TB611; R331.1

On-line Access: 

Received: 2008-07-09

Revision Accepted: 2008-09-04

Crosschecked: 2009-02-25

Cited: 2

Clicked: 3730

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE A 2009 Vol.10 No.5 P.697~703


Experimental study on rehydration conditions of freeze-dried platelets

Author(s):  Ju-li FAN, Xian-guo XU, Shao-zhi ZHANG, Fa-ming ZHU, Guang-ming CHEN, Li-xing YAN

Affiliation(s):  Institute of Refrigeration and Cryogenics, Zhejiang University, Hangzhou 310027, China; more

Corresponding email(s):   enezsz@zju.edu.cn

Key Words:  Freeze-drying, Platelets, Rehydration, Prehydration, Rehydration solution

Ju-li FAN, Xian-guo XU, Shao-zhi ZHANG, Fa-ming ZHU, Guang-ming CHEN, Li-xing YAN. Experimental study on rehydration conditions of freeze-dried platelets[J]. Journal of Zhejiang University Science A, 2009, 10(5): 697~703.

@article{title="Experimental study on rehydration conditions of freeze-dried platelets",
author="Ju-li FAN, Xian-guo XU, Shao-zhi ZHANG, Fa-ming ZHU, Guang-ming CHEN, Li-xing YAN",
journal="Journal of Zhejiang University Science A",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Experimental study on rehydration conditions of freeze-dried platelets
%A Ju-li FAN
%A Xian-guo XU
%A Shao-zhi ZHANG
%A Fa-ming ZHU
%A Guang-ming CHEN
%A Li-xing YAN
%J Journal of Zhejiang University SCIENCE A
%V 10
%N 5
%P 697~703
%@ 1673-565X
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.A0820549

T1 - Experimental study on rehydration conditions of freeze-dried platelets
A1 - Ju-li FAN
A1 - Xian-guo XU
A1 - Shao-zhi ZHANG
A1 - Fa-ming ZHU
A1 - Guang-ming CHEN
A1 - Li-xing YAN
J0 - Journal of Zhejiang University Science A
VL - 10
IS - 5
SP - 697
EP - 703
%@ 1673-565X
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.A0820549

A rehydration process for freeze-dried human platelets was studied on 1 ml of samples. The effects of prehydration duration, prehydration temperature, and rehydration solution on the recovery rate, mean platelet volume (MPV), and platelet distribution width (PDW) were investigated. The mass changes during the prehydration process were also studied. Three prehydration durations: 0, 1.5, and 3.5 h, and two rehydration solutions: platelet-poor plasma and phosphate-buffered saline (PBS), were tested. It was found that: (1) the prehydration was of significance; (2) 1.5 h of prehydration had better effects than 3.5 h of prehydration; (3) as a rehydration solution, the platelet-poor plasma behaved better than the PBS. The impacts of prehydration duration and temperature on the results were studied. There was almost no difference between 35 and 37 °C. Among all the prehydration durations tested, 15, 30, 60, 90, and 120 min, the best result was achieved with the time duration of 15 min. The weights of prehydrated platelets at the end of each test were measured and the water contents were calculated. After 15 min of prehydration, the water contents in the samples were about (4.8±0.01)% and (5.27±0.29)% (w/w) corresponding to the conditions of 35 and 37 °C, respectively. These results will be helpful for further studies on the freeze-drying of mammalian cells.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Acker, J.P., Fowler, A., Lauman, B., Cheley, S., Toner, M., 2002. Survival of desiccated mammalian cells: beneficial effects of isotonic media. Cell Preservation Technology, 1(2):129-140.

[2] Bhanot, S., Alex, J.C., 2002. Current applications of platelet gels in facial plastic surgery. Facial Plastic Surgery, 18(1):27-34.

[3] Bock, M., Schleuning, M., Heim, M.U., Wempel, W., 1995. Cryopreservation of human platelets with dimethylsulfoxide: Changes in biochemistry and cell function. Transfusion, 35(11):921-924.

[4] Bode, A.P., Lust, R.M., Read, M.S., Fischer, T.H., 2008. Correction of the bleeding time with lyophilized platelet infusions in dogs on cardiopulmonary bypass. Clinical and Applied Thrombosis/Hemostasis, 14(1):38-54.

[5] Chen, H., 2006. The assay sense of MPV, PCT and PDW of hemophiles’ platelets. Applied Journal of General Practice, 4(4):479 (in Chinese).

[6] Han, Y., Quan, G.B., Liu, X.Z., 2004. Efect of reconstitution solutions on the recovery of lyophilized red blood cells. Chin. J. Blood Transfusion, 17(1):10-12 (in Chinese).

[7] Kim, S.G., Chung, C.H., Kim, Y.K., Park, J.C., Lim, S.C., 2002. Use of particulate dentin-plaster of Paris combination with/without platelet-rich plasma in the treatment of bone defects around implants. The International Journal of Oral & Maxillofacial Implants, 17(1):86-94.

[8] Liu, J.H., Lv, A.B., 2007. Diabetes patient blood platelet parameter level observation and preliminary analysis. Journal of Qiqihar Medical College, 28(24):2954-2955 (in Chinese).

[9] Liu, J.H., Zhou, J., Wang, D.M., Ouyang, X.L., Xing, Y.C., Lu, F.Q., 2007. Experimental study on lyophilization of platelets. Zhongguo Shi Yan Xue Ye Xue Za Zhi, 15(5):1098-1101 (in Chinese).

[10] Marx, R.E., Carlson, E.R., Eichstaedt, R.M., Schimmele, S.R., Strauss, J.E., Georgeff, K.R., 1998. Platelet-rich plasma: growth factor enhancement for bone grafts. Oral Surgery, Oral Medicine, Oral Pathology Oral Radiology, and Endodontology, 85(6):638-646.

[11] Read, M.S., Reddick, R.L., Bode, A.P., Bellinger, D.A., Nichols, T.C., Taylor, K., Smith, S.V., McMahon, D.C., Griggs, T.R., Brinkhous, K.M., 1995. Preservation of hemostatic and structural properties of rehydrated lyophilized platelets: potential for long-term storage of dried platelets for transfusion. Proceedings of the National Academy of Sciences, 92(2):397-401.

[12] Shu, Z.Q., Liu, Z., Chen, W.W., He, L.Q., Gao, D.Y., 2007. The Rehydration Injury Study of Freeze-dried Red Blood Cells during Rehydration. International Congress of Refrigeration, Beijing.

[13] Tablin, F., Oliver, A., Walker, N.I., Crowe, L.M., Crowe, J.H., 1996. Membrane phase transition of intact human platelets: correlation with cold-induced activation. Journal of Cellular Physiology, 168(2):305-313.

[14] Tablin, F., Wolkers, W.F., Walker, N.J., Oliver, A.E., Tsvetkova, N.M., Gousset, K., Crowe, L.M., Crowe, J.H., 2001. Membrane reorganization during chilling: implications for long-term stabilization of platelets. Cryobiology, 43(2):114-123.

[15] Wolkers, W.F., Walker, N.J., Tablin, F., Crowe, J.H., 2001. Human platelets loaded with trehalose survive freeze-drying. Cryobiology, 42(2):79-87.

[16] Wolkers, W.F., Walker, N.J., Tamari, Y., Tablin, F., Crowe, J.H., 2002. Towards a clinical application of freeze-dried human platelets. Cell Preservation Technology, 1(3):175-188.

[17] Zhou, X.L., Zhu, H., Zhang, S.Z., Zhu, F.M., Chen, G.M., Yan, L.X., 2007. Freeze-drying of human platelets: influence of saccharide, freezing rate and cell concentration. Cryo Letters, 28(3):187-196.

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE