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Journal of Zhejiang University SCIENCE A 2009 Vol.10 No.5 P.697~703

http://doi.org/10.1631/jzus.A0820549


Experimental study on rehydration conditions of freeze-dried platelets


Author(s):  Ju-li FAN, Xian-guo XU, Shao-zhi ZHANG, Fa-ming ZHU, Guang-ming CHEN, Li-xing YAN

Affiliation(s):  Institute of Refrigeration and Cryogenics, Zhejiang University, Hangzhou 310027, China; more

Corresponding email(s):   enezsz@zju.edu.cn

Key Words:  Freeze-drying, Platelets, Rehydration, Prehydration, Rehydration solution


Ju-li FAN, Xian-guo XU, Shao-zhi ZHANG, Fa-ming ZHU, Guang-ming CHEN, Li-xing YAN. Experimental study on rehydration conditions of freeze-dried platelets[J]. Journal of Zhejiang University Science A, 2009, 10(5): 697~703.

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author="Ju-li FAN, Xian-guo XU, Shao-zhi ZHANG, Fa-ming ZHU, Guang-ming CHEN, Li-xing YAN",
journal="Journal of Zhejiang University Science A",
volume="10",
number="5",
pages="697~703",
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publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.A0820549"
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%T Experimental study on rehydration conditions of freeze-dried platelets
%A Ju-li FAN
%A Xian-guo XU
%A Shao-zhi ZHANG
%A Fa-ming ZHU
%A Guang-ming CHEN
%A Li-xing YAN
%J Journal of Zhejiang University SCIENCE A
%V 10
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%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.A0820549

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T1 - Experimental study on rehydration conditions of freeze-dried platelets
A1 - Ju-li FAN
A1 - Xian-guo XU
A1 - Shao-zhi ZHANG
A1 - Fa-ming ZHU
A1 - Guang-ming CHEN
A1 - Li-xing YAN
J0 - Journal of Zhejiang University Science A
VL - 10
IS - 5
SP - 697
EP - 703
%@ 1673-565X
Y1 - 2009
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.A0820549


Abstract: 
A rehydration process for freeze-dried human platelets was studied on 1 ml of samples. The effects of prehydration duration, prehydration temperature, and rehydration solution on the recovery rate, mean platelet volume (MPV), and platelet distribution width (PDW) were investigated. The mass changes during the prehydration process were also studied. Three prehydration durations: 0, 1.5, and 3.5 h, and two rehydration solutions: platelet-poor plasma and phosphate-buffered saline (PBS), were tested. It was found that: (1) the prehydration was of significance; (2) 1.5 h of prehydration had better effects than 3.5 h of prehydration; (3) as a rehydration solution, the platelet-poor plasma behaved better than the PBS. The impacts of prehydration duration and temperature on the results were studied. There was almost no difference between 35 and 37 °C. Among all the prehydration durations tested, 15, 30, 60, 90, and 120 min, the best result was achieved with the time duration of 15 min. The weights of prehydrated platelets at the end of each test were measured and the water contents were calculated. After 15 min of prehydration, the water contents in the samples were about (4.8±0.01)% and (5.27±0.29)% (w/w) corresponding to the conditions of 35 and 37 °C, respectively. These results will be helpful for further studies on the freeze-drying of mammalian cells.

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