Full Text:   <1298>

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CLC number: X592

On-line Access: 2014-08-04

Received: 2014-06-02

Revision Accepted: 2014-06-22

Crosschecked: 2014-07-18

Cited: 8

Clicked: 3499

Citations:  Bibtex RefMan EndNote GB/T7714

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Journal of Zhejiang University SCIENCE A 2014 Vol.15 No.8 P.618-623


Random amplified polymorphic DNA reveals that TiO2 nanoparticles are genotoxic to Cucurbita pepo *

Author(s):  Fabiola Moreno-Olivas1,4, Vincent U. Gant Jr.2, Kyle L. Johnson2, Jose R. Peralta-Videa1,3,4, Jorge L. Gardea-Torresdey1,3,4

Affiliation(s):  1. Depertment of Chemistry, The University of Texas at El Paso, El Paso, TX 79968, USA; more

Corresponding email(s):   fmoreno2@miners.utep.edu

Key Words:  Random amplified polymorphic DNA (RAPD), Titanium dioxide (TiO2), Nanoparticles (NPs), Genomic DNA, Zucchini

Fabiola Moreno-Olivas, Vincent U. Gant Jr. , Kyle L. Johnson, Jose R. Peralta-Videa, Jorge L. Gardea-Torresdey. Random amplified polymorphic DNA reveals that TiO2 nanoparticles are genotoxic to Cucurbita pepo[J]. Journal of Zhejiang University Science A, 2014, 15(8): 618-623.

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author="Fabiola Moreno-Olivas, Vincent U. Gant Jr. , Kyle L. Johnson, Jose R. Peralta-Videa, Jorge L. Gardea-Torresdey",
journal="Journal of Zhejiang University Science A",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Random amplified polymorphic DNA reveals that TiO2 nanoparticles are genotoxic to Cucurbita pepo
%A Fabiola Moreno-Olivas
%A Vincent U. Gant Jr.
%A Kyle L. Johnson
%A Jose R. Peralta-Videa
%A Jorge L. Gardea-Torresdey
%J Journal of Zhejiang University SCIENCE A
%V 15
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%P 618-623
%@ 1673-565X
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.A1400159

T1 - Random amplified polymorphic DNA reveals that TiO2 nanoparticles are genotoxic to Cucurbita pepo
A1 - Fabiola Moreno-Olivas
A1 - Vincent U. Gant Jr.
A1 - Kyle L. Johnson
A1 - Jose R. Peralta-Videa
A1 - Jorge L. Gardea-Torresdey
J0 - Journal of Zhejiang University Science A
VL - 15
IS - 8
SP - 618
EP - 623
%@ 1673-565X
Y1 - 2014
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.A1400159

Titanium dioxide nanoparticles (TiO2 NPs) are used in cosmetics, sunscreens, paints, and toothpaste, among other applications. These NPs are very stable and can be transported and dispersed in wastewater and biosolids. Animal species have shown negative reactions to TiO2 NPs. However, little is known about their toxicity in plants, specifically the possibility of genotoxic effects. In this study, we used a random amplified polymorphic DNA (RAPD) technique to study the genotoxic effects of TiO2 NPs on hydroponically cultivated zucchini (Cucurbita pepo) plants. Seeds were allowed to germinate for 7 d and plants were selected at random for individual and population studies. Four plants were selected for the individual study and 18 for the population study. RAPD profiles of TiO2 NPs treated plants showed differences in band intensity, loss of bands, or appearance of new bands, compared to untreated plants. To the authors’ knowledge, this is the first report of the genotoxic potential of TiO2 NPs in zucchini.



Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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