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CLC number: Q81

On-line Access: 2008-01-06

Received: 2007-06-12

Revision Accepted: 2007-10-23

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Cited: 16

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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.2 P.121~131

http://doi.org/10.1631/jzus.B0710307


Sensing Escherichia coli O157:H7 via frequency shift through a self-assembled monolayer based QCM immunosensor


Author(s):  Li-jiang WANG, Chun-sheng WU, Zhao-ying HU, Yuan-fan ZHANG, Rong LI, Ping WANG

Affiliation(s):  Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China

Corresponding email(s):   cnpwang@zju.edu.cn

Key Words:  Biosensor, Escherichia coli O157:H7, Immunosensor, Layer-by-layer self-assembly (LBL-SA), Quartz crystal microbalance (QCM)


Li-jiang WANG, Chun-sheng WU, Zhao-ying HU, Yuan-fan ZHANG, Rong LI, Ping WANG. Sensing Escherichia coli O157:H7 via frequency shift through a self-assembled monolayer based QCM immunosensor[J]. Journal of Zhejiang University Science B, 2008, 9(2): 121~131.

@article{title="Sensing Escherichia coli O157:H7 via frequency shift through a self-assembled monolayer based QCM immunosensor",
author="Li-jiang WANG, Chun-sheng WU, Zhao-ying HU, Yuan-fan ZHANG, Rong LI, Ping WANG",
journal="Journal of Zhejiang University Science B",
volume="9",
number="2",
pages="121~131",
year="2008",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0710307"
}

%0 Journal Article
%T Sensing Escherichia coli O157:H7 via frequency shift through a self-assembled monolayer based QCM immunosensor
%A Li-jiang WANG
%A Chun-sheng WU
%A Zhao-ying HU
%A Yuan-fan ZHANG
%A Rong LI
%A Ping WANG
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 2
%P 121~131
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0710307

TY - JOUR
T1 - Sensing Escherichia coli O157:H7 via frequency shift through a self-assembled monolayer based QCM immunosensor
A1 - Li-jiang WANG
A1 - Chun-sheng WU
A1 - Zhao-ying HU
A1 - Yuan-fan ZHANG
A1 - Rong LI
A1 - Ping WANG
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 2
SP - 121
EP - 131
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0710307


Abstract: 
By means of the specific immuno-recognition and ultra-sensitive mass detection, a quartz crystal microbalance (QCM) biosensor for Escherichia coli O157:H7 detection was developed in this work. As a suitable surfactant, 16-mercaptohexadecanoic acid (MHDA) was introduced onto the Au surface of QCM, and then self-assembled with N-hydroxysuccinimide (NHS) raster as a reactive intermediate to provide an active interface for the specific antibody immobilization. The binding of target bacteria with the immobilized antibodies decreased the sensor’s resonant frequency, and the frequency shift was correlated to the bacterial concentration. The stepwise assembly of the immunosensor was characterized by means of the electrochemical techniques. Using the immersion-dry-immersion procedure, this QCM biosensor could detect 2.0×102 colony forming units (CFU)/ml E. coli O157:H7. In order to reduce the fabrication time, a polyelectrolyte layer-by-layer self-assembly (LBL-SA) method was adopted for fast construction. Finally, the reproducibility of this biosensor was discussed.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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