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Received: 2007-07-20

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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.2 P.148~153


A survey on porcine circovirus type 2 infection and phylogenetic analysis of its ORF2 gene in Hangzhou, Zhejiang Province, China

Author(s):  Zong-zhao YANG, Jiang-bing SHUAI, Xian-jun DAI, Wei-huan FANG

Affiliation(s):  Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   whfang@zju.edu.cn

Key Words:  Porcine circovirus type 2 (PCV2), Seroprevalence, ORF2, Phylogenetic analysis

Zong-zhao YANG, Jiang-bing SHUAI, Xian-jun DAI, Wei-huan FANG. A survey on porcine circovirus type 2 infection and phylogenetic analysis of its ORF2 gene in Hangzhou, Zhejiang Province, China[J]. Journal of Zhejiang University Science B, 2008, 9(2): 148~153.

@article{title="A survey on porcine circovirus type 2 infection and phylogenetic analysis of its ORF2 gene in Hangzhou, Zhejiang Province, China",
author="Zong-zhao YANG, Jiang-bing SHUAI, Xian-jun DAI, Wei-huan FANG",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T A survey on porcine circovirus type 2 infection and phylogenetic analysis of its ORF2 gene in Hangzhou, Zhejiang Province, China
%A Zong-zhao YANG
%A Jiang-bing SHUAI
%A Xian-jun DAI
%A Wei-huan FANG
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 2
%P 148~153
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0710395

T1 - A survey on porcine circovirus type 2 infection and phylogenetic analysis of its ORF2 gene in Hangzhou, Zhejiang Province, China
A1 - Zong-zhao YANG
A1 - Jiang-bing SHUAI
A1 - Xian-jun DAI
A1 - Wei-huan FANG
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 2
SP - 148
EP - 153
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0710395

porcine circovirus type 2 (PCV2) is closely related to the postweaning multisystemic wasting syndrome (PMWS). In this study, the pig serum and tissue samples collected from different regions of Hangzhou District in Zhejiang Province of China between 2003 and 2005 were analyzed by enzyme-linked immunosorbent assay (ELISA) for PCV2 antibody and by polymerase chain reaction (PCR) for ORF2 gene. The results show that out of 1250 randomly collected serum samples, 500 sera (40%) were seropositive for PCV2. PCR results demonstrate that Hangzhou PCV2 with more than 50% Chinese PCV2 strains and French PCV2 formed Cluster A. Only one PCV2 from Hangzhou belonged to Cluster B with some other Chinese PCV2 and Netherlands’s isolates. Cluster C consisted of PCV2 isolates from China, US, Canada, UK and Germany. The results indicate that the PCV2 infection was widespread in Hangzhou.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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