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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.2 P.132~140


Molecular and functional comparisons of the vacuolar Na+/H+ exchangers originated from glycophytic and halophytic species

Author(s):  Jin-yao LI, Xiao-wei HE, Li XU, Jie ZHOU, Ping WU, Hui-xia SHOU, Fu-chun ZHANG

Affiliation(s):  State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   huixia@zju.edu.cn, zfcxju@xju.edu.cn

Key Words:  NHX gene, Rice transformation, Salt stress, Tolerance

Jin-yao LI, Xiao-wei HE, Li XU, Jie ZHOU, Ping WU, Hui-xia SHOU, Fu-chun ZHANG. Molecular and functional comparisons of the vacuolar Na+/H+ exchangers originated from glycophytic and halophytic species[J]. Journal of Zhejiang University Science B, 2008, 9(2): 132~140.

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author="Jin-yao LI, Xiao-wei HE, Li XU, Jie ZHOU, Ping WU, Hui-xia SHOU, Fu-chun ZHANG",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Molecular and functional comparisons of the vacuolar Na+/H+ exchangers originated from glycophytic and halophytic species
%A Jin-yao LI
%A Xiao-wei HE
%A Li XU
%A Ping WU
%A Hui-xia SHOU
%A Fu-chun ZHANG
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 2
%P 132~140
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0710445

T1 - Molecular and functional comparisons of the vacuolar Na+/H+ exchangers originated from glycophytic and halophytic species
A1 - Jin-yao LI
A1 - Xiao-wei HE
A1 - Li XU
A1 - Jie ZHOU
A1 - Ping WU
A1 - Hui-xia SHOU
A1 - Fu-chun ZHANG
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 2
SP - 132
EP - 140
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0710445

A novel vacuolar Na+/H+ exchanger, CgNHX1, was cloned from a halophytic species Chenopodium glaucum by using reverse transcriptase-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) technique. Sequence alignment and phylogenetic analysis of 22 NHX genes from GenBank as well as the new CgNHX1 gene indicate that NHX genes shared a great degree of similarity, regardless of their glycophytic or halophytic origin. Expression of the CgNHX1 gene was induced by NaCl and peaked at 400 mmol/L NaCl. Overexpression of NHX1 genes in rice enhanced their tolerance to salt stress. However, there is no significant difference in salt tolerance among the transgenic rice plants overexpressing the NHX1 genes from either glycophytic or halophytic species. The Na+ content of both the wild type (WT) and transgenic plants increased when exposed to 50 and 100 mmol/L NaCl, and the Na+ concentration in transgenic plants was marginally higher than that of WT. Our data demonstrate that the overexpression of the NHX1 gene from either glycophytic or halophytic species resulted in the enhanced tolerance to salt stress at a similar level, suggesting that NHX gene per se might not be the reason accounting for the difference in salt tolerance between glycophytes and halophytes.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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