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Received: 2007-08-22

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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.4 P.299~305

http://doi.org/10.1631/jzus.B0710446


Gene transfer into primary cultures of fetal neural stem cells by a recombinant adenovirus carrying the gene for green fluorescent protein


Author(s):  Yong FU, Shen-qing WANG, Ying-peng LIU, Guo-peng WANG, Jian-ting WANG, Shu-sheng GONG

Affiliation(s):  Department of Otorhinolaryngology, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China; more

Corresponding email(s):   fyent@163.com

Key Words:  Recombinant adenovirus vector, Viral infection, Fetal neural stem cells, Green fluorescent protein


Yong FU, Shen-qing WANG, Ying-peng LIU, Guo-peng WANG, Jian-ting WANG, Shu-sheng GONG. Gene transfer into primary cultures of fetal neural stem cells by a recombinant adenovirus carrying the gene for green fluorescent protein[J]. Journal of Zhejiang University Science B, 2008, 9(4): 299~305.

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author="Yong FU, Shen-qing WANG, Ying-peng LIU, Guo-peng WANG, Jian-ting WANG, Shu-sheng GONG",
journal="Journal of Zhejiang University Science B",
volume="9",
number="4",
pages="299~305",
year="2008",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0710446"
}

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%T Gene transfer into primary cultures of fetal neural stem cells by a recombinant adenovirus carrying the gene for green fluorescent protein
%A Yong FU
%A Shen-qing WANG
%A Ying-peng LIU
%A Guo-peng WANG
%A Jian-ting WANG
%A Shu-sheng GONG
%J Journal of Zhejiang University SCIENCE B
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%P 299~305
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0710446

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T1 - Gene transfer into primary cultures of fetal neural stem cells by a recombinant adenovirus carrying the gene for green fluorescent protein
A1 - Yong FU
A1 - Shen-qing WANG
A1 - Ying-peng LIU
A1 - Guo-peng WANG
A1 - Jian-ting WANG
A1 - Shu-sheng GONG
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 4
SP - 299
EP - 305
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0710446


Abstract: 
Objective: To evaluate the transduction efficiency of a recombinant adenovirus carrying the gene for green fluorescent protein (Ad-GFP) into the primary cultures of fetal neural stem cells (NSCs) by the expression of GFP. Methods: The Ad-GFP was constructed by homologous recombination in bacteria with the AdEasy system; NSCs were isolated from rat fetal hippocampus and cultured as neurosphere suspensions. After infection with the recombinant Ad-GFP, NSCs were examined with a fluorescent microscopy and a flow cytometry for their expression of GFP. Results: After the viral infection, flow cytometry analysis revealed that the percentage of GFP-positive cells was as high as 97.05%. The infected NSCs sustained the GFP expression for above 4 weeks. After differentiated into astrocytes or neurons, they continued to express GFP efficiently. Conclusion: We have successfully constructed a viral vector Ad-GFP that can efficiently infect the primary NSCs. The reporter gene was showed fully and sustained expression in the infected cells as well as their differentiated progenies.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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