Full Text:   <1506>

CLC number: TQ9; Q819

On-line Access: 

Received: 2007-07-10

Revision Accepted: 2007-09-30

Crosschecked: 0000-00-00

Cited: 2

Clicked: 4047

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.1 P.77~84

10.1631/jzus.B071372


Medium optimization for enhanced production of cytosine-substituted mildiomycin analogue (MIL-C) by Streptoverticillium rimofaciens ZJU 5119


Author(s):  Dan YE, Zhi-nan XU, Pei-lin CEN

Affiliation(s):  Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China

Corresponding email(s):   znxu@zju.edu.cn

Key Words:  Cytosine-substituted mildiomycin analogue (MIL-C), Plackett-Burman design, Response surface methodology, Streptoverticillium rimofaciens, Nucleoside antibiotic


Share this article to: More <<< Previous Article|

Dan YE, Zhi-nan XU, Pei-lin CEN. Medium optimization for enhanced production of cytosine-substituted mildiomycin analogue (MIL-C) by Streptoverticillium rimofaciens ZJU 5119[J]. Journal of Zhejiang University Science B, 2008, 9(1): 77~84.

@article{title="Medium optimization for enhanced production of cytosine-substituted mildiomycin analogue (MIL-C) by Streptoverticillium rimofaciens ZJU 5119",
author="Dan YE, Zhi-nan XU, Pei-lin CEN",
journal="Journal of Zhejiang University Science B",
volume="9",
number="1",
pages="77~84",
year="2008",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B071372"
}

%0 Journal Article
%T Medium optimization for enhanced production of cytosine-substituted mildiomycin analogue (MIL-C) by Streptoverticillium rimofaciens ZJU 5119
%A Dan YE
%A Zhi-nan XU
%A Pei-lin CEN
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 1
%P 77~84
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B071372

TY - JOUR
T1 - Medium optimization for enhanced production of cytosine-substituted mildiomycin analogue (MIL-C) by Streptoverticillium rimofaciens ZJU 5119
A1 - Dan YE
A1 - Zhi-nan XU
A1 - Pei-lin CEN
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 1
SP - 77
EP - 84
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B071372


Abstract: 
cytosine-substituted mildiomycin analogue (MIL-C) was produced effectively by supplementing cytosine into the culture of Streptoverticillium rimofaciens. In order to improve the yield of MIL-C, statistically-based experimental designs were applied to optimize the fermentation medium for S. rimofaciens ZJU 5119. Fifteen culture conditions were examined for their significances on MIL-C production using plackett-Burman design. The plackett-Burman design and one-variable-at-a-time design indicated that glucose and rice meal as the complex carbon sources, and peanut cake meal and NH4NO3 as the complex nitrogen sources were beneficial for MIL-C production in S. rimofaciens ZJU 5119. The results of further central composition design (CCD) showed that the optimal concentration of glucose, rice meal and peanut cake meal were 18.7 g/L, 64.8 g/L and 65.1 g/L, respectively. By using this optimal fermentation medium, the MIL-C concentration was increased up to 1336.5 mg/L, an approximate 3.8-fold improvement over the previous concentration (350.0 mg/L) with un-optimized medium. This work will be very helpful to the large-scale production of MIL-C in the future.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1] Chen, Y., Qu, H.B., Xu, Z.N., Cheng, Y.Y., 2006. Ion-exchange properties of mildiomycin on HZ110 (TM) resin. Korean J. Chem. Eng., 23(6):991-996.

[2] Choi, D.B., Park, E.Y., Okabe, M., 2000. Dependence of apparent viscosity on mycelial morphology of Streptomyces fradiae culture in various nitrogen sources. Biotechnol. Prog., 16(4):525-532.

[3] Didier, D., Etcheverrigaray, M., Kratje, R., Goicoechea, H.C., 2007. Crossed mixture design and multiple response analysis for developing complex culture media used in recombinant protein production. Chemometrics and Intelligent Laboratory Systems, 86(1):1-9.

[4] Farid, M.A., El-Enshasy, H.A., El-Diwany, A.I., El-Sayed, E.S.A., 2000. Optimization of the cultivation medium for natamycin production by Streptomyces natalensis. J. Basic Microbiol., 40(3):157-166.

[5] Harada, S., Mizuta, E., Kishi, T., 1978. Structure of mildiomycin, a new antifungal nucleoside antibiotic. J. Am. Chem. Soc., 100(15):4895-4897.

[6] Iwasa, T., Suetomi, K., Kusaka, T., 1978. Taxonomic and fermentation of producing organism and antimicrobial activity of mildiomycin. J. Antibiot., 31(6):511-518.

[7] Kishimoto, K., Park, Y.S., Okabe, M., Akiyama, S., 1997. Effect of ferrous ion on amino acid metabolism in mildiomycin production by Streptoverticillium rimofaciens. J. Antibiot., 50(3):206-211.

[8] Kumar, P., Satyanarayana, T., 2007. Optimization of culture variables for improving glucoamylase production by alginate-entrapped Thermomucor indicae-seudaticae using statistical methods. Bioresour. Technol., 98(6):1252-1259.

[9] Liang, L.F., Ye, D., Xu, Z.N., Cen, P.L., 2006. Biosynthesis of a mildiomycin derivative and its structure identification. J. Zhejiang Univ. (Eng. Sci.), 40(11):1909-1913 (in Chinese).

[10] Maddox, I.S., Richert, S.H., 1977. Use of response surface methodology for the rapid optimization of microbiological media. J. Appl. Bacteriol., 43(2):197-204.

[11] Mao, X.Z., Shen, Y.L., Yang, L., Chen, S., Yang, Y.P., Yang, J.Y., Zhu, H., Deng, Z.X., Wei, D.Z., 2007. Optimizing the medium compositions for accumulation of the novel FR-008/candicidin derivatives CS101 by a mutant of Streptomyces sp. using statistical experimental methods. Process Biochem., 42(5):878-883.

[12] Peter, C.P., Suzuki, Y., Buchs, J., 2006. Hydromechanical stress in shake flasks: correlation for the maximum local energy dissipation rate. Biotech. Bioeng., 93(6):1165-1176.

[13] Prakash, G., Srivastava, A.K., 2005. Statistical media optimization for cell growth and azadirachtin production in Azadirachta indica (A. Juss) suspension cultures. Process Biochem., 40(12):3795-3800.

[14] Sharma, D.C., Satyanarayana, T., 2006. A marked enhancement in the production of a highly alkaline and thermostable pectinase by Bacillus pumilus dcsr1 in submerged fermentation by using statistical methods. Bioresour. Technol., 97(5):727-733.

[15] Shi, F., Xu, Z.N., Cen, P.L., 2006. Optimization of γ-polyglutamic acid production by Bacillus subtilis ZJU-7 using a surface-response methodology. Biotechnol. Bioprocess Eng., 11(3):251-257.

[16] Suzuki, T., Sawada, H., Asai, T., 1982. Method for Producing Mildiomycin. US Patent, No. 4334022.

[17] Takatsuki, T.S., Neyagawa, H.S., Suita, K.K., 1981. Mildiomycin Analogs and a Method of Production. US Patent No. 4296107.

[18] Xie, Z.P., Xu, Z.N., Shen, W.H., Cen, P.L., 2005. Bioassay of mildiomycin and a rapid, cost-effective agar plug method for screening high-yielding mutants of mildiomycin. World J. Microbiol. Biotechnol., 21(8-9):1433-1437.

[19] Ye, D., Shen, W.H., Liang, L.F., Xu, Z.N., Cen, P.L., 2006. Advances on bio-pesticide mildiomycin and its analogs. Pesticide, 45(12):799-804 (in Chinese).

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE