CLC number: Q819
On-line Access:
Received: 2007-09-07
Revision Accepted: 2007-12-04
Crosschecked: 0000-00-00
Cited: 6
Clicked: 6924
You LIU, Qun WANG, Ying-bin PAN, Zhi-jie GAO, Yan-fen LIU, Shao-hong CHEN. Effects of over-expressing resistin on glucose and lipid metabolism in mice[J]. Journal of Zhejiang University Science B, 2008, 9(1): 44-50.
@article{title="Effects of over-expressing resistin on glucose and lipid metabolism in mice",
author="You LIU, Qun WANG, Ying-bin PAN, Zhi-jie GAO, Yan-fen LIU, Shao-hong CHEN",
journal="Journal of Zhejiang University Science B",
volume="9",
number="1",
pages="44-50",
year="2008",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B071479"
}
%0 Journal Article
%T Effects of over-expressing resistin on glucose and lipid metabolism in mice
%A You LIU
%A Qun WANG
%A Ying-bin PAN
%A Zhi-jie GAO
%A Yan-fen LIU
%A Shao-hong CHEN
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 1
%P 44-50
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B071479
TY - JOUR
T1 - Effects of over-expressing resistin on glucose and lipid metabolism in mice
A1 - You LIU
A1 - Qun WANG
A1 - Ying-bin PAN
A1 - Zhi-jie GAO
A1 - Yan-fen LIU
A1 - Shao-hong CHEN
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 1
SP - 44
EP - 50
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B071479
Abstract: resistin, a newly discovered peptide hormone mainly secreted by adipose tissues, is present at high levels in serum of obese mice and may be a potential link between obesity and insulin resistance in rodents. However, some studies of rat and mouse models have associated insulin resistance and obesity with decreased resistin expression. In humans, no relationship between resistin level and insulin resistance or adiposity was observed. This suggests that additional studies are necessary to determine the specific role of resistin in the regulation of energy metabolism and adipogenesis. In the present study, we investigated the effect of resistin in vivo on glucose and lipid metabolism by over-expressing resistin in mice by intramuscular injection of a recombinant eukaryotic expression vector pcDNA3.1-Retn encoding porcine resistin gene. After injection, serum resistin and serum glucose (GLU) levels were significantly increased in the pcDNA3.1-Retn-treated mice; there was an obvious difference in total cholesterol (TC) level between the experiment and the control groups on Day 30. In pcDNA3.1-Retn-treated mice, both free fatty acid (FFA) and high density lipoprotein (HDL) cholesterol levels were markedly lower than those of control, whereas HDL cholesterol and triglyceride (TG) levels did not differ between the two groups. Furthermore, lipase activity was expressly lower on Day 20. Our data suggest that resistin over-expressed in mice might be responsible for insulin resistance and parameters related to glucose and lipid metabolism were changed accordingly.
[1] Banerjee, R.R., Rangwala, S.M., Shapiro, J.S., Rich, A.S., Rhoades, B., Qi, Y., Wang, J., Rajala, M.W., Pocai, A., Scherer, P.E., et al., 2004. Regulation of fasted blood glucose by resistin. Science, 303(5661):1195-1198.
[2] Bastard, J.P., Maachi, M., Lagathu, C., Kim, M.J., Caron, M., Vidal, H., Capeau, J., Feve, B., 2006. Recent advance in the relationship between obesity, inflammation, and insulin resistance. Eur. Cytokine Netw., 17(1):4-12.
[3] Borst, S.E., Lee, Y., Conover, C.F., Shek, E.W., Bagby, G.J., 2004. Neutralization of tumor necrosis factor-alpha reverses insulin resistance in skeletal muscle but not adipose tissue. Am. J. Physiol. Endocrinol. Metab., 287(5):E934-938.
[4] Chen, L., Nyomba, B.L., 2003. Glucose intolerance and resistin expression in rat offspring exposed to ethanol in utero: modulation by postnatal high-fat diet. Endocrinology, 144(2):500-508.
[5] Dai, M.H., Xia, T., Chen, X.D., Gan, L., Feng, S.Q., Qiu, H., Peng, Y., Yang, Z.Q., 2006. Cloning and characterization of porcine resistin gene. Domest. Anim. Endocrino., 30(2):88-97.
[6] Dixon, J.L., Ginsberg, H.N., 1993. Regulation of hepatic secretion of apolipoprotein B-containing lipoproteins: information obtained from cultured liver cells. J. Lipid. Res., 34:167-179.
[7] Fain, J.N., Cheema, P.S., Bahouth, S.W., Hiler, M., 2003. Resistin release by human adipose tissues explants in primary culture. Biochem. Biophys. Res. Commun., 300(3):674-678.
[8] Friedman, J.M., 2000. Obesity in the new millennium. Nature, 404(6778):632-634.
[9] Gao, Z., Zhang, X., Zuberi, A., Hwang, D., Quon, M.J., Lefevre, M., Ye, J., 2004. Inhibition of insulin sensitivity by free fatty acids requires activation of multiple serine kinases in 3T3-L1 adipocytes. Mol. Endocrinol., 18(8):2024-2034.
[10] Gong, H., Ni, Y., Guo, X., Fei, L., Pan, X., Guo, M., Chen, R., 2004. Resistin promotes 3T3-L1 preadipocyte differentiation. Eur. J. Endocrinol., 150(6):885-892.
[11] Hegarty, B.D., Cooney, G.J., Kraegen, E.W., Furler, S.M., 2002. Increased efficiency of fatty acid uptake contributes to lipid accumulation in skeletal muscle of high fat-fed insulin-resistant rats. Diabetes, 51(5):1477-1484.
[12] Kim, K.H., Lee, K., Moon, Y.S., 2001. A cysteine-rich adipose tissue-specific secretory factor inhibits adipocyte differentiation. J. Biol. Chem., 276(14):11252-11256.
[13] Kitagawa, Y., Bujo, H., Takahashi, K., Shibasaki, M., Ishikawa, K., Yagui, K., Hashimoto, N., Noda, K., Nakamura, T., Yano, S., et al., 2004. Impaired glucose tolerance is accompanied by decreased insulin sensitivity in tissues of mice implanted with cells that overexpress resistin. Diabetologia, 47(10):1847-1853.
[14] Lewis, G.F., 1999. Fatty acid regulation of very low density lipoprotein (VLDL) production. Curr. Opin. Lipidol., 10(5):475-477.
[15] Niederwanger, A., Kranebitter, M., Ciardi, C., Tatarczyk, T., Patsch, J.R., Pedrini, M.T., 2007. Resistin impairs basaland insulin-induced glycogen synthesis by different mechanisms. Mol. Cell. Endocrinol., 263(1-2):112-119.
[16] Palanivel, R., Sweeney, G., 2005. Regulation of fatty acid uptake and metabolism in L6 skeletal muscle cells by resistin. FEBS Lett., 579(22):5049-5054.
[17] Palanivel, R., Maida, A., Liu, Y., Sweeney, G., 2006. Regulation of insulin signalling, glucose uptake and metabolism in rat skeletal muscle cells upon prolonged exposure to resistin. Diabetologia, 49(1):183-190.
[18] Pravenec, M., Kazdova, L., Landa, V., Zidek, V., Mlejnek, P., Jansa, P., Wang, J., Qi, N., Kurtz, T.W., 2003. Transgenic and recombinant resistin impair skeletal muscle glucose metabolism in the spontaneously hypertensive rat. J. Biol. Chem., 278(46):45209-45215.
[19] Rajala, M.W., Obici, S., Scherer, P.E., Rossetti, L., 2003. Adipose-derived resistin and gut-derived resistin-like molecule-beta selectively impair insulin action on glucose production. J. Clin. Invest., 111(2):225-230.
[20] Sato, N., Kobayashi, K., Inoguchi, T., Sonoda, N., Imamura, M., Sekiguchi, N., Nakashima, N., Nawata, H., 2005. Adenovirus-mediated high expression of resistin causes dyslipidemia in mice. Endocrinology, 146(1):273-279.
[21] Satoh, H., Nguyen, M.T., Miles, P.D., Imamura, T., Usui, I., Olefsky, J.M., 2004. Adenovirus-mediated chronic “hyper-resistinemia” leads to in vivo insulin resistance in normal rats. J. Clin. Invest., 114(2):224-231.
[22] Savage, D.B., Sewter, C.P., Klenk, E.S., Segal, D.G., Vidal-Puig, A., Considine, R.V., O'Rahilly, S., 2001. Resistin/Fizz3 expression in relation to obesity and peroxisome proliferator-activated receptor-gamma action in humans. Diabetes, 50(10):2199-2202.
[23] Steppan, C.M., Lazar, M.A., 2002. Resistin and obesity-associated insulin resistance. Trends Endocrinol. Metab., 13(1):18-23.
[24] Steppan, C.M., Bailey, S.T., Bhat, S., Brown, E.J., Banerjee, R.R., Wright, C.M., Patel, H.R., Ahima, R.S., Lazar, M.A., 2001a. The hormone resistin links obesity to diabetes. Nature, 409(6818):307-312.
[25] Steppan, C.M., Brown, E.J., Wright, C.M., Bhat, S., Banerjee, R.R., Dai, C.Y., Enders, G.H., Silberg, D.G., Wen, X., Wu, G.D., et al., 2001b. A family of tissue-specific resistin-like molecules. Proc. Natl. Acad. Sci. USA, 98(2):502-506.
[26] Steppan, C.M., Wang, J., Whiteman, E.L., Birnbaum, M.J., Lazar, M.A., 2005. Activation of SOCS-3 by resistin. Mol. Cell. Biol., 25(4):1569-1575.
[27] Yamauchi, T., Kamon, J., Ito, Y., Tsuchida, A., Yokomizo, T., Kita, S., Sugiyama, T., Miyagishi, M., Hara, K., Tsunoda, M., et al., 2003. Cloning of adiponectin receptors that mediate antidiabetic metabolic effects. Nature, 423(6941):762-769.
Open peer comments: Debate/Discuss/Question/Opinion
<1>