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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.6 P.455~463


T-2 toxin-induced apoptosis involving Fas, p53, Bcl-xL, Bcl-2, Bax and caspase-3 signaling pathways in human chondrocytes

Author(s):  Jing-hong CHEN, Jun-ling CAO, Yong-lie CHU, Zhi-lun WANG, Zhan-tian YANG, Hong-lin WANG

Affiliation(s):  Ministry of Education Key Laboratory of Environment and Genes related to Diseases, Institute of Endemic Diseases, School of Medicine, Xi’ more

Corresponding email(s):   Jixiang46@163.com, wzlun@mail.xjtu.edu.cn

Key Words:  Apoptosis, Apoptosis-related proteins, Chondrocyte, T-2 toxin

Jing-hong CHEN, Jun-ling CAO, Yong-lie CHU, Zhi-lun WANG, Zhan-tian YANG, Hong-lin WANG. T-2 toxin-induced apoptosis involving Fas, p53, Bcl-xL, Bcl-2, Bax and caspase-3 signaling pathways in human chondrocytes[J]. Journal of Zhejiang University Science B, 2008, 9(6): 455~463.

@article{title="T-2 toxin-induced apoptosis involving Fas, p53, Bcl-xL, Bcl-2, Bax and caspase-3 signaling pathways in human chondrocytes",
author="Jing-hong CHEN, Jun-ling CAO, Yong-lie CHU, Zhi-lun WANG, Zhan-tian YANG, Hong-lin WANG",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T T-2 toxin-induced apoptosis involving Fas, p53, Bcl-xL, Bcl-2, Bax and caspase-3 signaling pathways in human chondrocytes
%A Jing-hong CHEN
%A Jun-ling CAO
%A Yong-lie CHU
%A Zhi-lun WANG
%A Zhan-tian YANG
%A Hong-lin WANG
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 6
%P 455~463
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820013

T1 - T-2 toxin-induced apoptosis involving Fas, p53, Bcl-xL, Bcl-2, Bax and caspase-3 signaling pathways in human chondrocytes
A1 - Jing-hong CHEN
A1 - Jun-ling CAO
A1 - Yong-lie CHU
A1 - Zhi-lun WANG
A1 - Zhan-tian YANG
A1 - Hong-lin WANG
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 6
SP - 455
EP - 463
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820013

Objective: To investigate the effects of t-2 toxin on expressions of Fas, p53, Bcl-xL, Bcl-2, Bax and caspase-3 on human chondrocytes. Methods: Human chondrocytes were treated with t-2 toxin (1~20 ng/ml) for 5 d. Fas, p53 and other apoptosis-related proteins such as Bax, Bcl-2, Bcl-xL, caspase-3 were determined by Western blot analysis and their mRNA expressions were determined by reverse transcriptase-polymerase chain reaction (RT-PCR). Results: Increases in Fas, p53 and the pro-apoptotic factor Bax protein and mRNA expressions and a decrease of the anti-apoptotic factor Bcl-xL were observed in a dose-dependent manner after exposures to 1~20 ng/ml t-2 toxin, while the expression of the anti-apoptotic factor Bcl-2 was unchanged. Meanwhile, t-2 toxin could also up-regulate the expressions of both pro-caspase-3 and caspase-3 in a dose-dependent manner. Conclusion: These data suggest a possible underlying molecular mechanism for t-2 toxin to induce the apoptosis signaling pathway in human chondrocytes by regulation of apoptosis-related proteins.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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