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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.7 P.511~519

http://doi.org/10.1631/jzus.B0820015


Magnaporthe oryzae MTP1 gene encodes a type III transmembrane protein involved in conidiation and conidial germination


Author(s):  Qin LU, Jian-ping LU, Xiao-dong LI, Xiao-hong LIU, Hang MIN, Fu-cheng LIN

Affiliation(s):  College of Life Sciences, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   jplu@zju.edu.cn, fuchenglin@zju.edu.cn

Key Words:  Magnaporthe oryzae, Magnaporthe grisea, MTP1, cDNA, Gene knockout, Conidial germination


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Qin LU, Jian-ping LU, Xiao-dong LI, Xiao-hong LIU, Hang MIN, Fu-cheng LIN. Magnaporthe oryzae MTP1 gene encodes a type III transmembrane protein involved in conidiation and conidial germination[J]. Journal of Zhejiang University Science B, 2008, 9(7): 511~519.

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author="Qin LU, Jian-ping LU, Xiao-dong LI, Xiao-hong LIU, Hang MIN, Fu-cheng LIN",
journal="Journal of Zhejiang University Science B",
volume="9",
number="7",
pages="511~519",
year="2008",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0820015"
}

%0 Journal Article
%T Magnaporthe oryzae MTP1 gene encodes a type III transmembrane protein involved in conidiation and conidial germination
%A Qin LU
%A Jian-ping LU
%A Xiao-dong LI
%A Xiao-hong LIU
%A Hang MIN
%A Fu-cheng LIN
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 7
%P 511~519
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820015

TY - JOUR
T1 - Magnaporthe oryzae MTP1 gene encodes a type III transmembrane protein involved in conidiation and conidial germination
A1 - Qin LU
A1 - Jian-ping LU
A1 - Xiao-dong LI
A1 - Xiao-hong LIU
A1 - Hang MIN
A1 - Fu-cheng LIN
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 7
SP - 511
EP - 519
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820015


Abstract: 
In this study the MTP1 gene, encoding a type III integral transmembrane protein, was isolated from the rice blast fungus Magnaporthe oryzae. The Mtp1 protein is 520 amino acids long and is comparable to the Ytp1 protein of Saccharomyces cerevisiae with 46% sequence similarity. Prediction programs and MTP1-GFP (green fluorescent protein) fusion expression results indicate that Mtp1 is a protein located at several membranes in the cytoplasm. The functions of the MTP1 gene in the growth and development of the fungus were studied using an MTP1 gene knockout mutant. The MTP1 gene was primarily expressed at the hyphal and conidial stages and is necessary for conidiation and conidial germination, but is not required for pathogenicity. The ΔMTP1 mutant grew more efficiently than the wild type strain on non-fermentable carbon sources, implying that the MTP1 gene has a unique role in respiratory growth and carbon source use.

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