CLC number: R72
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
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Hai-ju ZHANG, Ruo-peng SUN, Ge-fei LEI, Lu YANG, Chun-xi LIU. Cyclooxygenase-2 inhibitor inhibits hippocampal synaptic reorganization in pilocarpine-induced status epilepticus rats[J]. Journal of Zhejiang University Science B, 2008, 9(11): 903-915.
@article{title="Cyclooxygenase-2 inhibitor inhibits hippocampal synaptic reorganization in pilocarpine-induced status epilepticus rats",
author="Hai-ju ZHANG, Ruo-peng SUN, Ge-fei LEI, Lu YANG, Chun-xi LIU",
journal="Journal of Zhejiang University Science B",
volume="9",
number="11",
pages="903-915",
year="2008",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0820018"
}
%0 Journal Article
%T Cyclooxygenase-2 inhibitor inhibits hippocampal synaptic reorganization in pilocarpine-induced status epilepticus rats
%A Hai-ju ZHANG
%A Ruo-peng SUN
%A Ge-fei LEI
%A Lu YANG
%A Chun-xi LIU
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 11
%P 903-915
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820018
TY - JOUR
T1 - Cyclooxygenase-2 inhibitor inhibits hippocampal synaptic reorganization in pilocarpine-induced status epilepticus rats
A1 - Hai-ju ZHANG
A1 - Ruo-peng SUN
A1 - Ge-fei LEI
A1 - Lu YANG
A1 - Chun-xi LIU
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 11
SP - 903
EP - 915
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820018
Abstract: Objective: To examine modulations caused by cyclooxygenase-2 (COX-2) inhibitors on altered microenvironments and overbalanced neurotransmitters in pilocarpine-induced epileptic status rats and to investigate possible mechanisms. Methods: Celecoxib (a COX-2 inhibitor) was administered 45 min prior to pilocarpine administration. The effects of COX-2 inhibitors on mIPSCs (miniature GABAergic inhibitory postsynaptic currents) of CA3 pyramidal cells in the hippocampus were recorded. Expressions of COX-2, c-Fos, newly generated neurons, and activated microgliosis were analyzed by immunohistochemistry, and expressions of α-subunit of γ-amino butyric acid (GABAA) receptors and mitogen-activated protein kinase/extracellular signal-regulated protein kinase (MAPK/ERK) activity were detected by Western blotting. Results: Pretreatment with celecoxib showed protection against pilocarpine-induced seizures. Celecoxib prevented microglia activation in the hilus and inhibited the abnormal neurogenesis and astrogliosis in the hippocampus by inhibiting MAPK/ERK activity and c-Fos transcription. Celecoxib also up-regulated the expression of GABAA receptors. NS-398 (N-2-cyclohexyloxy-4-nitrophenyl-methanesulfonamide), another COX-2 inhibitor, enhanced the frequency and decay time of mIPSCs. Conclusion: The COX-2 inhibitor celecoxib decreased neuronal excitability and prevented epileptogenesis in pilocarpine-induced status epilepticus rats. Celecoxib regulates synaptic reorganization by inhibiting astrogliosis and ectopic neurogenesis by attenuating MAPK/ERK signal activity, mediated by a GABAergic mechanism.
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