Full Text:   <2289>

CLC number: Q53

On-line Access: 

Received: 2008-01-20

Revision Accepted: 2008-03-08

Crosschecked: 0000-00-00

Cited: 32

Clicked: 4072

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.9 P.721~727

10.1631/jzus.B0820025


Preliminary studies on the chemical characterization and antioxidant properties of acidic polysaccharides from Sargassum fusiforme


Author(s):  Jing ZHOU, Nan HU, Ya-lin WU, Yuan-jiang PAN, Cui-rong SUN

Affiliation(s):  Department of Chemistry, Zhejiang University, Hangzhou 310027, China

Corresponding email(s):   suncuirong@zju.edu.cn

Key Words:  Sargassum fusiforme, Polysaccharide, Antioxidant activity, Uronic acid, Molecular weight


Jing ZHOU, Nan HU, Ya-lin WU, Yuan-jiang PAN, Cui-rong SUN. Preliminary studies on the chemical characterization and antioxidant properties of acidic polysaccharides from Sargassum fusiforme[J]. Journal of Zhejiang University Science B, 2008, 9(9): 721~727.

@article{title="Preliminary studies on the chemical characterization and antioxidant properties of acidic polysaccharides from Sargassum fusiforme",
author="Jing ZHOU, Nan HU, Ya-lin WU, Yuan-jiang PAN, Cui-rong SUN",
journal="Journal of Zhejiang University Science B",
volume="9",
number="9",
pages="721~727",
year="2008",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0820025"
}

%0 Journal Article
%T Preliminary studies on the chemical characterization and antioxidant properties of acidic polysaccharides from Sargassum fusiforme
%A Jing ZHOU
%A Nan HU
%A Ya-lin WU
%A Yuan-jiang PAN
%A Cui-rong SUN
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 9
%P 721~727
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820025

TY - JOUR
T1 - Preliminary studies on the chemical characterization and antioxidant properties of acidic polysaccharides from Sargassum fusiforme
A1 - Jing ZHOU
A1 - Nan HU
A1 - Ya-lin WU
A1 - Yuan-jiang PAN
A1 - Cui-rong SUN
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 9
SP - 721
EP - 727
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820025


Abstract: 
In order to investigate the antioxidant properties of the polysaccharides from the brown alga Sargassum fusiforme, the crude polysaccharides from S. fusiforme (SFPS) were extracted in hot water, and the lipid peroxidation inhibition assay exhibited that SFPS possessed a potential antioxidant activity. Hence, two purely polymeric fractions, SFPS-1 and SFPS-2 were isolated by the column of DEAE (2-diethylaminoethanol)-Sepharose Fast Flow, with their molecular weights of 51.4 and 30.3 kDa determined by high performance gel permeation chromatography (HPGPC). They were preliminarily characterized using chemical analysis in combination of infrared (IR) and nuclear magnetic resonance (NMR) spectroscopies and found to contain large amounts of uronic acids and β-glycosidical linkages. The antioxidant activities of these two SFPS fractions were evaluated using superoxide and hydroxyl radical-scavenging assays. The results show that the antioxidant ability of SFPS-2 was higher than that of SFPS-1, probably correlating with the molecular weight and uronic acid content.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1] Bae, S.W., Suh, H.J., 2007. Antioxidant activities of five different mulberry cultivars in Korea. LWT-Food Science and Technology, 40(6):955-962.

[2] Barker, S.A., Bourne, E.J., Stacey, M., Whiffen, D.H., 1954. Infra-redspectra of carbohydrates. Part I. Some derivatives of D-glucopyranose. J. Chem. Soc., 1954:171-176.

[3] Bitter, T., Muir, H.M., 1962. A modified uronic acid carbazole reaction. Anal. Biochem., 4(4):330-334.

[4] Butterfield, D.A., Castenga, A., Pocernich, C.B., Drake, J., Scapagnini, G., Calabrese, V., 2002. Nutritional approaches to combat oxidative stress in Alzheimer’s diseases. J. Nutr. Biochem., 13(8):444-461.

[5] Chen, H., Zhang, M., Xie, B., 2004. Quantification of uronic acids in tea polysaccharide conjugates and their antioxidant properties. J. Agric. Food Chem., 52(11):3333-3336.

[6] Chiarini, L., Cescutti, P., Drigo, L., Impallomeni, G., Herasimenka, Y., Bevivino, A., Dalmastri, C., Tabacchioni, S., Manno, G., Zanetti, F., Rizzo, R., 2004. Exopolysaccharides produced by Burkholderia cenocepacia recA lineages IIIA and IIIB. J. Cystic Fibr., 3(3):165-172.

[7] Cozzolino, R., Malvagna, P., Spina, E., Giori, A., Fuzzati, N., Anelli, A., Garozzo, D., Impallomeni, G., 2006. Structural analysis of the polysaccharides from Echinacea angustifolia radix. Carbohydr. Polym., 65(3):263-272.

[8] Crowell, E.P., Burnett, B.B., 1967. Determination of the carbohydrate composition of wood pulps by gas chromatography of the alditol acetates. Anal. Chem., 39(1):121-124.

[9] Dawczynski, C., Schubert, R., Jahreis, G., 2007. Amino acids, fatty acids, and dietary fibre in edible seaweed products. Food Chem., 103(3):891-899.

[10] Diplock, A.T., Charleux, J.L., Crozier-Willi, G., Kok, F.J., Rice-Evans, C., Robefroid, M., 1998. Functional food science and defense against reactive oxidative species. Br. J. Nutr., 80(1):S77-S112.

[11] Duan, X.J., Zhang, W.W., Li, X.M., Wang, B.G., 2006. Evaluation of antioxidant property of extract and fractions obtained from a red alga, Polysiphonia urceolata. Food Chem., 95(1):37-43.

[12] Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F., 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem., 28(3):350-356.

[13] Halliwell, B., Aruoma, O.I., 1991. DNA damage by oxygen derived species. Its mechanism and measurement in mammalian systems. FEBS Lett., 281(1-2):9-19.

[14] Halliwell, B., Gutteridge, J.M.C., Arurma, O.I., 1987. The deoxyribose method: a simple “test-tube” assay for determination of rate constants for reactions of hydroxyl radicals. Anal. Biochem., 165(1):215-219.

[15] Haroun-Bouhedja, F., Ellouali, M., Sinquin, C., Boisson-Vidal, C., 2000. Relationship between sulfate group and biological activities of fucans. Thromb. Res., 100(5):453-459.

[16] He, S., Lu, Y.B., Wu, B., Pan, Y.J., 2007. Isolation and purification of antioxidative isomeric polyphenols from the roots of Parthenocissus laetevirens by ounter-current chromatograph. J. Chromatogr. A, 1151(1-2):175-179.

[17] Huang, X.X., Zhou, H.Q., Zhang, H., 2006. The effect of Sargassum fusiforme polysaccharide extracts on vibriosis resistance and immune activity of the shrimp. Fenneropenaeus chinensis. Fish Shellfish Immun., 20(5):750-757.

[18] Im, S.A., Oh, S.T., Song, S., Kim, M.R., Kim, D.S., Woo, S.S., Jo, J.H., Park, Y.I., Lee, C.K., 2005. Identification of optimal molecular size of modified Aloe polysaccharides with maximum immunomodulatory activity. Int. Immunopharmacol., 5(2):271-279.

[19] Ji, Y.B., Wang, C., Wu, T., Ji, C.F., 2007. Effect of Sargassum fusiforme polysaccharides on the complex mobility of erythrocytes in tumor-bearing organisms using high performance capillary electrophoresis. Chin. J. Chromatogr., 25(3):322-325.

[20] Juntachote, T., Berghofer, E., 2005. Antioxidative properties and stability of ethanolic extracts of Holy basil and Galangal. Food Chem., 92(2):193-202.

[21] Kuda, T., Tsunekawa, M., Goto, H., Araki, Y., 2005. Antioxidant properties of four edible algae harvested in the Noto Peninsula, Japan. J. Food Compos. Anal., 18(7):625-633.

[22] Lee, J.C., Kim, H.R., Kim, J., Jang, Y.S., 2002. Antioxidant property of an ethanol extract of the stem of Opuntia ficus-indica var. saboten. J. Agric. Food Chem., 50(22):6490-6496.

[23] Leung, M.Y.K., Liu, C., Koon, J.C.M., Fung, K.P., 2006. Polysaccharide biological response modifiers. Immunol. Lett., 105(2):101-114.

[24] Liu, F., Ooi, V.E., Chang, S.T., 1997. Free radical scavenging activities of mushroom polysaccharide extracts. Life Sci., 60(10):763-771.

[25] Mao, W.J., Li, B.F., Gu, Q.Q., Fang, Y.C., Xing, H.T., 2004. Preliminary studies on the chemical characterization and antihyperlipidemic activity of polysaccharide from the brown alga Sargassum fusiforme. Hydrobiologia, 512(1-3):263-266.

[26] Park, P.J., Shahidi, F., Jeon, Y.J., 2004. Antioxidant activities of enzymatic extracts from and edible seaweed Sargassum horneri using ESR spectroscopy. J. Food Lipids, 11(1):15-27.

[27] Peterszegi, G., Robert, A.M., Robert, L., 2003. Protection by L-fucose and fucose-rich polysaccharides against ROS-produced cell death in presence of ascorbate. Biomed. Pharmacother., 57(3-4):130-133.

[28] Rollet-Labelle, E., Grange, M.J., Elbim, C., Marquetty, C., Gougerot-Pocidalo, M.A., Pasquier, C., 1998. Hydroxyl radical as a potential intracellular mediator of polymorphonuclear neutrophil apoptosis. Free Radic. Biol. Med., 24(4):563-572.

[29] Simas, F.F., Maurer-Menestrina, J., Reis, R.A., Sassaki, G.L., Iacomini, M., Gorin, P.A.G., 2006. Structure of the fucose-containing acidic heteroxylan from the gum exudate of Syagrus romanzoffiana (Queen palm). Carbohydr. Polym., 63(1):30-39.

[30] Singthong, J., Cui, S.W., Ningsanond, S., Goff, H.D., 2004. Structural characterization, degree of esterification and some gelling properties of Krueo Ma Noy (Cissampelos pareira) pectin. Carbohydr. Polym., 58(4):391-400.

[31] Tseng, Y.H., Yang, J.H., Mau, J.L., 2008. Antioxidant properties of polysaccharides from Ganoderma tsugae. Food Chem., 107(2):732-738.

[32] Wang, Y., Zhang, M., Ruan, D., Shashkov, A.S., Kilcoyne, M., Savage, A.V., Zhang, L., 2004. Chemical components and molecular mass of six polysaccharides isolated from the sclerotium of Poria cocos. Carbohydr. Res., 339(2):327-334.

[33] Wu, X.M., Ouyang, J.M., Deng, S.P., Cen, Y.Z., 2006. Inhibition of the crystal growth and aggregation of calcium oxalate by algae sulfated polysaccharide in-vitro. Chinese Chem. Lett., 17(1):97-100.

[34] Wu, Y., Hu, N., Pan, Y., Zhou, L., Zhou, X., 2007. Isolation and characterization of a mannoglucan from edible Cordyceps sinensis mycelium. Carbohydrate Res., 342(6):870-875.

[35] Yan, X., Nagata, T., Fan, X., 1998. Antioxidative activities in some common seaweeds. Plant Food Hum. Nutr., 52(3):253-262.

[36] Zhang, L., Lin, Y., 2008. Tannins from Canarium album with potent antioxidant activity. J. Zhejiang Univ. Sci. B, 9(5):407-415.

[37] Zhang, M., Cui, S.W., Cheung, P.C.K., Wang, Q., 2007. Antitumor polysaccharides from mushrooms: a review on their isolation process, structural characteristics and antitumor activity. Trends Food Sci. Tech., 18(1):4-19.

[38] Zhang, Q.B., Li, N., Zhou, G.F., Lu, X.L., Xu, Z.H., Li, Z., 2003. In vivo antioxidant activity of polysaccharide fraction from Porphyra haitanesis (Rhodephyta) in aging mice. Pharmacol. Res., 48(2):151-155.

[39] Zhao, T.T., Zhang, Q.B., Qi, H.M., Zhang, H., Niu, X.Z., Xu, Z.H., Li, Z., 2006. Degradation of porphyran from Porphyra haitanensis and the antioxidant activities of the degraded porphyrans with different molecular weight. Int. J. Biol. Macromol., 38(1):45-50.

[40] Zhu, W., Ooi, V.E.C., Chan, P.K.S., Ang, P.O.Jr., 2003. Isolation and characterization of a sulfated polysaccharide from the brown alga Sargassum patens and determination of its anti-herpes activity. Biochem. Cell Biol., 81(1):25-33.

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE