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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.5 P.385~390

http://doi.org/10.1631/jzus.B0820031


Characterization of impurities in the bulk drug lisinopril by liquid chromatography/ion trap spectrometry


Author(s):  Pei-xi ZHU, Dan-hua WANG, Cui-rong SUN, Zhi-quan SHEN

Affiliation(s):  Department of Chemistry, Zhejiang University, Hangzhou 310027, China; more

Corresponding email(s):   suncuirong@zju.edu.cn, zhiquan_shen@163.com

Key Words:  Lisinopril, Impurities, High-performance liquid chromatography (HPLC), Multi-stage mass spectrometry (MSn)


Pei-xi ZHU, Dan-hua WANG, Cui-rong SUN, Zhi-quan SHEN. Characterization of impurities in the bulk drug lisinopril by liquid chromatography/ion trap spectrometry[J]. Journal of Zhejiang University Science B, 2008, 9(5): 385~390.

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author="Pei-xi ZHU, Dan-hua WANG, Cui-rong SUN, Zhi-quan SHEN",
journal="Journal of Zhejiang University Science B",
volume="9",
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year="2008",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0820031"
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%T Characterization of impurities in the bulk drug lisinopril by liquid chromatography/ion trap spectrometry
%A Pei-xi ZHU
%A Dan-hua WANG
%A Cui-rong SUN
%A Zhi-quan SHEN
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 5
%P 385~390
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820031

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T1 - Characterization of impurities in the bulk drug lisinopril by liquid chromatography/ion trap spectrometry
A1 - Pei-xi ZHU
A1 - Dan-hua WANG
A1 - Cui-rong SUN
A1 - Zhi-quan SHEN
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 5
SP - 385
EP - 390
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820031


Abstract: 
Two trace impurities in the bulk drug lisinopril were detected by means of high-performance liquid chromatography coupled with mass spectrometry (HPLC/MS) with a simple and sensitive method suitable for HPLC/MSn analysis. The fragmentation behavior of lisinopril and the impurities was investigated, and two unknown impurities were elucidated as 2-(6-amino-1-(1-carboxyethylamino)-1-oxohexan-2-ylamino)-4-phenylbutanoic acid and 6-amino-2-(1-carboxy-3-phenylpropylamino)-hexanoic acid on the basis of the multi-stage mass spectrometry and exact mass evidence. The proposed structures of the two unknown impurities were further confirmed by nuclear magnetic resonance (NMR) experiments after preparative isolation.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

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