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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.8 P.602-609

http://doi.org/10.1631/jzus.B0820120


A fusion protein containing murine vascular endothelial growth factor and tissue factor induces thrombogenesis and suppression of tumor growth in a colon carcinoma model


Author(s):  Feng-ying HUANG, Yue-nan LI, Hua WANG, Yong-hao HUANG, Ying-ying LIN, Guang-hong TAN

Affiliation(s):  Hainan Provincial Key Laboratory of Tropical Medicine, Hainan Medical College, Haikou 571101, China

Corresponding email(s):   tanhoho@163.com

Key Words:  Thrombogenesis, Vascular endothelial growth factor (VEGF), Tissue factor (TF), Recombinant fusion protein


Feng-ying HUANG, Yue-nan LI, Hua WANG, Yong-hao HUANG, Ying-ying LIN, Guang-hong TAN. A fusion protein containing murine vascular endothelial growth factor and tissue factor induces thrombogenesis and suppression of tumor growth in a colon carcinoma model[J]. Journal of Zhejiang University Science B, 2008, 9(8): 602-609.

@article{title="A fusion protein containing murine vascular endothelial growth factor and tissue factor induces thrombogenesis and suppression of tumor growth in a colon carcinoma model",
author="Feng-ying HUANG, Yue-nan LI, Hua WANG, Yong-hao HUANG, Ying-ying LIN, Guang-hong TAN",
journal="Journal of Zhejiang University Science B",
volume="9",
number="8",
pages="602-609",
year="2008",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0820120"
}

%0 Journal Article
%T A fusion protein containing murine vascular endothelial growth factor and tissue factor induces thrombogenesis and suppression of tumor growth in a colon carcinoma model
%A Feng-ying HUANG
%A Yue-nan LI
%A Hua WANG
%A Yong-hao HUANG
%A Ying-ying LIN
%A Guang-hong TAN
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 8
%P 602-609
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820120

TY - JOUR
T1 - A fusion protein containing murine vascular endothelial growth factor and tissue factor induces thrombogenesis and suppression of tumor growth in a colon carcinoma model
A1 - Feng-ying HUANG
A1 - Yue-nan LI
A1 - Hua WANG
A1 - Yong-hao HUANG
A1 - Ying-ying LIN
A1 - Guang-hong TAN
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 8
SP - 602
EP - 609
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820120


Abstract: 
Induction of tumor vasculature occlusion by targeting a thrombogen to newly formed blood vessels in tumor tissues represents an intriguing approach to the eradication of primary solid tumors. In the current study, we construct and express a fusion protein containing vascular endothelial growth factor (VEGF) and tissue factor (TF) to explore whether this fusion protein has the capability of inhibiting tumor growth in a colon carcinoma model. The murine cDNA of VEGF A and TF were amplified by reverse transcriptase polymerase chain reaction (RT-PCR), and then cloned into prokaryotic expression plasmid pQE30 with a linker. The expression product recombinant VEGF-TF (rVEGF-TF) was purified and proved to have comparable enzyme activity to a commercial TF and the capability of specific binding to tumor vessels. Significant decrease of tumor growth was found in the mice administered with rVEGF-TF on Day 6 after initiated rVEGF-TF treatment (P<0.05), and the tumor masses in 2 of 10 mice were almost disappeared on Day 14 after the first treatment. In addition, valid thrombogenesis and tumor necrosis were observed in the tumor tissues injected with rVEGF-TF. Our results demonstrate that occlusion of tumor vasculature with rVEGF-TF is potentially an effective approach for cancer therapy.

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